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981.
Xiao Li Xiaobei Zang Xinming Li Miao Zhu Qiao Chen Kunlin Wang Minlin Zhong Jinquan Wei Dehai Wu Hongwei Zhu 《Liver Transplantation》2014,4(14)
A hybrid heterojunction and solid‐state photoelectrochemical solar cell based on graphene woven fabrics (GWFs) and silicon is designed and fabricated. The GWFs are transferred onto n‐Si to form a Schottky junction with an embedded polyvinyl alcohol based solid electrolyte. In the hybrid solar cell, solid electrolyte serves three purposes simutaneously; it is an anti‐reflection layer, a chemical modification carrier, and a photoelectrochemical channel. The open‐circuit voltage, short‐circuit current density, and fill factor are all significantly improved, achieving an impressive power conversion efficiency of 11%. Solar cell models are constructed to confirm the hybrid working mechanism, with the heterojunction junction and photoelectrochemical effect functioning synergistically. 相似文献
982.
Aphids harbor primary endosymbionts, Buchnera aphidicola, in specialized cells within their body cavities. Aphids and Buchnera have strict mutualistic relationships in nutrition exchange. This ancient association has received much attention from researchers who are interested in endosymbiotic evolution. Previous studies have found parallel phylogenetic relationships between non‐galling aphids and Buchnera at lower taxonomic levels (genus, species). To understand whether relatively isolated habitats such as galls have effect on the parallel relationships between aphids and Buchnera, the present paper investigated the phylogenetic relationships of gall aphids from Pemphigus and allied genera, which induce pseudo‐galls or galls on Populus spp. (poplar) and Buchnera. The molecular phylogenies inferred from three aphid genes (COI, COII and EF‐1α) and two Buchnera genes (gnd, 16S rRNA gene) indicated significant congruence between aphids and Buchnera at generic as well as interspecific levels. Interestingly, both aphid and Buchnera phylogenies supported three main clades corresponding to the galling locations of aphids, namely leaf, the joint of leaf blade and petiole, and branch of the host plant. The results suggest phylogenetic conservatism of gall characters, which indicates gall characters are more strongly affected by aphid phylogeny, rather than host plants. 相似文献
983.
LiYing Yan Yuan Wei Jin Huang XiaoHui Zhu XiaoDan Shi Xi Xia Jie Yan CuiLing Lu Ying Lian Rong Li Ping Liu Jie Qiao 《中国科学:生命科学英文版》2014,57(7):665-671
Preimplantation genetic diagnosis (PGD) gives couples who have a high risk of transmitting genetic disorders to their baby the chance to have a healthy offspring through embryo genetic analysis and selection. Preimplantation genetic screening (PGS) is an effective method to select euploid embryos that may prevent repeated implantation failure or miscarriage. However, how and to whom PGS should be provided is a controversial topic. The first successful case of PGD of a human being was reported in 1990, and there have been tremendous improvements in this technology since then. Both embryo biopsy and genetic technologies have been improved dramatically, which increase the accuracy and expand the indications of PGD/PGS. 相似文献
984.
Alexander W Wyatt Fan Mo Kendric Wang Brian McConeghy Sonal Brahmbhatt Lina Jong Devon M Mitchell Rebecca L Johnston Anne Haegert Estelle Li Janet Liew Jake Yeung Raunak Shrestha Anna V Lapuk Andrew McPherson Robert Shukin Robert H Bell Shawn Anderson Jennifer Bishop Antonio Hurtado-Coll Hong Xiao Arul M Chinnaiyan Rohit Mehra Dong Lin Yuzhuo Wang Ladan Fazli Martin E Gleave Stanislav V Volik Colin C Collins 《Genome biology》2014,15(8)
985.
Christian B Matranga Kristian G Andersen Sarah Winnicki Michele Busby Adrianne D Gladden Ryan Tewhey Matthew Stremlau Aaron Berlin Stephen K Gire Eleina England Lina M Moses Tarjei S Mikkelsen Ikponmwonsa Odia Philomena E Ehiane Onikepe Folarin Augustine Goba S Humarr Kahn Donald S Grant Anna Honko Lisa Hensley Christian Happi Robert F Garry Christine M Malboeuf Bruce W Birren Andreas Gnirke Joshua Z Levin Pardis C Sabeti 《Genome biology》2014,15(11)
We have developed a robust RNA sequencing method for generating complete de novo assemblies with intra-host variant calls of Lassa and Ebola virus genomes in clinical and biological samples. Our method uses targeted RNase H-based digestion to remove contaminating poly(rA) carrier and ribosomal RNA. This depletion step improves both the quality of data and quantity of informative reads in unbiased total RNA sequencing libraries. We have also developed a hybrid-selection protocol to further enrich the viral content of sequencing libraries. These protocols have enabled rapid deep sequencing of both Lassa and Ebola virus and are broadly applicable to other viral genomics studies.
Electronic supplementary material
The online version of this article (doi:10.1186/s13059-014-0519-7) contains supplementary material, which is available to authorized users. 相似文献986.
Yang Ning Huang Bin Tsinkalovsky Oleg Brekkå Narve Zhu Huaiyang Leiss Lina Enger Per Øyvind Li Xingang Wang Jian 《Cancer cell international》2014,14(1):1-7
The anticancer effects of ceramide have been reported in many types of cancers but less in lung cancer. In this study, we used C2-ceramide to further investigate its possible anticancer effects and mechanisms on non-small cell lung cancer (NSCLC) H1299 cells. The result of cell proliferation in terms of trypan blue assay showed high dose of C2-ceramide inhibited cell survival after 24 h treatment. The flow cytometry-based assays indicated the effect of apoptosis, chromatin condensation, and G1 arrest in terms of Annexin V/propidium iodide (PI), DAPI, and PI stainings, respectively. Moreover, the decreased protein level of p-Akt, p-NFκB, survivin and cyclin A2 were detected by Western blot assay. Taken together, these results indicated the antiproliferative effect of C2-ceramide is majorly responsible for cell apoptosis in lung cancer H1299 cells. 相似文献
987.
