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991.
992.
Characteristics of γ-hexachlorocyclohexane biodegradation by a nitrogen-fixing cyanobacterium, Anabaena azotica 总被引:1,自引:0,他引:1
Hangjun Zhang Ciming Hu Xiuying Jia Yi Xu Chenjie Wu Lina Chen Fengping Wang 《Journal of applied phycology》2012,24(2):221-225
Biodegradation of γ-hexachlorocyclohexane (lindane) by a nitrogen-fixing cyanobacterium isolated from Chinese paddy soils,
Anabaena azotica 118, was investigated. Lindane with an initial concentration of 0.2 mg L−1 in the cultures had no negative effect on the chlorophyll a concentration of A. azotica after 5 d exposure. The tolerance of this cyanobacterium to lindane indicates that it has the potential to biodegrade lindane.
The degradation experiments show that the percentage of lindane removal efficiency by A. azotica was 48.8% after 5 d, at an initial lindane concentration of 0.2 mg L−1 and initial A. azotica chlorophyll a concentration of 50 mg L−1. The calculated half-life was 4.78 d. Elevated culture temperature, irradiation, and usage of nitrate as the nitrogen source
in the cultures could increase the biodegradation efficiency of lindane. γ-Pentachlorocyclohexene was detected as a metabolite
of lindane. The ability of A. azotica to biodegrade lindane has potential use in the bioremediation for this organochlorine pesticide. 相似文献
993.
Effect of prey feeding (ants Formica fusca) on the quantitative changes in the accumulation of free amino acids, soluble proteins, phenolic metabolites and mineral
nutrients in the leaves of carnivorous plant Drosera capensis was studied. Arginine was the most abundant compound in Drosera leaves, while proline was abundant in ants. The amount of the majority of amino acids and their sum were elevated in the
fed leaves after 3 and 21 days, and the same, but with further enhancement after 21 days, was observed in ants. Accumulation
of amino acids also increased in young non-fed leaves of fed plants. Soluble proteins decreased in ants, but were not enhanced
in fed leaves. This confirms the effectiveness of sundew’s enzymatic machinery in digestion of prey and suggests that amino
acids are not in situ deposited, but rather are allocated within the plant. The content of total soluble phenols, flavonoids
and two selected flavonols (quercetin and kaempferol) was not affected by feeding in Drosera leaves, indicating that their high basal level was sufficient for the plant’s metabolism and prey-induced changes were mainly
N based. The prey also showed to be an important source of other nutrients besides N, and a stimulation of root uptake of
some mineral nutrients is assumed (Mg, Cu, Zn). Accumulation of Ca and Na was not affected by feeding. 相似文献
994.
Kandiba L Aitio O Helin J Guan Z Permi P Bamford DH Eichler J Roine E 《Molecular microbiology》2012,84(3):578-593
VP4, the major structural protein of the haloarchaeal pleomorphic virus, HRPV‐1, is glycosylated. To define the glycan structure attached to this protein, oligosaccharides released by β‐elimination were analysed by mass spectrometry and nuclear magnetic resonance spectroscopy. Such analyses showed that the major VP4‐derived glycan is a pentasaccharide comprising glucose, glucuronic acid, mannose, sulphated glucuronic acid and a terminal 5‐N‐formyl‐legionaminic acid residue. This is the first observation of legionaminic acid, a sialic acid‐like sugar, in an archaeal‐derived glycan structure. The importance of this residue for viral infection was demonstrated upon incubation with N‐acetylneuraminic acid, a similar monosaccharide. Such treatment reduced progeny virus production by half 4 h post infection. LC‐ESI/MS analysis confirmed the presence of pentasaccharide precursors on two different VP4‐derived peptides bearing the N‐glycosylation signal, NTT. The same sites modified by the native host, Halorubrum sp. strain PV6, were also recognized by the Haloferax volcanii N‐glycosylation apparatus, as determined by LC‐ESI/MS of heterologously expressed VP4. Here, however, the N‐linked pentasaccharide was the same as shown to decorate the S‐layer glycoprotein in this species. Hence, N‐glycosylation of the haloarchaeal viral protein, VP4, is host‐specific. These results thus present additional examples of archaeal N‐glycosylation diversity and show the ability of Archaea to modify heterologously expressed proteins. 相似文献
995.
