Sustainability of SARS-CoV-2 Induced Humoral Immune Responses in COVID-19 Patients from Hospitalization to Convalescence Over Six Months

Virologica Sinica - Understanding the persistence of antibody in convalescent COVID-19 patients may help to answer the current major concerns such as the risk of reinfection, the protection period...     

Genetic Analysis of Human Adenovirus Type 7 Strains Circulating in Different Parts of China

To investigate the molecular epidemiology and genetic variation of human adenovirus type 7(HAdV-7) in children with acute respiratory infections(ARI) in China. HAdV-7-positive respiratory samples collected from children with ARI in Beijing, Shijiazhuang, Wenzhou and Guangzhou from 2014–2018 were selected for gene amplification and sequence analysis. Fifty-seven HAdV-7 clinical strains with hexon, penton base and fiber gene sequences were obtained. Meanwhile17 strains were selected randomly from different cities for whole genome sequencing. Phylogenetic and variation analyses were performed based on the obtained sequences, HAdV-7 prototype strain Gomen(AY594255), vaccine strains(AY495969 and AY594256) and representative sequences of strains. The phylogenetic trees constructed based on whole genome sequences, major capsid protein genes(hexon, penton base and fiber) and the early genes(E1, E2, E3 and E4) were not completely consistent. The HAdV-7 strains obtained in this study always clustered with most of the circulating strains worldwide from the 1980 s to the present. Compared with the HAdV-7 prototype strain Gomen(AY594255), some amino acid mutations in loop1 and loop2 of hexon and the RGD loop region of the penton base gene were observed. Recombination analysis showed that partial regions of 55 k Da protein and 100 kDa hexon-assembly associated protein genes among all HAdV-7 strains in this study were from HAdV-16 and HAdV-3, respectively. Our study demonstrated the molecular evolution characteristics of HAdV-7 strains circulating in China and provided basic reference data for the prevention, control and vaccine development of HAdV-7.     

A Multicenter Study of Viral Aetiology of Community-Acquired Pneumonia in Hospitalized Children in Chinese Mainland

Virologica Sinica - Community-acquired pneumonia (CAP) is one of the leading causes of morbidity and mortality in children worldwide. In this study, we aimed to describe the aetiology of viral...     

Generation and Characterization of a Nanobody Against SARS-CoV

Virologica Sinica - The sudden emergence of severe acute respiratory syndrome coronavirus (SARS-CoV) has caused global panic in 2003, and the risk of SARS-CoV outbreak still exists. However, no...     

广西麻鸡FOXL2基因的克隆、序列分析及在高低产蛋量卵巢中的表达

为了研究鸡FOXL2基因的结构和功能,本研究克隆了广西麻鸡FOXL2基因编码区序列,分析了其突变位点及与其他物种的同源性和进化距离,以及在高产组和低产组母鸡卵巢组织中的表达水平.结果 表明:广西麻鸡FOXL2基因的编码区长度为918 bp,共编码305个氨基酸,存在一处错义突变和两处同义突变.通过物种间的同源性分析显示,广西麻鸡FOXL2基因与原鸡(Gallus gallus)、雉鸡(Phasianus colchicus)、绿头鸭(Anas platyrhynchos)、野鸽(Columba livia)、人(Homo sapiens)、小鼠(Mus musculus)的同源性分别为99.7％、98.8％、95.1％、92.0％、75.7％、75.5％.通过种间进化树分析表明,广西麻鸡与原鸡(Gallus gallus)的亲缘关系最近,与小鼠(Mus musculus)的亲缘关系最远.FOXL2在高低产蛋量鸡卵巢中的表达水平结果显示:FOXL2基因在高产蛋组中的表达量显著高于低产蛋组中的表达量(P＜0.05).该研究结果提示鸡FOXL2的序列相对较保守,对卵巢功能的维持和提高产蛋量具有功能性作用.     

鳖甲煎丸通过LncRNA-mRNA顺式调控抑制肝癌细胞

为了研究鳖甲煎丸抑制肝癌细胞过程中lncRNA表达谱的变化及lncRNA-mRNA顺式调控在其中的机制,本研究通过CCK8方法检测鳖甲煎丸作用于SMMC-7721细胞后的增殖活性变化,经全细胞转录组学分析lncRNA的表达和特征后构建LncRNA-mRNA顺式调控网络.结果表明鳖甲煎丸含药血清作用肝癌细胞株后其增殖活性受到抑制.全细胞转录组学分析找到差异表达的172个LncRNA.构建lncRNA-mRNA顺式调控网络,对调控基因进行生存分析发现,SCRN1、PLEKHA1、HNRNPM、TMEM107、ETNK1、ISG20L2的表达与肝细胞癌患者生存率显著负相关(P＜0.05).因此得出结论,LncRNA参与鳖甲煎丸抑制肝癌细胞增殖的过程,LncRNA-mRNA顺式调控是其分子机制之一.