Katharigatta N. Venugopala Sandeep Chandrashekharappa Pran Kishore Deb Christophe Tratrat Melendhran Pillay Deepak Chopra Nizar A. Al-Shari Wafa Hourani Lina A. Dahabiyeh Pobitra Borah Rahul D. Nagdeve Susanta K. Nayak Basavaraj Padmashali Mohamed A. Morsy Bandar E. Aldhubiab Mahesh Attimarad Anroop B. Nair Nagaraja Sreeharsha Michelyne Haroun Sheena Shashikanth Viresh Mohanlall Raghuprasad Mailavaram 《Journal of enzyme inhibition and medicinal chemistry》2021,36(1):1472
A series of 1,2,3-trisubstituted indolizines (2a–2f, 3a–3d, and 4a–4c) were screened for in vitro whole-cell anti-tubercular activity against the susceptible H37Rv and multidrug-resistant (MDR) Mycobacterium tuberculosis (MTB) strains. Compounds 2b–2d, 3a–3d, and 4a–4c were active against the H37Rv-MTB strain with minimum inhibitory concentration (MIC) ranging from 4 to 32 µg/mL, whereas the indolizines 4a–4c, with ethyl ester group at the 4-position of the benzoyl ring also exhibited anti-MDR-MTB activity (MIC = 16–64 µg/mL). In silico docking study revealed the enoyl-acyl carrier protein reductase (InhA) and anthranilate phosphoribosyltransferase as potential molecular targets for the indolizines. The X-ray diffraction analysis of the compound 4b was also carried out. Further, a safety study (in silico and in vitro) demonstrated no toxicity for these compounds. Thus, the indolizines warrant further development and may represent a novel promising class of InhA inhibitors and multi-targeting agents to combat drug-sensitive and drug-resistant MTB strains. 相似文献
988.
Qiao Lu Meixing Yu Chongyang Shen Xiaoping Chen Ting Feng Yongchao Yao Jinrong Li Hong Li Wenwei Tu 《PloS one》2014,9(12)
Human induced pluripotent stem cells (hiPSCs) have potential applications in cell replacement therapy and regenerative medicine. However, limited information is available regarding the immunologic features of iPSCs. In this study, expression of MHC and T cell co-stimulatory molecules in hiPSCs, and the effects on activation, proliferation and cytokine production in allogeneic human peripheral blood mononuclear cells were examined. We found that no-integrate hiPSCs had no MHC-II and T cell co-stimulatory molecules expressions but had moderate level of MHC-I and HLA-G expressions. In contrast to human skin fibroblasts (HSFs) which significantly induced allogeneic T cell activation and proliferation, hiPSCs failed to induce allogeneic CD45+ lymphocyte and CD8+ T cell activation and proliferation but could induce a low level of allogeneic CD4+ T cell proliferation. Unlike HSFs which induced allogeneic lymphocytes to produce high levels of IFN-γ, TNF-α and IL-17, hiPSCs only induced allogeneic lymphocytes to produce IL-2 and IL-10, and promote IL-10-secreting regulatory T cell (Treg) generation. Our study suggests that the integration-free hiPSCs had low or negligible immunogenicity, which may result from their induction of IL-10-secreting Treg. 相似文献
989.
Qinggang Wang Dachuan Bao Yili Guo Junmeng Lu Zhijun Lu Yaozhan Xu Kuihan Zhang Haibo Liu Hongjie Meng Mingxi Jiang Xiujuan Qiao Handong Huang 《PloS one》2014,9(5)
The stochastic dilution hypothesis has been proposed to explain species coexistence in species-rich communities. The relative importance of the stochastic dilution effects with respect to other effects such as competition and habitat filtering required to be tested. In this study, using data from a 25-ha species-rich subtropical forest plot with a strong topographic structure at Badagongshan in central China, we analyzed overall species associations and fine-scale species interactions between 2,550 species pairs. The result showed that: (1) the proportion of segregation in overall species association analysis at 2 m neighborhood in this plot followed the prediction of the stochastic dilution hypothesis that segregations should decrease with species richness but that at 10 m neighborhood was higher than the prediction. (2) The proportion of no association type was lower than the expectation of stochastic dilution hypothesis. (3) Fine-scale species interaction analyses using Heterogeneous Poisson processes as null models revealed a high proportion (47%) of significant species effects. However, the assumption of separation of scale of this method was not fully met in this plot with a strong fine-scale topographic structure. We also found that for species within the same families, fine-scale positive species interactions occurred more frequently and negative ones occurred less frequently than expected by chance. These results suggested effects of environmental filtering other than species interaction in this forest. (4) We also found that arbor species showed a much higher proportion of significant fine-scale species interactions (66%) than shrub species (18%). We concluded that the stochastic dilution hypothesis only be partly supported and environmental filtering left discernible spatial signals in the spatial associations between species in this species-rich subtropical forest with a strong topographic structure. 相似文献
990.
Zi-Yi Jin Ming Wu Ren-Qiang Han Xiao-Feng Zhang Xu-Shan Wang Ai-Ming Liu Jin-Yi Zhou Qing-Yi Lu Claire H. Kim Lina Mu Zuo-Feng Zhang Jin-Kou Zhao 《PloS one》2014,9(7)