全文获取类型
收费全文 | 8276篇 |
免费 | 709篇 |
国内免费 | 3篇 |
出版年
2023年 | 30篇 |
2022年 | 77篇 |
2021年 | 108篇 |
2020年 | 69篇 |
2019年 | 75篇 |
2018年 | 112篇 |
2017年 | 103篇 |
2016年 | 181篇 |
2015年 | 319篇 |
2014年 | 367篇 |
2013年 | 444篇 |
2012年 | 565篇 |
2011年 | 564篇 |
2010年 | 416篇 |
2009年 | 357篇 |
2008年 | 500篇 |
2007年 | 505篇 |
2006年 | 460篇 |
2005年 | 482篇 |
2004年 | 506篇 |
2003年 | 445篇 |
2002年 | 477篇 |
2001年 | 70篇 |
2000年 | 56篇 |
1999年 | 116篇 |
1998年 | 139篇 |
1997年 | 56篇 |
1996年 | 79篇 |
1995年 | 64篇 |
1994年 | 73篇 |
1993年 | 85篇 |
1992年 | 79篇 |
1991年 | 61篇 |
1990年 | 74篇 |
1989年 | 47篇 |
1988年 | 61篇 |
1987年 | 59篇 |
1986年 | 55篇 |
1985年 | 49篇 |
1984年 | 69篇 |
1983年 | 48篇 |
1982年 | 65篇 |
1981年 | 73篇 |
1980年 | 46篇 |
1979年 | 46篇 |
1978年 | 40篇 |
1977年 | 43篇 |
1976年 | 35篇 |
1975年 | 25篇 |
1974年 | 27篇 |
排序方式: 共有8988条查询结果,搜索用时 15 毫秒
821.
822.
823.
824.
825.
826.
The ability to mount a successful response to threats is critical for an organism's survival. A key element of the stress response is its nonspecificity toward the stress source, with similar endocrine and behavioral changes expected under a variety of stressors. In this project we utilized an experimental design that accounts for multiple sources of variation to further understand the nature of stress responsivity and its relationship to the early rearing environment. A sample of baboons (n=73) was observed during the early phase of life in their social group, and then tested as juveniles in a challenging situation. Maternal cortisol levels were measured during the peripartum period. The challenging situation (individuals were isolated for a few minutes in a single cage) was designed to be a moderate source of psychological stress. Patterns in individual differences during the stress test were "mapped" by means of multidimensional scaling (MDS). After the observation was made, the subject was sedated and a blood sample was taken to measure cortisol levels. Our results indicate that when juvenile baboons are confronted with a source of psychological stress, they show a multidimensional behavioral response, probably mediated by the activation and synergic interaction among different neurohormonal systems that, ultimately, act on the hypothalamus-pituitary-adrenal (HPA) axis. Different components of the multidimensional, or nonspecific, behavioral response are associated with the quality and quantity of interactions with their mothers during early life. Juveniles whose mothers displayed higher levels of positive interaction were characterized by vigilant but less active reactions to the stress test, whereas juveniles of mothers that displayed high levels of stress-related behaviors had higher cortisol and locomotion levels. 相似文献
827.
Myc stimulates nuclearly encoded mitochondrial genes and mitochondrial biogenesis 总被引:12,自引:0,他引:12 下载免费PDF全文
Li F Wang Y Zeller KI Potter JJ Wonsey DR O'Donnell KA Kim JW Yustein JT Lee LA Dang CV 《Molecular and cellular biology》2005,25(14):6225-6234
828.
