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141.
Autologous disc cell implantation, growth factors and gene therapy appear to be promising therapies for disc regeneration. Unfortunately, the replicative lifespan and growth kinetics of human nucleus pulposus (NP) cells related to host age are unclear. We investigated the potential relations among age, replicative lifespan and growth rate of NP cells, and determined the age range that is suitable for cell-based biological therapies for degenerative disc diseases. We used NP tissues classified by decade into five age groups: 30s, 40s, 50s, 60s and 70s. The mean cumulative population doubling level (PDL) and population doubling rate (PDR) of NP cells were assessed by decade. We also investigated correlations between cumulative PDL and age, and between PDR and age. The mean cumulative PDL and PDR decreased significantly in patients in their 60s. The mean cumulative PDL and PDR in the younger groups (30s, 40s and 50s) were significantly higher than those in the older groups (60s and 70s). There also were significant negative correlations between cumulative PDL and age, and between PDR and age. We found that the replicative lifespan and growth rate of human NP cells decreased with age. The replicative potential of NP cells decreased significantly in patients 60 years old and older. Young individuals less than 60 years old may be suitable candidates for NP cell-based biological therapies for treating degenerative disc diseases. 相似文献
142.
Jayaraman Selvaraj Shazia Fathima JH Venkatacalam Sivabalan Umapathy Vidhya Rekha Rajagopal Ponnulakshmi Veeraraghavan Vishnupriya Malathi Kullappan Radhika Nalinakumari Sreekandan Surapaneni Krishna Mohan Periyasamy Vijayalakshmi 《Bioinformation》2021,17(1):200
Cornulin (CRNN) is linked with tumour progression. Therefore, it is of interest to document data on the molecular modeling of cornulin (CRNN) for docking with phytocompounds (Pyrazinamide, Anisotine, Vasicinone, Vasicoline) from Justicia adhatoda L. Thus, we document the optimal binding features of these compounds with the cornulin model for further consideration. 相似文献
143.
Jayaraman Selvaraj Umapathy Vidhya Rekha Shazia Fathima JH Venkatacalam Sivabalan Rajagopal Ponnulakshmi Veeraraghavan Vishnupriya Malathi Kullappan Radhika nalinakumari Sreekandan Surapaneni Krishna Mohan 《Bioinformation》2021,17(1):167
It is of interest to document the moelcular docking analysis of SARS-CoV-2 linked RNA dependent RNA polymerase (RdRp) with compounds from Plectranthus amboinicus. Hence, we report the binding features of rutin, Luteolin, Salvianolic acid A, Rosmarinic acid and p-Coumaric acid with the target protein SARS-CoV-2 linked RNA dependent RNA polymerase (RdRp) for further consideration. 相似文献
144.
Hans-Christian Siebert Shan-Yun Lu Rainer Wechselberger Karin Born Thomas Eckert Songping Liang Claus-Wilhelm von der Lieth Jesús Jimnez-Barbero Roland Schauer Johannes F.G. Vliegenthart Thomas Lütteke Tibor Kor 《Carbohydrate research》2009,344(12):1515-16285
The affinity to sialic acid-containing oligosaccharides of the small-animal lectin SHL-I isolated from the venom of the Chinese bird-hunting spider Selenocosmia huwena is here described for the first time. By a strategic combination of NMR techniques, molecular modeling, and data mining tools it was possible to identify the crucial amino acid residues that are responsible for SHL-I’s ability to bind sialic acid residues in a specific way. Furthermore, we are able to discuss the role of the functional groups of sialic acid when bound to SHL-I. Also the impact of Pro31 in its cis- or trans-form on SHL-I’s ligand affinity is of special interest, since it answers the question if Trp32 is a crucial amino acid for stabilizing complexes between SHL-I and sialic acid. SHL-I can be considered as a proper model system that provides further insights into the binding mechanisms of small-animal lectins to sialic acid on a sub-molecular level. 相似文献
145.
At the Human Disease Glycomics/Proteome Initiative workshop held on August 27 on the occasion of the fourth HUPO Congress in Munich, Germany, the working groups reported on pilot studies, performed by 21 laboratories world-wide, to analyze glycan using MS. During the steering committee on July 31 in Osaka, Japan, it was reported that these groups have developed a system using MS to assess the world-wide network on the screening of congenital disorders of glycosylation. The concept of glycobioinformatics was also explained at this meeting. The session on PTMs laid particular emphasis on the importance of functional glycomics using glycosyltransferase genes as well as on the importance of identifying the target protein(s) carrying the sugar chains. 相似文献
146.
