全文获取类型
收费全文 | 4463篇 |
免费 | 509篇 |
国内免费 | 1篇 |
出版年
2021年 | 74篇 |
2020年 | 38篇 |
2019年 | 57篇 |
2018年 | 52篇 |
2017年 | 46篇 |
2016年 | 88篇 |
2015年 | 153篇 |
2014年 | 154篇 |
2013年 | 203篇 |
2012年 | 244篇 |
2011年 | 250篇 |
2010年 | 140篇 |
2009年 | 143篇 |
2008年 | 186篇 |
2007年 | 187篇 |
2006年 | 169篇 |
2005年 | 157篇 |
2004年 | 127篇 |
2003年 | 146篇 |
2002年 | 113篇 |
2001年 | 104篇 |
2000年 | 112篇 |
1999年 | 99篇 |
1998年 | 51篇 |
1997年 | 48篇 |
1996年 | 58篇 |
1995年 | 38篇 |
1994年 | 46篇 |
1993年 | 59篇 |
1992年 | 96篇 |
1991年 | 94篇 |
1990年 | 98篇 |
1989年 | 77篇 |
1988年 | 53篇 |
1987年 | 65篇 |
1986年 | 61篇 |
1985年 | 85篇 |
1984年 | 57篇 |
1983年 | 56篇 |
1982年 | 50篇 |
1981年 | 42篇 |
1980年 | 55篇 |
1979年 | 55篇 |
1978年 | 44篇 |
1977年 | 39篇 |
1975年 | 51篇 |
1974年 | 37篇 |
1973年 | 42篇 |
1972年 | 33篇 |
1971年 | 32篇 |
排序方式: 共有4973条查询结果,搜索用时 437 毫秒
131.
132.
Logan J. Pallin Nick M. Kellar Debbie Steel Natalia Botero-Acosta C. Scott Baker Jack A. Conroy Daniel P. Costa Chris M. Johnson David W. Johnston Ross C. Nichols Doug P. Nowacek Andrew J. Read Oksana Savenko Oscar M. Schofield Sharon E. Stammerjohn Deborah K. Steinberg Ari S. Friedlaender 《Global Change Biology》2023,29(8):2108-2121
The krill surplus hypothesis of unlimited prey resources available for Antarctic predators due to commercial whaling in the 20th century has remained largely untested since the 1970s. Rapid warming of the Western Antarctic Peninsula (WAP) over the past 50 years has resulted in decreased seasonal ice cover and a reduction of krill. The latter is being exacerbated by a commercial krill fishery in the region. Despite this, humpback whale populations have increased but may be at a threshold for growth based on these human-induced changes. Understanding how climate-mediated variation in prey availability influences humpback whale population dynamics is critical for focused management and conservation actions. Using an 8-year dataset (2013–2020), we show that inter-annual humpback whale pregnancy rates, as determined from skin-blubber biopsy samples (n = 616), are positively correlated with krill availability and fluctuations in ice cover in the previous year. Pregnancy rates showed significant inter-annual variability, between 29% and 86%. Our results indicate that krill availability is in fact limiting and affecting reproductive rates, in contrast to the krill surplus hypothesis. This suggests that this population of humpback whales may be at a threshold for population growth due to prey limitations. As a result, continued warming and increased fishing along the WAP, which continue to reduce krill stocks, will likely impact this humpback whale population and other krill predators in the region. Humpback whales are sentinel species of ecosystem health, and changes in pregnancy rates can provide quantifiable signals of the impact of environmental change at the population level. Our findings must be considered paramount in developing new and more restrictive conservation and management plans for the Antarctic marine ecosystem and minimizing the negative impacts of human activities in the region. 相似文献
133.
