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Mary Ann Lila Smith L. Art Spomer 《In vitro cellular & developmental biology. Plant》1987,23(1):67-70
Summary Objective, accurate, non-intrusive measurement of in vitro cell growth was realized through microcomputerized video image
analysis. Recently-released video and digitizing hardware and software were incorporated into an analytical system which accurately
quantified visual differences between cultures on a cell number or fresh mass basis. Sequential measurements during culture
incubation further detected and quantified subtle changes in colony area and density resulting from growth. Each measurement
was acquired rapidly, without encroaching on the in vitro environment, so cell growth was undisturbed. Custom software routines
coordinated the quantification of this detailed record into precise cumulative growth curves. 相似文献
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Sajad Hassanzadeh Maryam Jalessi Seyed Behnamedin Jameie Mehdi Khanmohammadi Zohre Bagher Zeinab Namjoo Seyed Mohammad Davachi 《Biochemistry and Biophysics Reports》2021
Functional improvement after spinal cord injury remains an unsolved difficulty. Glial scars, a major component of SCI lesions, are very effective in improving the rate of this recovery. Such scars are a result of complex interaction mechanisms involving three major cells, namely, astrocytes, oligodendrocytes, and microglia. In recent years, scientists have identified two subtypes of reactive astrocytes, namely, A1 astrocytes that induce the rapid death of neurons and oligodendrocytes, and A2 astrocytes that promote neuronal survival. Moreover, recent studies have suggested that the macrophage polarization state is more of a continuum between M1 and M2 macrophages. M1 macrophages that encourage the inflammation process kill their surrounding cells and inhibit cellular proliferation. In contrast, M2 macrophages promote cell proliferation, tissue growth, and regeneration. Furthermore, the ability of oligodendrocyte precursor cells to differentiate into adult oligodendrocytes or even neurons has been reviewed. Here, we first scrutinize recent findings on glial cell subtypes and their beneficial or detrimental effects after spinal cord injury. Second, we discuss how we may be able to help the functional recovery process after injury. 相似文献