全文获取类型
收费全文 | 84143篇 |
免费 | 6122篇 |
国内免费 | 5000篇 |
专业分类
95265篇 |
出版年
2024年 | 166篇 |
2023年 | 1093篇 |
2022年 | 2487篇 |
2021年 | 4226篇 |
2020年 | 2713篇 |
2019年 | 3371篇 |
2018年 | 3341篇 |
2017年 | 2454篇 |
2016年 | 3514篇 |
2015年 | 5224篇 |
2014年 | 6000篇 |
2013年 | 6504篇 |
2012年 | 7762篇 |
2011年 | 6872篇 |
2010年 | 4154篇 |
2009年 | 3739篇 |
2008年 | 4291篇 |
2007年 | 3836篇 |
2006年 | 3379篇 |
2005年 | 2762篇 |
2004年 | 2363篇 |
2003年 | 2023篇 |
2002年 | 1755篇 |
2001年 | 1289篇 |
2000年 | 1267篇 |
1999年 | 1184篇 |
1998年 | 739篇 |
1997年 | 719篇 |
1996年 | 725篇 |
1995年 | 661篇 |
1994年 | 577篇 |
1993年 | 427篇 |
1992年 | 595篇 |
1991年 | 456篇 |
1990年 | 420篇 |
1989年 | 306篇 |
1988年 | 259篇 |
1987年 | 251篇 |
1986年 | 183篇 |
1985年 | 206篇 |
1984年 | 126篇 |
1983年 | 144篇 |
1982年 | 86篇 |
1981年 | 74篇 |
1980年 | 49篇 |
1979年 | 65篇 |
1977年 | 41篇 |
1976年 | 39篇 |
1974年 | 45篇 |
1973年 | 38篇 |
排序方式: 共有10000条查询结果,搜索用时 0 毫秒
31.
32.
33.
Many animals exhibit different behaviors in different seasons. The photoperiod can have effects on migration, breeding, fur growth, and other processes. The cyclic growth of the fur and feathers of some species of mammals and birds, respectively, is stimulated by the photoperiod as a result of hormone-dependent regulation of the nervous system. To further examine this phenomenon, we evaluated the Arbas Cashmere goat (Capra hircus), a species that is often used in this type of research. The goats were exposed to an experimentally controlled short photoperiod to study the regulation of cyclic cashmere growth. Exposure to a short photoperiod extended the anagen phase of the Cashmere goat hair follicle to increase cashmere production. Assessments of tissue sections indicated that the short photoperiod significantly induced cashmere growth. This conclusion was supported by a comparison of the differences in gene expression between the short photoperiod and natural conditions using gene chip technology. Using the gene chip data, we identified genes that showed altered expression under the short photoperiod compared to natural conditions, and these genes were found to be involved in the biological processes of hair follicle growth, structural composition of the hair follicle, and the morphogenesis of the surrounding skin appendages. Knowledge about differences in the expression of these genes as well as their functions and periodic regulation patterns increases our understanding of Cashmere goat hair follicle growth. This study also provides preliminary data that may be useful for the development of an artificial method to improve cashmere production by controlling the light cycle, which has practical significance for livestock breeding. 相似文献
34.
35.
Substance P-like immunoreactive nerve fibers in the pars distalis of the anterior pituitary in the dog 总被引:1,自引:0,他引:1
Summary The pars distalis of the anterior pituitary is known to be regulated by hypothalamic hormones. Recently, we have discovered the presence of substance P-like immunoreactive nerve fibers in the pars distalis of the monkeys. Substance P-like immunoreactivity in the pars distalis of the dog was investigated in this study. A substantial amount of substance P-like immunoreactive nerve fibers with a large amount of varicosities were found. They were widely distributed in the gland, more abundant along its periphery. Most of them were closely related to the glandular tissue, some were located on vascular walls. Substance P-like immunoreactive nerve fibers were also found in the meningeal sheath of the anterior pituitary. They could be followed into the parenchyma of the gland. 相似文献
36.
