全文获取类型
收费全文 | 540篇 |
免费 | 31篇 |
国内免费 | 1篇 |
出版年
2021年 | 5篇 |
2018年 | 4篇 |
2017年 | 9篇 |
2016年 | 13篇 |
2015年 | 10篇 |
2014年 | 16篇 |
2013年 | 13篇 |
2012年 | 22篇 |
2011年 | 23篇 |
2010年 | 20篇 |
2009年 | 24篇 |
2008年 | 19篇 |
2007年 | 26篇 |
2006年 | 25篇 |
2005年 | 15篇 |
2004年 | 17篇 |
2003年 | 23篇 |
2002年 | 20篇 |
2001年 | 23篇 |
2000年 | 12篇 |
1999年 | 14篇 |
1998年 | 14篇 |
1997年 | 9篇 |
1996年 | 5篇 |
1994年 | 8篇 |
1993年 | 9篇 |
1992年 | 9篇 |
1991年 | 7篇 |
1990年 | 7篇 |
1989年 | 10篇 |
1988年 | 12篇 |
1987年 | 9篇 |
1986年 | 4篇 |
1985年 | 4篇 |
1984年 | 5篇 |
1983年 | 8篇 |
1982年 | 4篇 |
1981年 | 4篇 |
1978年 | 9篇 |
1977年 | 8篇 |
1975年 | 3篇 |
1974年 | 3篇 |
1973年 | 5篇 |
1971年 | 4篇 |
1969年 | 3篇 |
1961年 | 3篇 |
1958年 | 3篇 |
1951年 | 3篇 |
1948年 | 3篇 |
1935年 | 3篇 |
排序方式: 共有572条查询结果,搜索用时 796 毫秒
101.
A polymerase chain reaction (PCR) assay based on the 16S rRNA gene and an improved DNA extraction procedure were developed for the direct detection and differentiation of Campylobacter upsaliensis and C. helveticus in seeded human faeces. The PCR assay was compared with culture detection by a membrane filter (MF) technique and on selective agar (SA) containing 8 mg l−1 cefoperazone. Both MF culture and the PCR assay detected 105 colony-forming units (cfu) g−1 faeces. Selective agar culture of some strains could detect as few as 103 cfu g−1 faeces. However, some strains were susceptible to cefoperazone and either failed to grow or were detected only with reduced sensitivity in the presence of the antibiotic. Detection by MF and SA both required 48–96 h incubation in a microaerobic atmosphere and did not specifically identify the isolate. By contrast, the PCR assay could be completed within 8 h and accurately identified the two phenotypically similar species, C. upsaliensis and C. helveticus. 相似文献
102.
Current phylogenetic tree reconstruction methods assume that there is a
single underlying tree topology for all sites along the sequence. The
presence of mosaic sequences due to recombination violates this assumption
and will cause phylogenetic methods to give misleading results due to the
imposition of a single tree topology on all sites. The detection of mosaic
sequences caused by recombination is therefore an important first step in
phylogenetic analysis. A graphical method for the detection of
recombination, based on the least squares method of phylogenetic
estimation, is presented here. This method locates putative recombination
breakpoints by moving a window along the sequence. The performance of the
method is assessed by simulation and by its application to a real data set.
相似文献
103.
We used the repetitive character of transposable elements to isolate a
non-LTR retrotransposon in Drosophila subobscura. bilbo, as we have called
it, has homology to TRIM and LOA elements. Sequence analysis showed a 5'
untranslated region (UTR), an open reading frame (ORF) with no RNA-binding
domains, a downstream ORF that had structural homology to that of the I
factor, and, finally, a 3' UTR which ended in several 5-nt repeats. The
results of our phylogenetic and structural analyses shed light on the
evolution of Drosophila non-LTR retrotransposons and support the hypothesis
that an ancestor of these elements was structurally complex.
相似文献
104.
