全文获取类型
收费全文 | 1654篇 |
免费 | 172篇 |
国内免费 | 3篇 |
出版年
2022年 | 13篇 |
2021年 | 14篇 |
2020年 | 10篇 |
2019年 | 21篇 |
2018年 | 19篇 |
2017年 | 23篇 |
2016年 | 37篇 |
2015年 | 52篇 |
2014年 | 89篇 |
2013年 | 113篇 |
2012年 | 106篇 |
2011年 | 100篇 |
2010年 | 51篇 |
2009年 | 49篇 |
2008年 | 57篇 |
2007年 | 71篇 |
2006年 | 60篇 |
2005年 | 71篇 |
2004年 | 51篇 |
2003年 | 77篇 |
2002年 | 52篇 |
2001年 | 61篇 |
2000年 | 66篇 |
1999年 | 51篇 |
1998年 | 25篇 |
1997年 | 21篇 |
1996年 | 20篇 |
1995年 | 20篇 |
1994年 | 21篇 |
1993年 | 16篇 |
1992年 | 36篇 |
1991年 | 24篇 |
1990年 | 23篇 |
1989年 | 23篇 |
1988年 | 28篇 |
1987年 | 16篇 |
1986年 | 19篇 |
1985年 | 17篇 |
1984年 | 15篇 |
1982年 | 12篇 |
1981年 | 13篇 |
1980年 | 11篇 |
1979年 | 24篇 |
1978年 | 12篇 |
1977年 | 10篇 |
1976年 | 10篇 |
1975年 | 23篇 |
1974年 | 17篇 |
1973年 | 8篇 |
1972年 | 9篇 |
排序方式: 共有1829条查询结果,搜索用时 375 毫秒
251.
Johanna Lass-Hennemann Christian E. Deuter Linn K. Kuehl André Schulz Terry D. Blumenthal Hartmut Schachinger 《Proceedings. Biological sciences / The Royal Society》2010,277(1691):2175-2183
Although humans usually prefer mates that resemble themselves, mating preferences can vary with context. Stress has been shown to alter mating preferences in animals, but the effects of stress on human mating preferences are unknown. Here, we investigated whether stress alters men''s preference for self-resembling mates. Participants first underwent a cold-pressor test (stress induction) or a control procedure. Then, participants viewed either neutral pictures or pictures of erotic female nudes whose facial characteristics were computer-modified to resemble either the participant or another participant, or were not modified, while startle eyeblink responses were elicited by noise probes. Erotic pictures were rated as being pleasant, and reduced startle magnitude compared with neutral pictures. In the control group, startle magnitude was smaller during foreground presentation of photographs of self-resembling female nudes compared with other-resembling female nudes and non-manipulated female nudes, indicating a higher approach motivation to self-resembling mates. In the stress group, startle magnitude was larger during foreground presentation of self-resembling female nudes compared with other-resembling female nudes and non-manipulated female nudes, indicating a higher approach motivation to dissimilar mates. Our findings show that stress affects human mating preferences: unstressed individuals showed the expected preference for similar mates, but stressed individuals seem to prefer dissimilar mates. 相似文献
252.
253.
How many pols does it take to replicate nuclear DNA? 总被引:17,自引:0,他引:17
254.
Edward E. Nishizawa William L. Miller Robert R. Gorman Gordon L. Bundy Jan Svensson Mats Hamberg 《Prostaglandins & other lipid mediators》1975,9(1)
Prostaglandin D2 was found to be a potent inhibitor of platelet aggregation. Aggregation of human platelets by ADP, collagen and prostaglandin G2 was inhibited more strongly by PGD2 than by PGE1. Although ADP-induced aggregation of rabbit platelets was inhibited more strongly by PGE1 than by PGD2 the latter prostaglandin gave a more long-lasting inhibitory effect on platelet aggregation following intravenous or oral administration. These results coupled with the finding that PGD2 has less hypotensive effects on the cardiovascular system than PGE1 suggest the possible use of PGD2 as an antithrombotic agent. 相似文献
255.
256.
257.