Lisong Hu Zhongping Xu Rui Fan Guanying Wang Fuqiu Wang Xiaowei Qin Lin Yan Xunzhi Ji Minghui Meng Soonliang Sim Wei Chen Chaoyun Hao Qinghuang Wang Huaguo Zhu Shu Zhu Pan Xu Hui Zhao Keith Lindsey Henry Daniell Jonathan F. Wendel Shuangxia Jin 《Plant biotechnology journal》2023,21(1):78-96
Zanthoxylum armatum and Zanthoxylum bungeanum, known as ‘Chinese pepper’, are distinguished by their extraordinary complex genomes, phenotypic innovation of adaptive evolution and species-special metabolites. Here, we report reference-grade genomes of Z. armatum and Z. bungeanum. Using high coverage sequence data and comprehensive assembly strategies, we derived 66 pseudochromosomes comprising 33 homologous phased groups of two subgenomes, including autotetraploid Z. armatum. The genomic rearrangements and two whole-genome duplications created large (~4.5 Gb) complex genomes with a high ratio of repetitive sequences (>82%) and high chromosome number (2n = 4x = 132). Further analysis of the high-quality genomes shed lights on the genomic basis of involutional reproduction, allomones biosynthesis and adaptive evolution in Chinese pepper, revealing a high consistent relationship between genomic evolution, environmental factors and phenotypic innovation. Our study provides genomic resources and new insights for investigating diversification and phenotypic innovation in Chinese pepper, with broader implications for the protection of plants under severe environmental changes. 相似文献
134.
Kayleigh M. Hansford Sara L. Gandy Emma L. Gillingham Liz McGinley Benjamin Cull Colin Johnston Matthew Catton Jolyon M. Medlock 《Medical and veterinary entomology》2023,37(1):152-163
Tick-borne disease risk is intrinsically linked to the distribution of tick vector species. To assess risk and anticipate disease emergence, an understanding of tick distribution, host associations, and seasonality is needed. This can be achieved, to some extent, using passive surveillance supported by engagement with the public, animal health, and public health experts. The Tick Surveillance Scheme (TSS) collects data and maps tick distribution across the United Kingdom (UK). Between 2017 and 2020, 3720 tick records were received and 39 tick species were detected. Most records were acquired in the UK, with a subset associated with recent overseas travel. The dominant UK acquired species was Ixodes ricinus (Ixodida: Ixodidae, Linnaeus), the main vector of Lyme borreliosis. Records peaked during May and June, highlighting a key risk period for tick bites. Other key UK species were detected, including Dermacentor reticulatus (Ixodida: Ixodidae, Fabricius) and Haemaphysalis punctata (Ixodida: Ixodidae, Canestrini & Fanzago) as well as several rarer species that may present novel tick-borne disease risk to humans and other animals. Updated tick distribution maps highlight areas in the UK where tick exposure has occurred. There is evidence of increasing human tick exposure over time, including during the COVID-19 pandemic, but seasonal patterns remain unchanged. 相似文献
135.
136.
Martin J. Michaelson H. James Price John R. Ellison J. Spencer Johnston 《American journal of botany》1991,78(2):183-188
An improved procedure is reported for determining DNA amounts of plant nuclei. Nuclei stained with propidium iodide, isolated from chopped plant leaves, were passed through an Ortho Cytofluorograph with a Lexel model 95 argon laser (514 nm) and the fluorescence measured, integrated, and recorded using an Ortho 2140 Data Acquisition computer. All nuclear samples were mixed with nuclei of Sultan barley (2C DNA content = 11.12 pg [picogram]) as an internal standard. DNA contents of ten plant species, ranging from 2C = 1.7 pg to 36.1 pg measured by flow cytometry, correlated strongly (r = 0.99, slope = + 1.00) with DNA contents determined from Feulgen-stained nuclei of the same species using microspectrophotometry. The flow cytometric procedures were sufficiently sensitive to detect differences in DNA content between inbred lines of corn and their F1 hybrids. Our results obtained with improved procedures, specifically using propidium iodide as a fluorochrome and plant nuclei instead of chicken erythrocytes as an internal standard, demonstrate that laser flow cytometry can be a precise, rapid, and reliable method for determining nuclear DNA content of plants. 相似文献
137.
138.
