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Lilly E. L.; Kulis D. M.; Gentien P.; Anderson D. M. 《Journal of plankton research》2002,24(5):443-452
In 1998, the toxins responsible for paralytic shellfish poisoning(PSP) were detected in Thau Lagoon, France. The causative organismwas identified as Alexandrium tamarense, a member of the tamarensisspecies complex. This dinoflagellate was first observed in thelagoon in 1995 by a monitoring programme following more thana decade with no observations of this species. The species isthus new to these waters, but its origins were unknown. In thispaper, morphological and molecular data are analysed for twoclonal cultures established from the 1998 bloom. These dataare compared to results from Alexandrium isolates originatingelsewhere in the world to infer an origin. Thecal plate morphology,restriction fragment length polymorphism, DNA sequencing andtoxin analyses demonstrate that the Thau Lagoon strains areA. catenella, and are closely related to populations of A. catenellafound in temperate Asia, specifically the Japanese TemperateAsian ribotype of the tamarense/catenella/fundyense speciescomplex. They show no homology with strains from western Europeanwaters, including the Mediterranean. Until now, the JapaneseTemperate Asian ribotype has not been reported outside the westernPacific. The most likely scenario is that A. catenella was introducedto Thau Lagoon via the ballast water of a ship docked at Sète,France, a shipping port in direct communication with the lagoon.This case provides a clear example of the dispersal of a toxicAlexandrium species, probably via human activities. 相似文献
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Meredith A. Lilly Natalie A. Kulkulka Paula R. Firmiss Michael J. Ross Andrew S. Flum Grace B. Delos Santos Diana K. Bowen Robert W. Dettman Edward M. Gong 《PloS one》2015,10(11)
Bladder fibrosis is an undesired end point of injury of obstruction and often renders the smooth muscle layer noncompliant. In many cases, the long-term effect of bladder fibrosis is renal failure. Despite our understanding of the progression of this disease, little is known about the cellular mechanisms that lead to a remodeled bladder wall. Resident stem (progenitor) cells have been identified in various organs such as the brain, heart and lung. These cells function normally during organ homeostasis, but become dysregulated after organ injury. Here, we aimed to characterize a mesenchymal progenitor cell population as a first step in understanding its role in bladder fibrosis. Using fluorescence activated cell sorting (FACS), we identified a Sca-1+/ CD34+/ lin- (PECAM-: CD45-: Ter119-) population in the adult murine bladder. These cells were localized to the stromal layer of the adult bladder and appeared by postnatal day 1. Cultured Sca-1+/ CD34+/ lin- bladder cells self-renewed, formed colonies and spontaneously differentiated into cells expressing smooth muscle genes. These cells differentiated into other mesenchymal lineages (chondrocytes, adipocytes and osteocytes) upon culture in induction medium. Both acute and partial obstruction of the bladder reduced expression of CD34 and changed localization of Sca-1 to the urothelium. Partial obstruction resulted in upregulation of fibrosis genes within the Sca-1+/CD34+/lin- population. Our data indicate a resident, mesenchymal stem cell population in the bladder that is altered by bladder obstruction. These findings provide new information about the cellular changes in the bladder that may be associated with bladder fibrosis. 相似文献
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Jessie Lilly Michael J. Dadswell Montana F. McLean Trevor S. Avery Perry D. Comolli Michael J. W. Stokesbury 《Zeitschrift fur angewandte Ichthyologie》2021,37(6):826-834
In 2009, the Fundy Ocean Research Centre for Energy (FORCE) developed a 1.6 x 1.0 km Crown Lease Area (CLA) to test Marine Hydrokinetic devices (MHKs) in Minas Passage (MP), a strait that connects the Bay of Fundy to Minas Basin (MB), Nova Scotia. Minas Basin is an important summer feeding aggregation site for numerous fishes including Atlantic sturgeon (Acipenser oxyrinchus Mitchill, 1815) stocks from Canada and the US. In this study, acoustic tagging technology was used to describe sturgeon presence in the CLA, as well as an adjacent site proposed for MHKs deployment. During 2018 and 2019, 33.9% (n = 19/56 Atlantic sturgeon tagged) and 29.9% (n = 23/77 Atlantic sturgeon tagged) respectively, of sturgeon acoustically tagged in MB during 2014–2019 were detected in