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81.
Formation of the CO compound has been studied in intact mitochondria, submitochondrial particles and isolated cytochrome oxidase. The reaction requires the prior reduction of both cytochrome a3 and one other single-electron acceptor. It is inferred that the second acceptor is the “invisible” copper which is undetectable by both optical and spin resonance spectroscopy. The overall process can be viewed as two single electron steps plus a ligand binding reaction. At high concentrations of CO, when titrations are performed at oxidation-reduction potentials significantly above the midpoints of either cytochrome a3 or “invisible” copper, appearance of the CO compound follows a strict n = 2 (2-electron) relationship. Its midpoint potential is also dependent on the prevailing concentration of CO and is increased by approx. 30 mV for each tenfold increase in the level of CO. At redox potentials approaching the midpoints of cytochrome a3 or “invisible” copper, significant deviations from n = 2 behavior are apparent which are readily detectable experimentally using low CO concentrations.A mathematical analysis of this model is presented and the oxidation-reduction properties of the CO compound are utilized to determine the midpoint potential of the “invisible” copper. This value is estimated to be 340 ± 10 mV at pH 7.8, independent of pH and the prevailing ratio.By analogy with the observations on CO binding, the primary intermediate in the oxidase reaction with oxygen is concluded to be a bridged a32+-O2-Cu1+ complex. The initial reduction of molecular oxygen can then proceed via a thermodynamically favorable two-electron step to form a bridged peroxide intermediate. Subsequent reduction to water may later occur by way of two single-electron steps or one two-electron step. 相似文献
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84.
Hydroxyurea reversal of inhibition and use as a cell-synchronizing agent 总被引:15,自引:0,他引:15
85.
Lindsay D. Plank W.C. Hymeer M.Elaine Kunze Gary M. Marks J.Wayne Lanham Paul Todd 《Journal of biochemical and biophysical methods》1983,8(4):275-289
Growth hormone secreting cells of the rat anterior pituitary are heavily laden with granules of growth hormone and can be partially purified on the basis of their resulting high density. Two methods of preparative cell electrophoresis were investigated as methods of enhancing the purification of growth hormone producing cells: density gradient electrophoresis and continuous flows electrophoresis. Both methods provided a two- to four-fold enrichment in growth hormone production per cell relative to that achieved by previous methods. Measurements of electrophoretic mobilities by two analytical methods, microscopic electrophoresis and laser-tracking electrophoresis, revealed very little distinction between unpurified anterior pituitary cell suspensions and somatotroph-enriched cell suspensions. Predictions calculated on the basis of analytical electrophoretic data are consistent with the hypothesis that sedimentation plays a significant role in both types of preparative electrophoresis and the electrophoretic mobility of the growth hormone secreting subpopulation of cells remains unknown. 相似文献
86.
Lindsay A. G. Davidson 《BMJ (Clinical research ed.)》1953,2(4849):1300-1303
87.