37.
Wang F Liu P Zhang Q Zhu J Chen T Arimura SI Tsutsumi N Lin J 《The Plant journal : for cell and molecular biology》2012,72(1):43-56
The balance between mitochondrial fission and fusion is disrupted during mitosis, but the mechanism governing this phenomenon in plant cells remains enigmatic. Here, we used mitochondrial matrix‐localized Kaede protein (mt‐Kaede) to analyze the dynamics of mitochondrial fission in BY‐2 suspension cells. Analysis of the photoactivatable fluorescence of mt‐Kaede suggested that the fission process is dominant during mitosis. This finding was confirmed by an electron microscopic analysis of the size distribution of mitochondria in BY‐2 suspension cells at various stages. Cellular proteins interacting with Myc‐tagged dynamin‐related protein 3A/3B (AtDRP3A and AtDRP3B) were immunoprecipitated with anti‐Myc antibody‐conjugated beads and subsequently identified by microcapillary liquid chromatography–quadrupole time‐of‐flight mass spectrometry (CapLC Q‐TOF) MS/MS. The identified proteins were broadly associated with cytoskeletal (microtubular), phosphorylation, or ubiquitination functions. Mitotic phosphorylation of AtDRP3A/AtDRP3B and mitochondrial fission at metaphase were inhibited by treatment of the cells with a CdkB/cyclin B inhibitor or a serine/threonine protein kinase inhibitor. The fate of AtDRP3A/3B during the cell cycle was followed by time‐lapse imaging of the fluorescence of Dendra2‐tagged AtDRP3A/3B after green‐to‐red photoconversion; this experiment showed that AtDRP3A/3B is partially degraded during interphase. Additionally, we found that microtubules are involved in mitochondrial fission during mitosis, and that mitochondria movement to daughter cell was limited as early as metaphase. Taken together, these findings suggest that mitotic phosphorylation of AtDRP3A/3B promotes mitochondrial fission during plant cell mitosis, and that AtDRP3A/3B is partially degraded at interphase, providing mechanistic insight into the mitochondrial morphological changes associated with cell‐cycle transitions in BY‐2 suspension cells. 相似文献
38.
Jiao-Qi Wang Jin-Ting He Zhen-Wu Du Zong-Shu Li Yong-Feng Liu Jing Mang Zhong-Xin Xu 《Neurochemical research》2013,38(5):961-971
Ischemic stroke is a major composition of cerebrovascular disease, seriously threatening to human health in the world. Activin A (ActA), belonging to transforming growth factor-beta (TGF-β) super family, plays an important role in the hypoxic-ischemic brain injury through ActA/Smads pathway. While as an essential phosphorylation assistor in TGF-β signaling, the functions and mechanisms of smad anchor for receptor activation (SARA) in ischemic brain injury remain poorly understood. To solve this problem and explore the pathological processes of ischemic stroke, we used an Oxygen–Glucose deprivation (OGD) model in nerve growth factor-induced differentiated rattus PC12 pheochromocytoma cells and down regulated the expressions of SARA by RNA interference technology. Our results showed that the repression of SARA before OGD exposure reduced the expressions of Smad2, 3, 4 mRNA and the phosphorylation rate of Smad2 protein, but it did not affect the mRNA expressions of Smad7. After OGD treatment, ActA/Smads pathway was activated and the expression of SARA in the SARA pre-repression group was significantly up-regulated. The pre-repression of SARA increased the sensitivities of nerve-like cells to OGD damage. Moreover, the mRNA expression of Smad7 which was supposed to participate in the negative feedback of ActA/Smads pathway was also elevated due to OGD injury. Taken together, these results suggest a positive role of SARA in assisting the phosphorylation of Smad2 and maintaining the neuron protective effect of ActA/Smads pathway. 相似文献
39.
40.