Harald Lahm Sabine André Andreas Hoeflich Herbert Kaltner Hans-Christian Siebert Bernard Sordat Claus-Wilhelm von der Lieth Eckhard Wolf Hans-Joachim Gabius 《Glycoconjugate journal》2003,20(4):227-238
β-Galactosides of cell surface glycoconjugates are docking sites for endogenous lectins of the galectin family. In cancer
cells, primarily galectins-1 and -3 have been studied to date. With the emergence of insights into their role in growth control,
resistance to or induction of apoptosis and invasive behavior the notion is supported that they can be considered as functional
tumor markers. In principle, the same might hold true for the other members of the galectin family. But their expression in
tumors has hitherto been a subject of attention only to a very limited extent. Pursuing our concept to define the complexity
of the galectin network in cancer cells and the degree of functional overlap/divergence with diagnostic/therapeutic implications,
we have introduced comprehensive RT-PCR monitoring to map their galectin gene expression. The data on so far less appreciated
galectins in this context such as galectins-4 and -8 vindicate this approach. They, too, attach value to extend the immunohistochemical
panel accordingly. Our initial histopathological and cell biological studies, for example on colon cancer progression, prove
the merit of this procedure. Aside from the detection of gene expression profiles by RT-PCR, the detailed molecular biological
monitoring yielded further important information. We describe different levels of regulation of galectin production in colon
cancer cells in the cases of the tandem-repeat-type galectins-8 and -9. Isoforms for them are present with insertions into
the peptide linker sequence attributed to alternative splicing. Furthermore, variants with distinct amino acid substitutions
(galectin-8, Po66-CBP, PCTA-1, CocaI/II and galectin-9/ecalectin) and generation of multiple mRNA species, notably those coding
for truncated galectin-8 and -9 versions with only one lectin site, justify to portray these two family members not as distinct
individuals but as groups. In aggregate, the ongoing work to thoroughly chart the galectin network and to disentangle the
individual functional contributions is expected to make its mark on our understanding of the malignant phenotype in certain
tumor types. Published in 2004.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
147.
Emadeddin Tajkhorshid Hans-Christian Siebert Maria Burchert Herbert Kaltner Gian Kayser Claus-Wilhelm von der Lieth Robert Kaptein Johannes F. G. Vliegenthart Hans-Joachim Gabius 《Journal of molecular modeling》1997,3(8):325-331
Galectins (Galactose binding lectins) from bacteria, plants and animals have been shown to possess tyrosine or tryptophan residues that form hydrophobic contacts with their ligands in the binding sites. At the present time, the X-ray structures of only two galectins from human and bovine tissues are known. In the present study we applied X-ray data of bovine heart galectin-1 as a template for homology modelling of a number of galectins from mammalian and avian tissues. The conservation of one tryptophan and at least one histidine in binding pocket can be observed from the comparison of the model structures. We also show that it is possible to obtain information of the architecture of the binding pocket of several galectins in solution using CIDNP (Chemically Induced Dynamic Nuclear Polarisation) techniques. The CIDNP approach offers a possibility to analyse these lectins in solution thereby providing supplementary information to the available X-ray data. All studied galectins show comparable alterations when they are recorded by CIDNP-technique in the absence and in the presence of their specific carbohydrate ligands. 相似文献
148.
Umapathy Vidhya Rekha Ponnulakshmi Rajagopal Rama Varadhachariyar Puja Harie Priya JH Shazia Fathima Ramajayam Govindan ChellaPerumal Palanisamy KB Aswini Brindha Vishnu Priya Veeraraghavan Selvaraj Jayaraman 《Bioinformation》2022,18(3):265
We document the Molecular docking analysis of bioactive compounds from Cissampelos pareira with PPAR gamma for further consideration in drug discovery for T2DM. 相似文献
149.
Dr. Helmut Lieth 《Planta》1960,54(6):555-576
Ohne ZusammenfassungMit 9 Textabbildungen 相似文献
150.