FOUR NEW SPECIES OF COENOSIA MEIGEN (DIPTERA: MUSCIDAE) FROM YUNNAN, CHINA

Abstract  This paper describes four new species of Coenosia Meigen, 1826, namely C. angustifolia sp.nov., C. obscuriabdominis sp. nov., C. sparagmocerca sp. nov. and C. sponsa sp. nov. Type specimens are deposited in Institute of Entomology, Shenyang Normal University, Shenyang, China.     

湿度和温度对酰化紫膜LB膜M衰减的影响

用闪光动力学光谱仪测量了酰化紫膜ＬＢ膜中Ｍ衰减速率的变化。酰化紫膜ＬＢ膜的衰减无论是悬浮液状态,还是ＬＢ膜中,均比未修饰的要慢。在温度为２０℃时,酰化紫膜ＬＢ随着相对湿度的增加,Ｍ衰减加快。在相对湿度较低时（ＲＨ３４—７５％）,变化较平缓,即Ｍ的衰减加快不明显;在相对湿度较高时（ＲＨ８４—９５％）,Ｍ衰减明显加快。温度的变化则随相对湿度不同而不同。相对湿度较低时,随着温度的升高,Ｍ衰减加快;相对湿度较高时,Ｍ衰减反而减慢。酰化紫膜悬浮液的Ｍ衰减随着温度的升高而明显加快．这说明酰化紫膜ＬＢ膜中ＢＲ水合程度可能是直接影响Ｍ衰减的因素之一。     

四种水蛭抗凝血酶作用的研究

本文研究了水蛭的抗凝血酶作用,并比较了4种提取方法对抗凝作用的影响。冷浸和温浸,抗凝活性强度为菲牛蛭>日本医蛭》两种宽体金线蛭。煎煮后,前两种的活性锐碱,而两种宽体金线蛭的活性几乎不变。     

铽离子与RNA的荧光反应及RNA测定的研究

核糖核酸（ＲＮＡ）与稀土铽离子在ｐＨ５．０～６．５条件下能形成具有较强荧光的络合物,其激发峰在２８８ｎｍ处,发射峰为４９４ｎｍ及５４５ｎｍ处．ＲＮＡ在０．１～１０ｍｇ／Ｌ范围内与其荧光强度呈线性关系,其检出限为６．０×１０￣（－８）ｍｏｌ／Ｌ．腺苷酸,尿苷酸,胞苷酸对ＲＮＡ的干扰比较小,因此可在其存在下选择性地测定ＲＮＡ．     

Identification of the Sites for Suppressor Mutations on the Hemagglutinin Molecule to Temperature-Sensitive Phenotype of the Influenza Virus

A temperature-sensitive (ts) mutant of the influenza virus A/WSN/ 33 strain, ts-134, possessed a defect in intracellular transport at the nonpermissive temperature and marked thermolability of hemagglutinin (HA) activity at 51 C. These were caused by a change at amino acid residue 157 from tyrosine to histidine in the HA protein. We isolated 37 spontaneous revertant clones from ts-134 at the nonpermissive temperature and determined their HA sequences. The deduced amino acid sequences demonstrated that one was a true revertant and the others were revertants with suppressor mutations, each of which had an additional amino acid change besides those of ts-134. The changed amino acids were located at 14 positions on the HA molecule, and eight of them were found in multiple revertants. These were located in five to six distinct regions on the three-dimensional structure of the HA molecule. However, the heat stability of HAs in the revertants was recovered differently depending on the sites of the changed amino acids. The kinetics of transport of the HA protein in the revertants were slightly delayed compared to the wild-type both at permissive and nonpermissive temperatures.     

Characterization and immunocytochemical demonstration of glucocorticoid receptor using antisera specific to transformed receptor

Cytosolic and nuclear forms of the glucocorticoid receptor were characterized using immunochemical techniques. Antibodies were raised in rabbits to an M_r 58,000 fragment of the transformed (DNA-binding) glucocorticoid receptor purified from rat liver cytosol by DNA-cellulose chromatography and polyacrylamide gel electrophoresis. Antibodies reacted with the transformed receptor form in a radioimmunoassay for glucocorticoid receptor. Western blot analysis of antibody reactivity revealed a single M_r 185,000 receptor form in rat liver cytosol but a smaller M_r 85,000 form in nucleosol, indicating the M_r 85,000 form is the transformed receptor. Furthermore, western blot analysis indicates that the M_r 185,000 receptor undergoes proteolysis during receptor purification and in vitro transformation processes by generating immunochemically similar proteins of smaller molecular weights. An identical M_r 185,000 glucocorticoid receptor was detected in cytosols of four rat tissues; liver, brain, adrenal medulla, and thymus. The glucocorticoid receptor was localized to the cytoplasm and nucleus of rat adrenal medulla cells by immunohistochemistry, demonstrating the existence in vivo of the transformed receptor and translocation of the receptor from cytoplasm to nucleus.     

眼底图象定量分析

本文介绍了基于数学形态学的图象分析系统在生物医学,特别是眼底图方面日益广泛的应用.提出了运用图象分析系统取得眼底照片上一些参数的方法.主要参数包括:动静脉管径及其比例,无灌注区的面积等.这些参数不仅用于眼科方面的诊断和治疗,对于分析和诊断全身性疾病,如高血压、动脉硬化、糖尿病、静脉阻塞等都具有重要价值