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Lavinia Liguori Barry Stidder Jean-Pierre Alcaraz Jean-Luc Lenormand Philippe Cinquin Donald K. Martin 《Preparative biochemistry & biotechnology》2016,46(6):546-551
The mitochondrial voltage-dependent anion channel (VDAC) is a pivotal protein since it provides the major transport pathway between the cytosol and the mitochondrial intermembrane space and it is implicated in cell apoptosis by functioning as a gatekeeper for the trafficking of mitochondrial death molecules. VDAC is a beta-barrel channel with a large conductance, and we use it as a model transport protein for the design of biomimetic systems. To overcome the limitations of classical overexpression methods for producing and purifying membrane proteins (MPs) we describe here the use of an optimized cell-free system. In a one-step reaction VDAC is obtained directly integrated into liposomes and purified by ultracentrifugation. We then combine proteoliposomes with different bilayers models in order to validate VDAC insertion and functionality. This VDAC biomimetic model is the first example validating the use of a cell-free expression system for production of MPs into liposomes and tethered bilayers as a toolbox to build a wide range of biomimetic devices. 相似文献
163.
Montano Simone Maggioni Davide Liguori Giulia Arrigoni Roberto Berumen Michael L. Seveso Davide Galli Paolo Hoeksema Bert W. 《Coral reefs (Online)》2020,39(2):375-386
Coral Reefs - Coral diseases are emerging as a major threat to coral reefs worldwide, and although many of them have been described, knowledge on their epizootiology is still limited. This is the... 相似文献
164.
In order to compare the disposition of D-galactose and D-glucose in various organs of the rat, we have measured the amount of 14C-galactose and 14C-glucose present in the enteric canal, blood, muscle and liver, 2h. after the oral administration of the labelled esoses. Our results show that the two esoses are ebsorbed almost completely and to the same extent by two hours after their administration, Galactose has a longer half-life than glucose, being more slowly utilized in the synthesis of glycogen in liver and muscle. 相似文献
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S Senatore G S Jotti D Liguori M Chetrì A Attolini G Cristofaro M Gabrielli 《The International journal of biological markers》1986,1(3):137-140
Gastrointestinal biopsies from 18 members of a family with Lynch Syndrome II were evaluated and immunocytochemical studies were made to characterize the phenotypic expression of the tissue's immune populations. The intestinal findings suggest polyclonal B-cell activation related to the T-helper distribution. Our evaluation provides no specific information so far on the management of patients with Lynch Syndrome II. 相似文献
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Inactivation of tyrosine aminotransferase in neutral homogenates and rat liver slices 总被引:4,自引:4,他引:0 下载免费PDF全文
Inactivation of tyrosine aminotransferase induced in vivo by triamcinolone was studied in a homogenate incubated at neutral pH values. The integrity and the presence of subcellular particles together with a compartment of acidic pH are necessary for inactivation of tyrosine aminotransferase. It is suggested that tyrosine aminotransferase is inactivated inside lysosomes. The system responsible for inactivation of tyrosine aminotransferase was partially purified and identified with lysosomal cathepsins B and B(1). Inactivation of tyrosine aminotransferase in liver slices is controlled by the amino acid concentration and strongly stimulated by cysteine. 3,3',5-Tri-iodo-l-thyronine reversibly and strongly decreases the rate of inactivation of tyrosine aminotransferase. The effect is not due to an increased rate of tyrosine aminotransferase synthesis. 相似文献
169.