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101.
Four c-type cytochromes were purified by several procedures including chromatography on DEAE-Sepharose CL-6B, Phenyl-Sepharose CL-4B and Sephadex G-75, G-100 and G-200 and chromatofocusing. Cytochrome c-551 had a pI value of 5.2 and an Mr of 260 000 consisting of six non-covalently bound polypeptides each with an Mr of 43 000, and contained four to five haems. Cytochrome c-552.5 had a pI value of 4.8 and an Mr of 56 000 consisting of two polypeptides with the same Mr 29 000, and contained two haems. Cytochromes c-551 and c-552.5 were reduced by ascorbate to about 70 and 60% of the fully dithionite-reduced values, respectively, and both were essential components in the thiosulphate-oxidizing multi-enzyme system (other components of the system were ‘enzyme A’, ‘enzyme B’ and sulphite: cytochrome c oxidoreductase). These two cytochromes functioned as electron carriers and effectors in the oxidation of thiosulphate. Some evidence suggested that cytochrome c-551 might be a specialized electron transfer component for sulphonate-sulphur oxidation. Both cytochromes could be reduced by thiosulphate in the presence of enzymes A and B. Cytochrome c-550 (basic) and cytochrome c-550 (acidic) were small proteins with Mr 15 000 and 14 000 and pI values of over 8 and 5, respectively. Their physiological role is uncertain. 相似文献
102.
103.
Dimethyl sulfoxide present in the agar medium at concentration 0.2 % (v/v) and lower does not inhibit cytokinin-induced betacyanin
synthesis in theAmaranthus caudatus seedlings. The activity of kinetin, N6-(Δ2-isopentenyl)adenine andtrans- zeatin is the same when these cytokinins are dissolved in either water or dimethyl sulfoxide and incorporated into the medium
after autoclaving. A simple method is described which allows the cytokinin activity of slightly water-soluble and thermolabile
compounds,e.g. aromatic urea and thiourea derivatives, to be determined in theAmaranthus bioassay. 相似文献
104.
G C DuBois E Appella R Armstrong W Levin A Y Lu D M Jerina 《The Journal of biological chemistry》1979,254(14):6240-6243
Highly purified hepatic microsomal epoxide hydrase, which had been purified in the presence of proteolytic enzyme inhibitors, was subjected to carboxypeptidase Y digestion, automated Edman degradation, and carbohydrate analysis. Carboxypeptidase Y digestion resulted in the near stoichiometric release of leucine, the COOH-terminal amino acid. Automated Edman degradation permitted the identification of the first 20 amino acid residues of epoxide hydrase. Methionine was identified as the NH2-terminal residue. The NH2-terminal region of epoxide hydrase is similar in hydrophobicity to the NH2-terminal precursor segments of several secretory proteins and the NH2-terminal regions of several microsomal cytochromes P-450. Carbohydrate analyses of the enzyme revealed the presence of 0.5 to 1.0 mol of mannose/50,000 g of protein. These results provide evidence for the presence of a single polypeptide chain in our purified enzyme preparations and suggest that there may be only one enzymic form of epoxide hydrase in microsomes from phenobarbital-treated rats. 相似文献
105.
Possible molecular detent in the DNA structure at regulatory sequences 总被引:10,自引:0,他引:10
A common feature that appears in a number of DNA sites where proteins interact is the sequence GTG/CAC. In the lac operator this sequence leads to a region with a higher imino proton exchange rate well below the optical melting temperature. It is suggested that this reflects a structural feature recognized by proteins that bind specific sites on the DNA molecule. 相似文献
106.
Purified liver microsomal NADPH-cytochrome P-450 reductase is able to catalyze the activation of [14C]ronidazole to metabolite(s) which bind covalently to protein. Like the reaction catalyzed by microsomes, protein alkylation catalyzed by the reductase is (1) sensitive to oxygen, (2) requires reducing equivalents, (3) is inhibited by sulfhydryl-containing compounds and (4) is stimulated several fold by either flavin mononucleotide (FMN) or methytlviologen. A cytochrome P-450 dependent pathway of ronidazole activation can be demonstrated as judged by the inhibition of the reaction by carbon monoxide, metyrapone and 2,4-dichloro-6-phenylphenoxyethylamine but the involvement of specific microsomal cytochrome P-450 isozymes has not been definitively established. Milk xanthine oxidase is also capable of catalyzing ronidazole activation. Polyacrylamide sodium dodecyl sulfate (SDS)-gel electrophoresis reveals that the reactive intermediate(s) of ronidazole does not alkylate proteins selectively. 相似文献
107.