Srikanth Mairpady Shambat Axana Haggar Francois Vandenesch Gerard Lina Willem J. B. van Wamel Gayathri Arakere Mattias Svensson Anna Norrby-Teglund 《PloS one》2014,9(8)
Epidemiological studies of Staphylococcus aureus have shown a relation between certain clones and the presence of specific virulence genes, but how this translates into virulence-associated functional responses is not fully elucidated. Here we addressed this issue by analyses of community-acquired S. aureus strains characterized with respect to antibiotic resistance, ST types, agr types, and virulence gene profiles. Supernatants containing exotoxins were prepared from overnight bacterial cultures, and tested in proliferation assays using human peripheral blood mononuclear cells (PBMC). The strains displayed stable phenotypic response profiles, defined by either a proliferative or cytotoxic response. Although, virtually all strains elicited superantigen-mediated proliferative responses, the strains with a cytotoxic profile induced proliferation only in cultures with the most diluted supernatants. This indicated that the superantigen-response was masked by a cytotoxic effect which was also confirmed by flow cytometry analysis. The cytotoxic supernatants contained significantly higher levels of α-toxin than did the proliferative supernatants. Addition of α-toxin to supernatants characterized as proliferative switched the response into cytotoxic profiles. In contrast, no effect of Panton Valentine Leukocidin, δ-toxin or phenol soluble modulin α-3 was noted in the proliferative assay. Furthermore, a significant association between agr type and phenotypic profile was found, where agrII and agrIII strains had predominantly a proliferative profile whereas agrI and IV strains had a predominantly cytotoxic profile. The differential response profiles associated with specific S. aureus strains with varying toxin production could possibly have an impact on disease manifestations, and as such may reflect specific pathotypes. 相似文献
258.
Linn S. Lehnert Stephanie Kramer-Schadt Sophia Sch?nborn Oliver Lindecke Ivo Niermann Christian C. Voigt 《PloS one》2014,9(8)
Over recent years, it became widely accepted that alternative, renewable energy may come at some risk for wildlife, for example, when wind turbines cause large numbers of bat fatalities. To better assess likely populations effects of wind turbine related wildlife fatalities, we studied the geographical origin of the most common bat species found dead below German wind turbines, the noctule bat (Nyctalus noctula). We measured stable isotope ratios of non-exchangeable hydrogen in fur keratin to separate migrants from local individuals, used a linear mixed-effects model to identify temporal, spatial and biological factors explaining the variance in measured stable isotope ratios and determined the geographical breeding provenance of killed migrants using isoscape origin models. We found that 72% of noctule bat casualties (n = 136) were of local origin, while 28% were long-distance migrants. These findings highlight that bat fatalities at German wind turbines may affect both local and distant populations. Our results indicated a sex and age-specific vulnerability of bats towards lethal accidents at turbines, i.e. a relatively high proportion of killed females were recorded among migratory individuals, whereas more juveniles than adults were recorded among killed bats of local origin. Migratory noctule bats were found to originate from distant populations in the Northeastern parts of Europe. The large catchment areas of German wind turbines and high vulnerability of female and juvenile noctule bats call for immediate action to reduce the negative cross-boundary effects of bat fatalities at wind turbines on local and distant populations. Further, our study highlights the importance of implementing effective mitigation measures and developing species and scale-specific conservation approaches on both national and international levels to protect source populations of bats. The efficacy of local compensatory measures appears doubtful, at least for migrant noctule bats, considering the large geographical catchment areas of German wind turbines for this species. 相似文献
259.
Exposure of HeLa DNA polymerase alpha to protein kinase C affects its catalytic properties 总被引:3,自引:0,他引:3
S W Krauss D Mochly-Rosen D E Koshland S Linn 《The Journal of biological chemistry》1987,262(8):3432-3435
Protein kinase C (Ca2+/phospholipid-dependent protein kinase) purified from rat brain or endogenous to cell-free extracts from HeLa cells stimulates, by a factor of 2-3, HeLa DNA polymerase alpha but not beta or gamma. Monoclonal antibody to the kinase prevents the stimulation, and monoclonal antibody to human DNA polymerase alpha neutralizes the enhanced activity. Reduced DNA polymerase alpha activity is obtained from noncycling HeLa cells and this activity has lower fidelity when copying synthetic primer-templates than that obtained from log phase cultures. After exposure to the kinase, the fidelities and activities of the polymerase from both sources increase by 2- to 3-fold. This improved accuracy is not accompanied by the appearance of triphosphatase or DNase activities. Exposure to the protein kinase reduces the Km for activated DNA and for poly(dA-dT) but not for dNTPs. Moreover, the Vmax for activated DNA but not for poly(dA-dT) is increased approximately 2- to 3-fold. These alterations suggest a role for protein phosphorylation in modulating DNA polymerase alpha. 相似文献
260.