Willingness to Pay for Eco‐Certified Refurbished Products: The Effects of Environmental Attitudes and Knowledge 下载免费PDF全文
Refurbishing products, which are increasingly sold in business‐to‐consumer markets, is a key strategy to reduce waste. Nevertheless, research finds that consumers’ willingness to pay (WTP) for refurbished products is low. Strategies for a higher WTP are needed in order to grow consumer markets for refurbished products. Eco‐certification of refurbished products may be a key strategy here. Drawing on the consumer WTP literature concerning “green” products, we investigate the impact of independent eco‐certificates. Our analysis is based on a survey of 231 potential customers. The results suggest that, across various product categories, the WTP for products with refurbished components is significantly lower. Adding an eco‐certificate tends to return the WTP toward the virgin product level. We show that consumers with proenvironmental attitudes particularly exhibit green buying behavior. Our findings indicate that eco‐certification is often worthwhile because it enhances the business rationale for producing products with refurbished components. 相似文献
105.
Variation of physico-chemical parameters along a river transect through the Okavango Delta,Botswana 总被引:1,自引:0,他引:1
The Okavango Delta depends on water quantity and quality to sustain its ecosystem services. Whereas many studies have been carried out on its hydrology, few have been done on water quality in the delta. Water pH, electrical conductivity (EC), dissolved oxygen (DO), turbidity, total suspended solids (TSS) and dissolved organic carbon (DOC) were monitored at 10 sites along the Okavango–Boro–Thamalakane–Lake Ngami system almost fortnightly from June 2008 to June 2010. Water quality in the delta was generally good, despite high evapotranspiration rates which would normally produce very saline waters. Electrical conductivity and water temperature increased with distance from Mohembo to Lake Ngami, the former most likely due to evapoconcentration. In contrast, pH, DO, turbidity and TSS decreased with distance from Mohembo to Boro at the lower end of the seasonal floodplain, before increasing again to Lake Ngami. Dissolved oxygen and TSS most likely declined due to biological uptake and particle sedimentation, respectively. Strong and significant relationships were observed between TSS and turbidity and between DOC and EC, indicating that turbidity and EC could be useful proxies for routine estimations of TSS and DOC, respectively, in the delta. 相似文献
106.
The suitability of using macroinvertebrates as bioindicators of stream water quality was tested in the Mkondoa River in an agricultural area at Kilosa, using the rapid bioassessment protocol. The family biotic index (FBI) showed marked variation in water quality along the stream from values ranging from 4.1 to 5.0 in the upstream reaches, indicating good water quality, 5.3 to 5.5 in the mid-reaches and 6.0 to 6.5 in the lower reaches. The water quality index (WQI) indicated that water quality was fair (77 ± 0.98) in the upstream reach of the Mkondoa, marginal (55 ± 0.86) in the midstream reach and poor (33 ± 0.45) in the downstream reach. There were significant relationships between biological oxygen demand and dissolved oxygen and the occurrence of specific taxa, mainly Chironomus and Caenis. Significant changes in macroinvertebrate abundance were mostly related to changes in water quality. As in other parts of the world, macroinvertebrate communities proved to be good biological indicators of water quality and they should be used as bioindicators in long-term monitoring of this river. 相似文献
107.
The effect of ampicillin and tylosin on the faecal enterococci of healthy young chickens 总被引:1,自引:0,他引:1
Enterococcal isolates from young chickens were differentiated into one of three species, namely Enterococcus faecalis, Ent. faecium and Ent. gallinarum. The proportion of each species among the enterococcal population changed with time in birds not dosed with antibiotics. This pattern of change was modified in birds dosed with either tylosin or ampicillin even though ampicillin did not select for ampicillin resistance among the enterococcal population. A gradual increase in tylosin resistance was recorded with time among the enterococci of the 'undosed' control birds. This was associated with an increase in the proportion of Ent. faecium, a species commonly resistant to tylosin, among the enterococci of the birds as they grew older. 相似文献
108.
109.
110.
Cognitive Structure and Informant Accuracy 总被引:5,自引:0,他引:5
The problem of informant accuracy is examined in light of principles of memory organization from cognitive psychology. These principles turn out to be powerful, not only in explaining overall patterns of informant error, but in predicting details about the types of errors made. Predictions are made in terms both of different kinds of informants and different kinds of objects. All the predictions are strongly supported by the data. Finally, in the light of these results, two strategies are developed. The "best" informants, it seems, can be used to reveal long-range stable patterns of events, and the "worst" informants can be used to reveal the details of a particular event of special interest. 相似文献