The use of bulk segregant analysis to identify a RAPD marker linked to leaf rust resistance in barley 总被引:4,自引:0,他引:4
D. M. E. Poulsen R. J. Henry R. P. Johnston J. A. G. Irwin R. G. Rees 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1995,91(2):270-273
An F2 population from a cross between barley accession Q21861 and the Australian barley variety Galleon was used to develop RAPD markers for resistance to barley leaf rust (Puccinia hordei). Resistant and susceptible DNA bulks were constructed following the classification of F2 plants by leaf rust infection type. Bulked segregant analysis was then used to identify a 2.7-kb marker, designated OU022700 and located approximately 12cM from RphQ, a leaf rust resistance gene in Q21861. The marker was generated by PCR with the oligonucleotide primer OPU-02 (Operon). Infection types of F3 progeny were used to confirm assignment of F2 genotypes. OU022700 was shown, retrospectively, to be useful in the identification of individual F2 plants that had been originally misclassified as having susceptible infection types. Both the RAPD marker and RphQ will be potentially useful in the development of new barley cultivars. 相似文献
139.
NMR solution structure of a dsRNA binding domain from Drosophila staufen protein reveals homology to the N-terminal domain of ribosomal protein S5. 总被引:7,自引:3,他引:4
下载免费PDF全文
![点击此处可从《The EMBO journal》网站下载免费的PDF全文](/ch/ext_images/free.gif)
The double-stranded RNA binding domain (dsRBD) is an approximately 65 amino acid motif that is found in a variety of proteins that interact with double-stranded (ds) RNA, such as Escherichia coli RNase III and the dsRNA-dependent kinase, PKR. Drosophila staufen protein contains five copies of this motif, and the third of these binds dsRNA in vitro. Using multinuclear/multidimensional NMR methods, we have determined that staufen dsRBD3 forms a compact protein domain with an alpha-beta-beta-beta-alpha structure in which the two alpha-helices lie on one face of a three-stranded anti-parallel beta-sheet. This structure is very similar to that of the N-terminal domain of a prokaryotic ribosomal protein S5. Furthermore, the consensus derived from all known S5p family sequences shares several conserved residues with the dsRBD consensus sequence, indicating that the two domains share a common evolutionary origin. Using in vitro mutagenesis, we have identified several surface residues which are important for the RNA binding of the dsRBD, and these all lie on the same side of the domain. Two residues that are essential for RNA binding, F32 and K50, are also conserved in the S5 protein family, suggesting that the two domains interact with RNA in a similar way. 相似文献
140.
Identification and characterization of Uss1p (Sdb23p): a novel U6 snRNA-associated protein with significant similarity to core proteins of small nuclear ribonucleoproteins. 总被引:16,自引:4,他引:12
下载免费PDF全文
![点击此处可从《The EMBO journal》网站下载免费的PDF全文](/ch/ext_images/free.gif)
The SDB23 gene of Saccharomyces cerevisiae was isolated in a search for high copy-number suppressors of mutations in a cell cycle gene, DBF2, SDB23 encodes a 21,276 Da protein with significant sequence similarity to characterized mammalian snRNP core proteins. Examination of multiple sequence alignments of snRNP core proteins with Sdb23p indicates that all of these proteins share a number of highly conserved residues, and identifies a novel motif for snRNP core proteins. Sdb23p is essential for cell viability and is required for nuclear pre-mRNA splicing both in vivo and in vitro. Extracts prepared from Sdb23p-depleted cells are unable to support splicing and have vastly reduced levels of U6 snRNA. The stability of U1, U2, U4 and U5 spliceosomal snRNAs is not affected by the loss of Sdb23p. Antibodies raised against Sdb23p strongly coimmunoprecipitate free U6 snRNA and U4/U6 base-paired snRNAs. These results establish that SDB23 encodes a novel U6 snRNA-associated protein that is essential for the stability of U6 snRNA. We therefore propose the more logical name USS1 (U-Six SnRNP) for this gene. 相似文献