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Chen Q Xie LX Qing ZR Li LJ Luo ZY Lin M Zhang SM Chen WZ Lin BZ Lin QL Li H Chen WP Zheng PY Mao LZ Chen CY Yang C Zhan YZ Liu XZ Zheng JK Yang LY 《PloS one》2012,7(2):e32149
Background
Human papilloma virus (HPV) infection was the main cause of cervical cancer. There were only a few reports and detailed data about epidemiological research of HPV infection in rural population of China.Materials and Methods
The cervical cells of rural Chaozhou women were collected, and multiplex real time PCR was firstly performed to detect high-risk HPV (HR-HPV) infection, which could detect 13 types of HR-HPV (types 16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, and 68). Then, HPV-positive samples were typed by HPV GenoArray test.Results
HR-HPV DNA was detected by multiplex real time-PCR in 3830 of 48559 cases (7.89%). There was a peak incidence in age of 55–60 years group, and a lower incidence in who lived in plain group compared with suburban, mountain and seashore group. 3380 cases of HPV positive sample were genotyped, 11.01% (372/3380) cases could not be classified, among the typed 3008 cases, 101 cases were identified without HR-HPV type infection, 2907 cases were infected with one HR-HPV type at least, the 6 most common HR-HPV types in descending order of infection, were type 52 (33.4%, 16 (20.95%), 58 (15.93%), 33 (9.94%), 68 (9.22%) and 18 (8.36%). The combined prevalence of HPV types 16 and 18 accounted for 28.52% of total infection. However, type 52 plus 58 presented 48.23% of total infection. 2209/2907 cases were infected with a single HPV type and 698/2907 cases were infected with multiple types, and multiple infection constituent ratio increased with age, with a peak incidence in age 55–60 years group.Conclusions
Our findings showed low prevalence of HPV vaccine types (16 and 18) and relatively high prevalence of HPV-52 and -58, support the hypothesis that the second-generation HPV vaccines including HPV-52 and -58 may offer higher protection for women in rural Guangdong Province. 相似文献42.
适应不同光环境小蜡叶片气孔导度、气孔开度和气孔密度的时空变化 总被引:7,自引:0,他引:7
对生长在强光环境和弱光环境小蜡叶片的气孔参数测定发现 :气孔导度和气孔开度在 4个取样部位存在异质性分布。气孔导度和气孔开度经回归分析呈线性、指数或多项式分布。统计分析表明 :强光下的叶片气孔导度和气孔开度的相关性明显高于弱光环境叶片的数值。无论强光环境还是弱光环境下的叶片 ,在取样部位编号为1和 3,其气孔导度和气孔开度均存在显著的线性关系。弱光环境下叶片的气孔密度要远低于强光环境下的叶片。强光环境下叶片对变化环境的敏感性要大于弱光环境下的叶片 ,这可能与强光环境叶片具有较高的气孔密度有关。 相似文献
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旅游对北京东灵山亚高山草甸物种多样性影响的初步研究 总被引:33,自引:0,他引:33
在1965、1983、1994和2000年4个时期调查数据的基础上,对北京东灵山区亚高山草甸物种多样性变化和生活型谱进行了对比研究,结果显示,近年来东灵山亚高山草甸的物种多样性呈下降趋势,尤其是1983~1994年的下降速度明显加快,利用重要值作为权重参数对不同时期的东灵山亚高山草甸生活型谱进行了比较,所得到的结果反映了20世纪80年代以来,伴随旅游发展而迅速增加的马匹散牧已经对亚高山草甸的群落外貌造成了严重的不良影响,群落高度降低,物种组成改变及植被盖度下降等,说明过度放牧是东灵山亚高山草甸退化的重要影响因素,根据东灵山风景区亚高山草甸目前存在的问题,我们建议当地有关部门科学地确定东灵山风景区旅游容量,首先要严格限制风景区马匹的散牧,关闭风景区内的跑马场和牧马帐蓬,并通过加强宣传、管理和景区的基本建设,充分发挥生态系统的服务功能,实现在保护生态环境的前提下实现资源的持续利用。 相似文献
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在夏季的晴天阳生叶和阴生叶方位角和方向角的日变化均很小,但是叶片的中脉角存在显著的日变化差异,这种叶片垂直方向上的显著运动与躲避中午的强光胁迫有关.而在阴天,阳生叶和阴生叶方位角、方向角和中脉角的日变化均很小.这说明变化的光环境是引起刺槐复叶运动的主要原因,而节律性运动不是刺槐复叶运动的诱导因素.机械固定叶片的控制实验显示:机械固定叶片的光合速率、气孔导度和蒸腾速率均显著低于对照叶片,而机械固定叶片温度明显高于对照叶片,这可能是由于机械固定叶片和对照叶片两者在光辐射接受量上的差异造成的.光诱导下的刺槐复叶运动是植株维持最佳生理状态的形态调节表现. 相似文献
