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41.
Comparative assessment of the sensitivity of oilseed rape and wheat to limited water supply 下载免费PDF全文
The drought‐sensitivity of oilseed rape (OSR, Brassica napus cv. SW Landmark) was investigated, using the more widely studied crop species wheat (Triticum aestivum cv. Tybalt) as a benchmark. The water relations of OSR and wheat were compared in lysimeter and controlled environment experiments to test the hypothesis that the growth of OSR is restricted to a greater extent by soil drying than wheat and to determine whether the greater sensitivity results from differences in root or shoot traits. Plants were grown, with or without irrigation, in 1.2 m tall lysimeters packed with a sandy clay loam soil. The experiment was conducted in an open‐sided glasshouse to encourage air flow and to resemble a field environment as far as possible; plant population densities were equivalent to commercial crops. Irrigated OSR (evapo)transpired more water than wheat (498 vs. 355 mm), but had a comparable water use efficiency (WUE; 4.1 vs. 4.4 g DW mm?1 H2O). Oilseed rape showed a greater reduction in above‐ground growth (52% vs. 21%) and a smaller increase in WUE (27% vs. 45%) when water was withheld. Oilseed rape also responded to soil drying at a lower soil moisture deficit than wheat; transpiration rates fell below the potential of irrigated plants when plant available water remaining in the soil profile declined from 54 to 23% compared to 38 to 9% for wheat. The root hydraulic conductivity of young OSR plants, measured on root surface area basis, was about twice that of wheat, and was comparable on a root length basis. The results show that OSR was more sensitive to a restricted water supply than the benchmark species wheat and that the greater sensitivity resulted from differences in shoot, rather than root, characteristics. The root system of OSR was at least as efficient as wheat at extracting water from soil. 相似文献
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Bergman P Linde C Pütsep K Pohanka A Normark S Henriques-Normark B Andersson J Björkhem-Bergman L 《PloS one》2011,6(8):e24394
Background
Statin treatment has been associated with a beneficial outcome on respiratory tract infections. In addition, previous in vitro and in vivo experiments have indicated favorable effects of statins in bacterial infections.Aim
The aim of the present study was to elucidate possible antibacterial effects of statins against primary pathogens of the respiratory tract.Methods
MIC-values for simvastatin, fluvastatin and pravastatin against S. pneumoniae, M. catarrhalis and H. influenzae were determined by traditional antibacterial assays. A BioScreen instrument was used to monitor effects of statins on bacterial growth and to assess possible synergistic effects with penicillin. Bacterial growth in whole blood and serum from healthy volunteers before and after a single dose of simvastatin, fluvastatin and penicillin (positive control) was determined using a blood culture system (BactAlert).Findings
The MIC-value for simvastatin against S pneumoniae and M catarrhalis was 15 µg/mL (36 mmol/L). Fluvastatin and Pravastatin showed no antibacterial effect in concentrations up to 100 µg/mL (230 µmol/L). Statins did not affect growth or viability of H influenzae. Single doses of statins given to healthy volunteers did not affect growth of pneumococci, whereas penicillin efficiently killed all bacteria.Conclusions
Simvastatin at high concentrations 15 µg/mL (36 µmol/L) rapidly kills S pneumoniae and M catarrhalis. However, these concentrations by far exceed the concentrations detected in human blood during simvastatin therapy (1–15 nmol/L) and single doses of statins given to healthy volunteers did not improve antibacterial effects of whole blood. Thus, a direct bactericidal effect of statins in vivo is probably not the mechanism behind the observed beneficial effect of statins against various infections. 相似文献45.
