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61.
Synthetic corticosteroids are widely used as anti-inflammatory agents. Mechanisms of their degradation continue to be studied. D-ring homoannulation is a well-known metabolic pathway for steroids in vivo. The rearrangement with aluminium trichloride of the commercial anti-inflammatory drugs hydrocortisone, cortisone and dexamethasone is here presented. The structures of the corresponding 17a-keto-17-hydroxy-D-homosteroids are established by mono- and two-dimensional NMR analysis. Inversion of the alpha-configuration of C-16 is observed in the Lewis acid assisted D-homoannulation of dexamethasone. 相似文献
62.
Abstract— The DNA polymerase of the optic lobes of Octopus vulgaris Lam has been solubilized and some of its properties have been investigated. The enzyme catalyses the incorporation of [3 H]thymidine triphosphate into a DNase-sensitive, acid-insoluble product in the presence of DNA, the four deoxynucleoside triphosphates and Mg2+ . The pH optimum is between pH 9.2 and 9.6, the optimal concentration of Mg2+ is between 10 and 30 mM and the optimal temperature is in the range 27°-30°C. The content of enzyme per mg protein is highest in the vertical and optic lobes and lowest in the suboesophageal lobe. 相似文献
63.
Cimmaruta Chiara Citro Valentina Andreotti Giuseppina Liguori Ludovica Cubellis Maria Vittoria Hay Mele Bruno 《BMC bioinformatics》2018,19(15):433-46
Background
Severity gradation of missense mutations is a big challenge for exome annotation. Predictors of deleteriousness that are most frequently used to filter variants found by next generation sequencing, produce qualitative predictions, but also numerical scores. It has never been tested if these scores correlate with disease severity.Results
wANNOVAR, a popular tool that can generate several different types of deleteriousness-prediction scores, was tested on Fabry disease. This pathology, which is caused by a deficit of lysosomal alpha-galactosidase, has a very large genotypic and phenotypic spectrum and offers the possibility of associating a quantitative measure of the damage caused by mutations to the functioning of the enzyme in the cells. Some predictors, and in particular VEST3 and PolyPhen2 provide scores that correlate with the severity of lysosomal alpha-galactosidase mutations in a statistically significant way.Conclusions
Sorting disease mutations by severity is possible and offers advantages over binary classification. Dataset for testing and training in silico predictors can be obtained by transient transfection and evaluation of residual activity of mutants in cell extracts. This approach consents to quantitative data for severe, mild and non pathological variants.64.
Angelo Liguori Giovanni Sindona Nicola Uccella 《Nucleosides, nucleotides & nucleic acids》2013,32(3):373-377
Abstract The effect of benzoyl substitution on the unimolecular depurination of deoxyadenosine, in the gas-phase, has been evaluated. The glycosidic bond dissociation of the conjugated acids and bases of the isomeric species occurs remote fran the charge sites. 相似文献
65.
B. Wirth D. Tessarolo E. Hahnen S. Rudnik-Schöneborn H. Raschke M. Liguori M. Giacanelli K. Zerres 《Human genetics》1997,100(5-6):676-680
The molecular analysis of the survival motor neuron (SMN) gene and several closely flanking polymorphic markers in an atypical
pedigree with four patients suffering from spinal muscular atrophy (SMA) over two generations has raised new aspects concerning
the etiology and the molecular spectrum of autosomal recessive SMA. Three patients in two generations show homozygous deletions
of exons 7 and 8 of the telomeric copy of SMN (telSMN), thus confirming the presence of autosomal recessive SMA, with localisation
on chromosome 5q12. The fourth SMA patient with mild neurogenic atrophy (confirmed by muscle biopsy and electromyography)
shows no homozygous deletion of telSMN but carries a heterozygous deletion of telSMN, as can be deduced from her two affected
homozygously deleted children. No intragenic mutation has been identified in the remaining telSMN. In addition, she shares
only one SMA chromosome with her affected brother, is haploidentical with two healthy brothers, and has a 31-year-old healthy
son, who has inherited an SMN-deleted paternal chromosome and the SMN non-deleted maternal chromosome. These results suggest
that this patient either has a neurogenic atrophy of a different origin or exhibits an unusual heterozygous manifestation
of SMA 5q12. Interestingly, the two haploidentical telSMN-deleted affected sibs in the second generation show a strikingly
discordant clinical picture indicating that, in addition to telSMN mutations, other factors influence the phenotype of SMA
in the reported pedigree.
Received: 20 March 1997 / Accepted: 4 June 1997 相似文献
66.
The effect of ACTH and electric foot shock stress on DOPAC content were determined in the frontal cortex and nucleus accumbens. Twenty min of stress enhanced DOPAC levels in the frontal cortex and in the nucleus accumbens by about 80% and 35%, respectively. On the other hand, a single dose of ACTH failed to change DOPAC concentration in the above brain areas. The present results show that the activation of mesolimbic and mesocortical dopaminergic systems is not mediated by ACTH secretion. 相似文献
67.
68.
Pasqualino De Antonellis Marianeve Carotenuto Jonathan Vandenbussche Gennaro De Vita Veronica Ferrucci Chiara Medaglia Iolanda Boffa Alessandra Galiero Sarah Di Somma Daniela Magliulo Nadia Aiese Alessandro Alonzi Daniela Spano Lucia Liguori Cristina Chiarolla Antonio Verrico Johannes H. Schulte Pieter Mestdagh Jo Vandesompele Kris Gevaert Massimo Zollo 《Molecular & cellular proteomics : MCP》2014,13(8):2114-2131
69.
Tajvur P Saber CT Ng Guillaume Renard Bernadette M Lynch Eliza Pontifex Ceara AE Walsh Alexia Grier Marian Molloy Barry Bresnihan Oliver FitzGerald Ursula Fearon Douglas J Veale 《Arthritis research & therapy》2010,12(3):1-6
Introduction
Since remission is now possible in psoriatic arthritis (PsA) we wished to examine remission rates in PsA patients following anti tumour necrosis factor alpha (TNFα) therapy and to examine possible predictors of response.Methods
Analysis of a prospective patient cohort attending a biologic clinic, between November 2004 and March 2008, was performed prior to commencing therapy and at regular intervals. Baseline clinical characteristics including demographics, previous disease-modifying antirheumatic drug (DMARD) response, tender and swollen joint counts, early morning stiffness, pain visual analogue score, patient global assessment, C reactive protein (CRP) and health assessment questionnaire (HAQ) were collected.Results
A total of 473 patients (152 PsA; 321 rheumatoid arthritis (RA)) were analyzed. At 12 months remission, defined according to the disease activity score using 28 joint count and CRP (DAS28-CRP), was achieved in 58% of PsA patients compared to 44% of RA patients, significant improvement in outcome measures were noted in both groups (P < 0.05). Analysis of a subgroup of PsA and RA patients matched for DAS28-CRP at baseline also showed higher numbers of PsA patients achieving remission. Linear regression analysis identified the HAQ at baseline as the best predictor of remission in PsA patients (P < 0.001).Conclusions
DAS28 remission is possible in PsA patients at one year following anti-TNF therapy, at higher rates than in RA patients and is predicted by baseline HAQ. 相似文献70.