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Litter bag method was used in this study on the twig decomposition of an oak ( Quercus liaotungensis Koidz. ) which is dominant in the warm temperate deciduous forests. A consecutive five-year investigation was carded out to measure the changes of organic components in the twig litter. The decomposition of oak twigs based on rates of the mass loss during the first five years was simulated using the Olson exponential equation. The simulated data fit well with the observed values. Oak twigs were predicted to reach 95% decomposition within 21 years. During the first five years, the concentration of protein in the remaining litter increased from 3.5 % to 5.5 %, while the concentration of semicellulose decreased from 16.0% to 8.0%. However, there was no obvious change in the concentrations of lignin and cellulose. The loss of lignin, crude-cellulose, cellulose and semicellulose could be well simulated using the Olson exponential equation. However, this was not so effective for protein. 相似文献
47.
通过对蒙古栎红松林3个年龄阶段物种和结构动态的研究,结果表明,该类型森林的更新状况良好,早期阶段红松(Pinus koraiensis)和阔叶树的更新数量相同,后期更新树种以红松为主;在森林发育早期阶段蒙古栎(Quercus mongolica)等阳性树种占优势,中期为红松和阔叶树占优势的混交林,后期形成红松占优势的林分;随着森林的发育,灌木和草本层动态呈现复杂的变化。通过对该类型森林直径分布变化的研究得出,在林分发育的早期,阔叶树中大径级木较多,针叶树中小径级木多,在林分发育的后期呈相反的规律。用理论概率模型拟合直径分布表明,韦布尔概率分布模型是描述蒙古栎红松林直径分布的最适模型。 相似文献
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Based on plot investigation, stem analysis and radial growth pattern, the authors studied the history of suppression, release and regeneration strategies of Fagus engleriana Seem. and Quercus aliena var. acuteserrata Maxim. forests, which were widely distributed in Shennongjia of Hubei Province. It was found that (85.9 ± 6.9) % of the Fagus engleriana samples showed periods of suppression during their canopy recruitment. The average number of suppression periods was 2.1 ± 0.8, the average total length of suppression time was (47 ± 24.1) a, the length of longest suppression time was 73 a, the average number of periods of release was 1.6±0.7, and the average total length of release time was 23 a. (60.83±17.3)% of the Quercus aliena var. acuteserrata samples showed periods of suppression but without release. Combined with height and radial growth characteristics, these suggest that Fagus engleriana was shade-tolerant species, and its regeneration strategies was release from seedling sprouts in canopy gaps, while Quercus aliena var. acuteserrata was shade-intolerant species, and its regeneration strategies was seedling establishment and growth in large canopy gaps by producing large amount of seeds. 相似文献
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CD147对白血病细胞U937生长和肿瘤形成的影响 总被引:1,自引:0,他引:1
目的:研究CD147对白血病细胞U937生长和肿瘤形成的影响。方法:分别采用脂多糖(LPS)或CD147单克隆抗体处理U937细胞;用RT-PCR和流式细胞术分别在mRNA和蛋白水平检测各组中CD147的表达情况;用流式细胞术检测在LtX3和CD147单克隆抗体作用下U937细胞周期的变化;用MTT法对各组细胞的生长状况进行分析;将细胞经皮下接种于裸鼠体内,对各组间肿瘤生长速度、肿瘤体积及裸鼠存活时间进行统计分析。结果:LPS在体外能够诱导自血病细胞U937表面CD147的表达,同时细胞增殖旺盛,但细胞凋亡数增加;使用CD147抗体阻断CD147后,能够将细胞周期阻断在G0/G1期,细胞活力下降,并诱导细胞凋亡;CD147抗体体外预处理能够抑制U937细胞在裸鼠体内的生长,使小鼠存活时间延长。结论:LPS可诱导U937细胞表面CD147分子表达增加,从而促进U937细胞的生长和肿瘤形成。 相似文献