Fang Du Zhong-ming Qian Qi Gong Zhou Jin Zhu Lina Lu Ya Ke 《The Journal of nutritional biochemistry》2012,23(12):1694-1700
The mechanism by which hepcidin controls cellular iron release protein ferroportin 1 (Fpn1) in macrophages has been well established. However, little is known about the effects of hepcidin on cellular iron uptake proteins. Here, we demonstrated for the first time that hepcidin can significantly inhibit the expression of transferrin receptor 1 (TfR1) and divalent metal transporter 1 in addition to Fpn1, and therefore reduce transferrin-bound iron and non-transferrin-bound iron uptake and also iron release in J774 macrophages. Analysis of mechanisms using the iron-depleted cells showed that hepcidin has a direct inhibitory effect on all iron transport proteins we examined. Further studies demonstrated that the down-regulation of TfR1 induced by hepcidin is associated with cyclic adenosine monophosphate (cAMP) and protein kinase A (PKA), probably being mediated by the cAMP–PKA pathway in J774 macrophages. 相似文献
996.
Fungal species are a very important source of many different enzymes, and the ability of fungi to transform steroids has been used for several decades in the production of compounds with a sterane skeleton. Here, we review the characterised and/or purified enzymes for steroid transformations, dividing them into two groups: (i) enzymes of the ergosterol biosynthetic pathway, including data for, e.g. ERG11 (14α-demethylase), ERG6 (C-24 methyltransferase), ERG5 (C-22 desaturase) and ERG4 (C-24 reductase); and (ii) the other steroid-transforming enzymes, including different hydroxylases (7α-, 11α-, 11β-, 14α-hydroxylase), oxidoreductases (5α-reductase, 3β-hydroxysteroid dehydrogenase/isomerase, 17β-hydroxysteroid dehydrogenase, C-1/C-2 dehydrogenase) and C-17-C-20 lyase. The substrate specificities of these enzymes, their cellular localisation, their association with protein super-families, and their potential applications are discussed. Article from a special issue on steroids and microorganisms. 相似文献
997.
Beranič N Brožič P Brus B Sosič I Gobec S Lanišnik Rižner T 《The Journal of steroid biochemistry and molecular biology》2012,130(1-2):16-25
The human aldo-keto reductase AKR1C2 converts 5α-dihydrotestosterone to the less active 3α-androstanediol and has a minor 20-ketosteroid reductase activity that metabolises progesterone to 20α-hydroxyprogesterone. AKR1C2 is expressed in different peripheral tissues, but its role in uterine diseases like endometriosis has not been studied in detail. Some progestins used for treatment of endometriosis inhibit AKR1C1 and AKR1C3, with unknown effects on AKR1C2. In this study we investigated expression of AKR1C2 in the model cell lines of peritoneal endometriosis, and examined the ability of recombinant AKR1C2 to metabolise progesterone and progestin dydrogesterone, as well as its potential inhibition by progestins. AKR1C2 is expressed in epithelial and stromal endometriotic cell lines at the mRNA level. The recombinant enzyme catalyses reduction of progesterone to 20α-hydroxyprogesterone with a 10-fold lower catalytic efficiency than the major 20-ketosteroid reductase, AKR1C1. AKR1C2 also metabolises progestin dydrogesterone to its 20α-dihydrodydrogesterone, with 8.6-fold higher catalytic efficiency than 5α-dihydrotestosterone. Among the progestins that are currently used for treatment of endometriosis, dydrogesterone, medroxyprogesterone acetate and 20α-dihydrodydrogesterone act as AKR1C2 inhibitors with low μM K(i) values in vitro. Their potential in vivo effects should be further studied. 相似文献
998.