Saba S Janczewski AM Baker LC Shusterman V Gursoy EC Feldman AM Salama G McTiernan CF London B 《American journal of physiology. Heart and circulatory physiology》2005,289(4):H1456-H1467
Transgenic mice overexpressing the inflammatory cytokine TNF-alpha in the heart develop a progressive heart failure syndrome characterized by biventricular dilatation, decreased ejection fraction, decreased survival compared with non-transgenic littermates, and earlier pathology in males. TNF-alpha mice (TNF1.6) develop atrial arrhythmias on ambulatory telemetry monitoring that worsen with age and are more severe in males. We performed in vivo electrophysiological testing in transgenic and control mice, ex vivo optical mapping of voltage in the atria of isolated perfused TNF1.6 hearts, and in vitro studies on isolated atrial muscle and cells to study the mechanisms that lead to the spontaneous arrhythmias. Programmed stimulation induces atrial arrhythmias (n = 8/32) in TNF1.6 but not in control mice (n = 0/37), with a higher inducibility in males. In the isolated perfused hearts, programmed stimulation with single extra beats elicits reentrant atrial arrhythmias (n = 6/6) in TNF1.6 but not control hearts due to slow heterogeneous conduction of the premature beats. Lowering extracellular Ca(2+) normalizes conduction and prevents the arrhythmias. Atrial muscle and cells from TNF1.6 compared with control mice exhibit increased collagen deposition, decreased contractile function, and abnormal systolic and diastolic Ca(2+) handling. Thus abnormalities in action potential propagation and Ca(2+) handling contribute to the initiation of atrial arrhythmias in this mouse model of heart failure. 相似文献
829.
Duncan JG Ravi R Stull LB Murphy AM 《American journal of physiology. Heart and circulatory physiology》2005,289(4):H1512-H1518
Heart failure is a clinical syndrome associated with elevated levels of oxygen-derived free radicals. Xanthine oxidase activity is believed to be one source of reactive oxygen species in the failing heart. Interventions designed to reduce oxidative stress are believed to have significant therapeutic potential in heart failure. This study tested the hypothesis that xanthine oxidase activity would be elevated in a mouse model of dilated cardiomyopathy and evaluated the effect of chronic oral allopurinol, an inhibitor of xanthine oxidase, on contractility and progressive ventricular dilation in these mice. Nontransgenic and transgenic mice containing a troponin I truncation were treated with oral allopurinol from 2-4 mo of age. Myocardial xanthine oxidase activity was threefold higher in untreated transgenic mice compared with nontransgenic mice. Analyses of myofilament proteins for modification of carbonyl groups demonstrated myofibrillar protein damage in untreated transgenic mice. Treatment with allopurinol for 2 mo suppressed xanthine oxidase activity and myofibrillar protein oxidation. Allopurinol treatment also alleviated ventricular dilation and preserved shortening fraction in the transgenic animals. In addition, cardiac muscle twitch tension was preserved to 70% of nontransgenic levels in allopurinol-treated transgenic mice, a significant improvement over untreated transgenic mice. These findings indicate that chronic inhibition of xanthine oxidase can alter the progression of heart failure in dilated cardiomyopathy. 相似文献
830.
The cultivation of exotic Penaeus vannamei in Thailand began on a very limited scale in the late 1990s, but a Thai government ban on the cultivation of P. monodon in freshwater areas in 2000 led many Thai shrimp farmers to shift to cultivation of P. vannamei. Alarmed by the possibility of Taura syndrome virus (TSV) introduction, the Thai Department of Fisheries required that imported stocks of P. vannamei be certified free of TSV by RT-PCR (Reverse Trasciption Polymerase Chain Reaction) testing. During the interval of allowed importation, over 150,000 broodstock shrimp were imported, 67% of these from China and Taiwan. Despite the safeguards, TSV outbreaks occurred and we confirmed the first outbreak by RT-PCR in early 2003. This resulted in a governmental ban on all shrimp broodstock imports from February 2003, but TSV outbreaks have continued, possibly due to original introductions or to the continued illegal importation of stocks. To determine the origin of the TSV in Thailand, the viral coat protein gene VP1 was amplified by RT-PCR from several shrimp specimens found positive for TSV by RT-PCR from January to November 2003. These included 7 samples from P. vannamei disease outbreaks in Thailand, 3 other non-diseased shrimp samples from Thailand and Burma and 6 samples including P. vannamei and P. japonicus from China. Comparison revealed that the Thai, Burmese and Chinese TSV types formed a clade distinct from a clade of TSV types from the Americas. 相似文献