James J. Rothwell John B. Lindsay Martin G. Evans Timothy E.H. Allott 《Ecological Engineering》2010,36(5):623-630
Upland peat soils in close proximity to urban and industrial areas can be contaminated with high concentrations of atmospherically deposited lead. The peat soils of the Peak District (UK) are characterised by extensive eroding gullies. Fine-resolution digital topographic data were used to map the extent and depth of these gullies. Peat samples from eroding gully walls and suspended sediments were collected and analysed for lead content. Variability in lead concentrations of gully wall material and suspended sediments can be explained by differences in mean upslope gully depth. The lead content of suspended sediment exported from catchments characterised by shallow peat gullies is higher than that exported from catchments with deep peat gullies. The empirical relationship between sediment-associated lead concentration and mean upslope gully depth was combined with the gully depth mapping to produce a predictive spatial model of suspended sediment lead concentrations across the Peak District. This model may be particularly useful for catchment managers who are currently involved in the restoration of eroding peat soils in the Peak District uplands. 相似文献
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Sigit D. Sasmito Mriadec Sillanp Matthew A. Hayes Samsul Bachri Meli F. Saragi‐Sasmito Frida Sidik Bayu B. Hanggara Wolfram Y. Mofu Victor I. Rumbiak Hendri Sartji Taberima Suhaemi Julius D. Nugroho Thomas F. Pattiasina Nuryani Widagti Barakalla Joeni S. Rahajoe Heru Hartantri Victor Nikijuluw Rina N. Jowey Charlie D. Heatubun Philine zu Ermgassen Thomas A. Worthington Jennifer Howard Catherine E. Lovelock Daniel A. Friess Lindsay B. Hutley Daniel Murdiyarso 《Global Change Biology》2020,26(5):3028-3039
Globally, carbon‐rich mangrove forests are deforested and degraded due to land‐use and land‐cover change (LULCC). The impact of mangrove deforestation on carbon emissions has been reported on a global scale; however, uncertainty remains at subnational scales due to geographical variability and field data limitations. We present an assessment of blue carbon storage at five mangrove sites across West Papua Province, Indonesia, a region that supports 10% of the world's mangrove area. The sites are representative of contrasting hydrogeomorphic settings and also capture change over a 25‐years LULCC chronosequence. Field‐based assessments were conducted across 255 plots covering undisturbed and LULCC‐affected mangroves (0‐, 5‐, 10‐, 15‐ and 25‐year‐old post‐harvest or regenerating forests as well as 15‐year‐old aquaculture ponds). Undisturbed mangroves stored total ecosystem carbon stocks of 182–2,730 (mean ± SD: 1,087 ± 584) Mg C/ha, with the large variation driven by hydrogeomorphic settings. The highest carbon stocks were found in estuarine interior (EI) mangroves, followed by open coast interior, open coast fringe and EI forests. Forest harvesting did not significantly affect soil carbon stocks, despite an elevated dead wood density relative to undisturbed forests, but it did remove nearly all live biomass. Aquaculture conversion removed 60% of soil carbon stock and 85% of live biomass carbon stock, relative to reference sites. By contrast, mangroves left to regenerate for more than 25 years reached the same level of biomass carbon compared to undisturbed forests, with annual biomass accumulation rates of 3.6 ± 1.1 Mg C ha?1 year?1. This study shows that hydrogeomorphic setting controls natural dynamics of mangrove blue carbon stocks, while long‐term land‐use changes affect carbon loss and gain to a substantial degree. Therefore, current land‐based climate policies must incorporate landscape and land‐use characteristics, and their related carbon management consequences, for more effective emissions reduction targets and restoration outcomes. 相似文献
90.
Lindsay P. Chiquoine Scott R. Abella Joshua L. Greenwood Adria DeCorte 《Restoration Ecology》2020,28(Z2):S32-S44
Carbon addition has been proposed as an alternative to herbicide and manual removal methods to treat non‐native plants and reduce non‐target effects of treatments (e.g. impacts on native plants; surface disturbance). On Mojave Desert pavement and biocrust substrates after experimental soil disturbance and carbon addition (1,263 g C/m2 as sucrose), we observed declines in lichens and moss cover in sucrose‐treated plots. To further explore this unforeseen potential side effect of using carbon addition as a non‐native plant treatment, we conducted biocrust surveys 5 and 7 years after treatments, sampled surface soils to observe if treatments additionally affected soil filamentous cyanobacteria, and conducted laboratory trials testing the effects of different levels of sucrose on cyanobacteria and desert mosses. Sucrose addition to biocrust plots reduced lichen and moss cover by 33–78% and species richness by 40–80%. Sucrose reduced biocrust cover in biocrust plots to levels similarly detected in pavement plots (<1%). While cyanobacteria in the field did not appear to be affected by sucrose, laboratory tests showed negative effects of sucrose on both cyanobacteria and mosses. Cyanobacteria declined by 41% 1 month after exposure to 5.4 g C/m2 equivalent solutions. We detected injury to photosynthesis in mosses after 96 hour exposure to 79–316 g C/m2 equivalent solutions. Caution is warranted when using carbon addition, at least in the form and concentration of sucrose, as a treatment for reducing non‐native plants on sites where conserving biocrust is a goal. 相似文献