these two FORCE sites. Binomial General Linear Models (GLMs) were fit to determine if environmental parameters influenced the presence, or depth of Atlantic sturgeon in the FORCE sites. The only significant predictor of Atlantic sturgeon depth and presence at the FORCE sites was tide type. Atlantic sturgeon was found to have a higher likelihood of being detected during ebb tide (12%) as opposed to flood tide (3%). Apparent decline in the presence of sturgeon during flood tide was probably due to poor signal reception caused by turbulence from higher current speeds. Tagged sturgeon travelled through the FORCE sites pelagically at similar depths (27.5 ± 14.57 m) to those proposed for seabed mounted MHKs (~30 m), travelling deeper during flood tide than ebb, suggesting they may be at risk of spatial overlap with MHKs. While this study indicated that the duration Atlantic sturgeon utilized the FORCE sites throughout the summer appeared to be low, sturgeon from both endangered and threatened stocks migrated through this region during their yearly entry and exit to MB and were present for approximately 12% of the days assessed in this study. 相似文献
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Recovery from topkill of shortleaf pine × loblolly pine hybrids compared to their parent populations
Rodney E. Will Curtis J. Lilly John Stewart Scott Huff C. G. Tauer 《Trees - Structure and Function》2013,27(4):1167-1174
Hybrids between shortleaf pine (Pinus echinata Mill.) and loblolly pine (Pinus taeda L.) have increased since the 1950s throughout the southeastern USA. Previously, greater sprouting capacity and the formation of a basal crook that lowers the height of dormant buds may have favored pure shortleaf pine populations on fire prone sites. The objective of this study was to determine how seasonal timing of topkill by both fire and topclipping affect sprouting of shortleaf × loblolly pine F1 hybrids compared to their parent open-pollinated populations during their third growing season. A factorial combination of top-clipping (hand pruners) and girdling by fire (propane torch) was conducted on November 2010, January, March, and April 2011 and sprouting response was measured after the growing season. Survival of topkilled shortleaf pine (94 %) was greatest followed by hybrid (78 %) and loblolly pine (35 %). However, species effects varied with topkill treatment and treatment date because survival was relatively lower for loblolly and hybrid pines in the burn-only as well as the November and April treatment dates while survival of shortleaf pine was consistently high. The number of sprouts was greatest for shortleaf (32.3) intermediate for hybrid (23.8) and lowest for loblolly pine (12.0). Overall, 83 % of shortleaf pine, 35 % of hybrid pine, and 5 % of loblolly pine exhibited a basal crook. The height from ground line to the lowest sprout was shortest for shortleaf (3.5 mm), intermediate for hybrids (7.7 mm), and largest for loblolly pine (21.3 mm). While the hybrid saplings exhibited intermediate performance in survival, sprouting capacity, and crooking, pure shortleaf pine were superior and are probably better suited to recover from fire. 相似文献
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Tamama K Kawasaki H Kerpedjieva SS Guan J Ganju RK Sen CK 《Journal of cellular biochemistry》2011,112(3):804-817
Cell therapy with bone marrow multipotential stromal cells (MSCs) represents a promising approach to promote wound healing and tissue regeneration. MSCs expanded in vitro lose early progenitors with differentiation and therapeutic potentials under normoxic condition, whereas hypoxic condition promotes MSC self-renewal through preserving colony forming early progenitors and maintaining undifferentiated phenotypes. Hypoxia inducible factor (HIF) pathway is a crucial signaling pathway activated in hypoxic condition. We evaluated the roles of HIFs in MSC differentiation, colony formation, and paracrine activity under hypoxic condition. Hypoxic condition reversibly decreased osteogenic and adipogenic differentiation. Decrease of osteogenic differentiation depended on HIF pathway; whereas decrease of adipogenic differentiation depended on the activation of unfolded protein response (UPR), but not HIFs. Hypoxia-mediated increase of MSC colony formation was not HIF-dependent also. Hypoxic exposure increased secretion of VEGF, HGF, and basic FGF in a HIF-dependent manner. These findings suggest that HIF has a limited, but pivotal role in enhancing MSC self-renewal and growth factor secretions under hypoxic condition. 相似文献