Molecular Ecology Resources Primer Development Consortium Austin JD Bertin A Bórquez JP Cárdenas L Cardoza TB Chapman F De Sousa AC De Souza AP Douglas KC Ellwood SR Ferriol M Garmendia A Gouin N Hargrove J Jasti M Keränen I Knott KE Konec M Kuitunen K Lima MP Linde CC Merle H Oliva ME Pérez M Saarinen E Samollow PB Scarpassa VM Segura I Smith L Trontelj P Valdivia IM Wallwork H Wellenreuther M 《Molecular ecology resources》2011,11(4):757-758
This article documents the addition of 111 microsatellite marker loci to the Molecular Ecology Resources Database. Loci were developed for the following species: Acipenser oxyrinchus desotoi, Anopheles nuneztovari sensu lato, Asellus aquaticus, Calopteryx splendens, Calopteryx virgo, Centaurea aspera, Centaurea seridis, Chilina dombeyana, Proctoeces cf. lintoni and Pyrenophora teres f. teres. 相似文献
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K. Linde 《Archives of microbiology》1959,32(3):234-242
Zusammenfassung Es wird ein gemeinsames Wachstum von ammonstarken monosaccharidspaltenden Darmbakterien mit einem nicht näher definierten gramnegativen Verunreinigungskeim in synthetischer 1%iger Koser-Saccharose-Lösung beschrieben. Da die fortlaufende Kultivierung unbegrenzt gelang, handelte es sich um eine echte Vermehrung, die keinem der beiden Keime in dieser Form als Reinkultur möglich war. Es werden Angaben über verschiedene Stoffwechselleistungen der Reinkulturen bzw. im symbiotischen System gegeben. 相似文献
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The aims of this study were to characterize the hysterographic and histological features of the uteri and to perform immunohistochemistry with proliferating cell nuclear antigen (PCNA) in the cat endometrium at various stages of the reproductive cycle and after treatment with exogenous progestagen. Seventy-four female domestic cats submitted for routine ovariohysterectomy were categorized into six groups: inactive (n=20), follicular (n=9), luteal (n=18), and postpartum (n=12) stages of the reproductive cycle; cats given medroxyprogesterone acetate for estrus prevention (MPA group) (n=12); and cats with uterine pathological lesions (n=3). Hysterography was performed and the relation of the uterine and luminal shape in the hysterogram with the stage of the reproductive cycle as well as with any pathological conditions of the uterus was evaluated. The uteri and ovaries were thereafter surgically removed and sectioned for histological examination. The PCNA was used to demonstrate the expression of endometrial epithelial cell growth. The hysterographic appearance was found to differ between the six groups of cats. A straight uterine cavity was characteristic for cats in the inactive stage, whereas a wavy uterine cavity was characteristic for cats in the follicular stage. In the luteal stage, the luminal cavity of the uteri differed in shape with increasing progesterone concentration from straight to irregular wavy or coiled. The coil shaped uterine lumen seen in the MPA treated and pathological groups was considered also to be an expression of a progestagenic effect. Waviness and coiling of the uterine lumen was related to a proliferation of the endometrial glands, whereas irregular filling defects were indicative of endometrial cystic changes. This study is the first to demonstrate the expression of PCNA in the cat endometrium although no differences were found between the six groups of cats. The hysterographic appearance was found to differ according to stage of the reproductive cycle and pathological conditions. Thus, a normative hysterogram is now available for diagnosing the reproductive stage and uterine changes in cats developing endometrial hyperplasia with and without cystic changes. 相似文献
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Bihl F Frahm N Di Giammarino L Sidney J John M Yusim K Woodberry T Sango K Hewitt HS Henry L Linde CH Chisholm JV Zaman TM Pae E Mallal S Walker BD Sette A Korber BT Heckerman D Brander C 《Journal of immunology (Baltimore, Md. : 1950)》2006,176(7):4094-4101
Immunodominance is variably used to describe either the most frequently detectable response among tested individuals or the strongest response within a single individual, yet factors determining either inter- or intraindividual immunodominance are still poorly understood. More than 90 individuals were tested against 184 HIV- and 92 EBV-derived, previously defined CTL epitopes. The data show that HLA-B-restricted epitopes were significantly more frequently recognized than HLA-A- or HLA-C-restricted epitopes. HLA-B-restricted epitopes also induced responses of higher magnitude than did either HLA-A- or HLA-C-restricted epitopes, although this comparison only reached statistical significance for EBV epitopes. For both viruses, the magnitude and frequency of recognition were correlated with each other, but not with the epitope binding affinity to the restricting HLA allele. The presence or absence of HIV coinfection did not impact EBV epitope immunodominance patterns significantly. Peptide titration studies showed that the magnitude of responses was associated with high functional avidity, requiring low concentration of cognate peptide to respond in in vitro assays. The data support the important role of HLA-B alleles in antiviral immunity and afford a better understanding of the factors contributing to inter- and intraindividual immunodominance. 相似文献
50.
Tuber melanosporum is a truffle native to Europe and is cultivated in countries such as Australia for the gastronomic market, where production yields are often lower than expected. We assessed the genetic diversity of T. melanosporum with six microsatellite loci to assess the effect of genetic drift on truffle yield in Australia. Genetic diversity as assessed on 210 ascocarps revealed a higher allelic diversity compared to previous studies from Europe, suggesting a possible genetic expansion and/or multiple and diverse source populations for inoculum. The results also suggest that the single sequence repeat diversity of locus ME2 is adaptive and that, for example, the probability of replication errors is increased for this locus. Loss of genetic diversity in Australian populations is therefore not a likely factor in limiting ascocarp production. A survey of nursery seedlings and trees inoculated with T. melanosporum revealed that <70% of seedlings and host trees were colonized with T. melanosporum and that some trees had been contaminated by Tuber brumale, presumably during the inoculation process. Mating type (MAT1-1-1 and MAT1-2-1) analyses on seedling and four- to ten-year-old host trees found that 100% of seedlings but only approximately half of host trees had both mating types present. Furthermore, MAT1-1-1 was detected significantly more commonly than MAT1-2-1 in established trees, suggesting a competitive advantage for MAT1-1-1 strains. This study clearly shows that there are more factors involved in ascocarp production than just the presence of both mating types on host trees. 相似文献