Ronidazole (1-methyl-5-nitroimidazole-2-methanol carbamate) is reductively metabolized by liver microsomal and purified NADPH-cytochrome P-450 reductase preparations to reactive metabolites that covalently bind to tissue proteins. Kinetic experiments and studies employing immobilized cysteine or blocked cysteine thiols have shown that the principal targets of protein alkylation ara cysteine thiols. Furthermore, ronidazole specifically radiolabelled with 14C in the 4,5-ring, N-methyl or 2-methylene positions give rise to equivalent apparent covalent binding suggesting that the imidazole nucleus is retained in the bound residue. In contrast, the carbonyl-14C-labeled ronidazole gives approx. 6--15-fold less apparent covalent binding indicating that the carbamoyl group is lost during the reaction leading to the covalently bound metabolite. The conversion of ronidazole to reactive metabolite(s) is quantitative and reflects the amazing efficiency by which this compound is activated by microsomal enzymes. However, only about 5% of this metabolite can be accounted for as protein-bound products under the conditions employed in these studies. Consequently, approx. 95% of the reactive ronidazole metabolite(s) can react with other constituents in the reaction media such as other thiols or water. Based on these results, a mechanism is proposed for the metabolic activation of ronidazole. 相似文献
108.
去传入神经可引起后角一些肽类变化和含肽的大颗粒小泡以胞吐的形式在非突触部位释放。为了探讨这一作用机制,本文切除一侧刚髭部皮肤,应用免疫组化方法观察并定量分析术后不同时间延髓后角浅层SP、CCK、NT、L-ENK阳性纤维的变化及利多卡因对后角浅层SP的影响。结果显示:术后1小时,延髓后角浅层SP损伤侧较对照侧升高,第3~7天未见显著性减少或升高,第8天开始脱失(P<0.05),第14天脱失更甚,第30天开始恢复,第90~120天基本恢复。CCK变化基本同SP。NT、L-ENK术后不同时间(7~120天),两侧比较未见显著性差异(P>0.05)。应用和多卡因后,后角浅层SP损伤侧较对照侧未见明显减少(P>0.05)。根据上述结果,本文推测:去传入神经导致延髓后角浅层神经肽的变化,可能是神经损伤区(神经瘤或再生芽)产生异常电活动,引起延髓后角浅层神经肽释放的结果。 相似文献
109.
应用组织培养方法选育耐盐碱水稻新品种 总被引:2,自引:0,他引:2
本文报道了应用组织培养方法,选育耐盐碱水稻.作法是将去壳的水稻种子经常规消毒接种在脱分化培养基诱导形成愈伤组织,继代一次,转移到加压培养基(MS+2.4-D2mg/L+NaCl1%+NaHCO_30.5%),处理20天,再转移到分化培养基,分化并培育出组培水稻119份,简称为D水稻.D水稻又经过盐碱地的实际种植,大批D水稻被田间粗筛而淘汰掉,保存下20份从形态表现和耐盐碱性较好的材料.然后对20份D水稻的脯氨酸含量和叶绿素含量进行测定分析并与其耐盐碱性进行比较,把各项指标综合起来鉴定其耐盐碱性,最终筛选出7价为耐盐碱性较强的水稻新品系.其中647—4表现为最耐盐碱、抗病、高产的水稻. 相似文献
110.
本文通过多年多点的田间接种试验,分析了大豆根瘤菌C_(33)(系VSDA_(110)的突变株)的增产效应.在合丰25号大豆接种C_(33)取得明显的增产效果,两年平均比CK增产28.55%,比61A76增产24.5%;C_(33)接种在其他品种以及在不同类型和肥力的土壤上增产效果也均高于CK和61A76,说明大豆根瘤菌C_(33)比当前生产上应用的61A76更具有广谱性和高效性. 相似文献