全文获取类型
收费全文 | 837篇 |
免费 | 67篇 |
出版年
2022年 | 6篇 |
2021年 | 20篇 |
2020年 | 11篇 |
2019年 | 9篇 |
2018年 | 18篇 |
2017年 | 21篇 |
2016年 | 17篇 |
2015年 | 34篇 |
2014年 | 27篇 |
2013年 | 55篇 |
2012年 | 62篇 |
2011年 | 58篇 |
2010年 | 44篇 |
2009年 | 27篇 |
2008年 | 30篇 |
2007年 | 36篇 |
2006年 | 38篇 |
2005年 | 40篇 |
2004年 | 30篇 |
2003年 | 33篇 |
2002年 | 27篇 |
2001年 | 17篇 |
2000年 | 9篇 |
1999年 | 18篇 |
1998年 | 9篇 |
1997年 | 7篇 |
1996年 | 6篇 |
1995年 | 7篇 |
1994年 | 5篇 |
1993年 | 9篇 |
1992年 | 12篇 |
1991年 | 12篇 |
1990年 | 13篇 |
1989年 | 14篇 |
1988年 | 4篇 |
1986年 | 5篇 |
1985年 | 7篇 |
1984年 | 7篇 |
1981年 | 6篇 |
1979年 | 8篇 |
1978年 | 4篇 |
1977年 | 6篇 |
1976年 | 5篇 |
1975年 | 4篇 |
1972年 | 3篇 |
1962年 | 4篇 |
1961年 | 3篇 |
1957年 | 3篇 |
1950年 | 3篇 |
1934年 | 4篇 |
排序方式: 共有904条查询结果,搜索用时 771 毫秒
191.
In DNA of the dinoflagellate Crypthecodinium cohnii, 38% of the thymine is replaced by the modified base 5-hydroxymethyluracil, and approximately 3% of the cytosine is replaced by 5-methylcytosine. Both of the modified bases are non-randomly distributed in the DNA. Determinations of 3' nearest neighbors show that HOMeU is preferentially located in the dinucleotides HOMeUpA and HOMeUpC. Pyrimidine tract analysis shows that HOMeU is also greatly enriched in the trinucleotide purine-HOMeU-purine. As in other eukaryotes, methylcytosine in C. cohnii DNA occurs predominantly in the dinucleotide MeCpG. By analysis of restriction endonuclease digestion patterns of C. cohnii total DNA and ribosomal DNA, we have found that the central CpG dinucleotides in the sites for the enzymes Hpa II (CCGG) and Hha I (GCGC) are extensively methylated in both total DNA and ribosomal DNA. Results of digestion with Ava I, however, indicated that not all CpG dinucleotides in the sequence CCTCGGAG are methylated in C. cohnii DNA. 相似文献
192.
193.
Production of exotoxins by Aeromonas spp. at 5°C 总被引:2,自引:0,他引:2
The ability of 60 strains of Aeromonas to produce enterotoxin and haemolysin after cultivation at 5°C for 7–10 d was investigated. The strains were isolated from lamb meat, offal, carcasses and faeces, and had previously been tested for their ability to produce these exotoxins at 37°C. The results showed that some strains of Aeromonas hydrophila and A. sobria were capable of producing enterotoxin and haemolysin at 5°C, but none of the A. caviae strains tested produced these two factors. Of the 30 A. hydrophila strains investigated 25 and 27 were enterotoxigenic and haemolytic respectively. Likewise, of the 24 A. sobria strains investigated 16 and 18 were enterotoxigenic and haemolytic respectively. The results indicate that certain strains of Aeromonas species, in particular A. hydrophila and A. sobria , are of potential public health significance in meats stored at refrigeration temperature. 相似文献
194.
T C Rae 《Folia primatologica; international journal of primatology》1999,70(3):125-135
The initial appearance of hominoids, or apes, and the selective pressures that led to their emergence are currently disputed. Central to the argument are the proconsulids, variously described as the earliest apes or as stem catarrhines, based on facial and postcranial data, respectively. The present paper reports on incongruence and parsimony analyses applied to a combined data set. The results demonstrate that proconsulids are cladistic hominoids, and that the apparent incongruence between the data sets is due to mosaic evolution; the earliest changes in Hominoidea occurred in the face. These results suggest that the initial divergence of hominoids involved selection for an ape-like face, and was not driven by an adaptive shift to below-branch locomotion. 相似文献
195.
196.
P J Schmidt T D Rae R A Pufahl T Hamma J Strain T V O'Halloran V C Culotta 《The Journal of biological chemistry》1999,274(34):23719-23725
The copper chaperone for superoxide dismutase (SOD1) inserts the catalytic metal cofactor into SOD1 by an unknown mechanism. We demonstrate here that this process involves the cooperation of three distinct regions of the copper chaperone for SOD1 (CCS): an amino-terminal Domain I homologous to the Atx1p metallochaperone, a central portion (Domain II) homologous to SOD1, and a short carboxyl-terminal peptide unique to CCS molecules (Domain III). These regions fold into distinct polypeptide domains as revealed through proteolysis protection studies. The biological roles of the yeast CCS domains were examined in yeast cells. Surprisingly, Domain I was found to be necessary only under conditions of strict copper limitation. Domain I and Atx1p were not interchangeable in vivo, underscoring the specificity of the corresponding metallochaperones. A putative copper site in Domain II was found to be irrelevant to yeast CCS activity, but SOD1 activation invariably required a CXC in Domain III that binds copper. Copper binding to purified yeast CCS induced allosteric conformational changes in Domain III and also enhanced homodimer formation of the polypeptide. Our results are consistent with a model whereby Domain I recruits cellular copper, Domain II facilitates target recognition, and Domain III, perhaps in concert with Domain I, mediates copper insertion into apo-SOD1. 相似文献
197.
198.
Oxygen-limited cultures of Klebsiella pneumoniae reduced 4-methylsulfinyl phenol to 4-methylthiophenol. A study of the effect of 4-methylthiophenol on the growth of K. pneumoniae revealed that the specific growth rate was retarded by 40% in the presence of 200 micrograms of the phenol per ml. A soil bacterium, Hafnia sp., was isolated that could reduce the organophosphorus insecticide fensulfothion to fensulfothion sulfide. 相似文献
199.
200.
Structures in a blue-green alga resembling prolamellar bodies 总被引:3,自引:0,他引:3
Summary Cells ofAnabaena (IUCC 380) from soil-water cultures 106 and 168 days after inoculation were fixed in 2% KMnO4 and studied by electron microscopy. Thylakoid lattices morphologically similar to prolamellar bodies characteristic of etiolated higher plant chloroplasts were discovered. Prefixation sonication and centrifugation were eliminated as factors producing these lattices in the photosynthetic thylakoid system of these prokaryotic cells. Similar lattices are not seen in young cultures in the log phase of growth cultured under the same temperature and light conditions in the same medium. Factors, other than age, producing these lattices are yet to be determined. 相似文献