Pospíšilová H Vaňková M Hill M Meloun M Bendlová B Dušková M Stárka L 《The Journal of steroid biochemistry and molecular biology》2012,132(1-2):105-111
The relationships between the parameters of metabolic syndrome and non-aromatizable metabolites of testosterone have been discussed in literature. Some papers describe these metabolites as one of the possible causes of male-type obesity. On the contrary, other studies show a protective influence of dihydrotestosterone on visceral obesity. The aim of this study to analyse the relationship between anthropometric parameters, lipid spectrum, glycemia and the level of endogenous testosterone and dihydrotestosterone, and to compare the effects of these androgens. Our population-based study involved 232 healthy men ranging from 20 to 78 years with BMI 18 to 39 kg/m(2). Serum testosterone, dihydrotestosterone and sex hormone binding globulin SHBG levels, lipid spectrum, glucose metabolism parameters were measured and the oral glucose tolerance test was carried out in all subjects. Their anthropometric parameters (weight, height, waist, hips, waist-to-hip ratio, 14 skin folds) and body composition parameters were determined and calculated by the Antropo program. Multiple regression analysis showed a correlation between hormonal levels, esp. of testosterone and dihydrotestosterone, and the anthropometric data, lipid spectrum and parameters of glucose regulation. Low testosterone and/or dihydrotestosterone was correlated to a higher body-mass index, fat content, waist diameter, total-, HDL-, LDL-cholesterol and triglycerides, fasting glucose, insulin resistance and lower muscle and bone mass. In addition, statistical analysis using multivariate regression with reduction in dimensionality did not discover any striking difference between aromatizable and non-aromatizable androgens in their association to lipid and glucose metabolism parameters in healthy, normosthenic men. In conclusion, the association of endogenous testosterone and dihydrotestosterone to anthropometric data, lipid spectrum and insulin sensitivity are of the same quality; however, the effect of the circulating levels of dihydrotestosterone is quantitatively smaller. 相似文献
999.
Species living in open sandy habitats are declining in northern Europe because of habitat loss and degradation. However, mining
of sand creates potential new habitats for these species in the form of sand pits. In this study we investigated the beetle
fauna in sand pits in order to determine what kind of sand pits are the most valuable for conservation, in terms of sand pit
area (primarily) and the proportion of sand material, vegetation cover, tree cover and edge habitats. Thirteen sand pits in
Uppsala County, Sweden, ranging in size from 0.02 to 18 ha, were included in this study. A total of 2,500 individuals of beetles,
belonging to 256 species, were sampled by pitfall traps. Thirty-nine of the species were classified as specialized sand-dwelling
species and two were Swedish Red List species. We found that the area of sand pits affects both species number and species
composition of beetles. A positive species-area relationship was found, best explained by a quadratic power function, for
the habitat-specific species (i.e., sand species). Our recommendation is to prioritize sand pits with areas between 0.3 and
5 ha, with preference towards the lower end of this range, for conserving sand-dwelling beetles. 相似文献
1000.
Improved volatile fatty acids production from proteins of sewage sludge with anthraquinone-2,6-disulfonate (AQDS) under anaerobic condition 总被引:1,自引:0,他引:1
Organic matters in sewage sludge can be converted into volatile fatty acids (VFAs) as renewable carbon sources. This work for the first time applied anthraquinone-2,6-disulfonate (AQDS) for enhancing VFA production from sewage sludge. With 0.066 or 0.33 g AQDS g−1 dried solids (DS), the yields for VFAs peak at 403 or 563 mg l−1, 1.9- or 2.7-fold to the control. The accumulated VFAs were principally composed of acetate and propionate. The AQDS enhances degradation rates of model proteins (bovine serum albumin), but had little enhancement on that of model polysaccharides (dextrans). The acidification step is proposed the rate-limiting step for VFA production from sewage sludge, in which the AQDS molecules shuttle electrons to accelerate the redox reactions associated with amino acid degradation. Methanogenic activities are inhibited in the presence of AQDS. The AQDS-assisted VFAs are renewable organic carbon sources, although their direct use for anaerobic digestion is not advised. 相似文献