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EB Adamah-Biassi Y Zhang H Jung S Vissapragada RJ Miller ML Dubocovich 《The journal of histochemistry and cytochemistry》2014,62(1):70-84
The pineal hormone melatonin activates two G-protein coupled receptors (MT1 and MT2) to regulate in part biological functions. The MT1 and MT2 melatonin receptors are heterogeneously distributed in the mammalian brain including humans. In the mouse, only a few reports have assessed the expression of the MT1 melatonin receptor expression using 2-iodomelatonin binding, in situ hybridization and/or polymerase chain reaction (PCR). Here, we described a transgenic mouse in which red fluorescence protein (RFP) is expressed under the control of the endogenous MT1 promoter, by inserting RFP cDNA at the start codon of MTNR1a gene within a bacterial artificial chromosome (BAC) and expressing this construct as a transgene. The expression of RFP in the brain of this mouse was examined either directly under a fluorescent microscope or immunohistochemically using an antibody against RFP (RFP-MT1). RFP-MT1 expression was observed in many brain regions including the subcommissural organ, parts of the ependyma lining the lateral and third ventricles, the aqueduct, the hippocampus, the cerebellum, the pars tuberalis, the habenula and the habenula commissure. This RFP-MT1 transgenic model provides a unique tool for studying the distribution of the MT1 receptor in the brain of mice, its cell-specific expression and its function in vivo. 相似文献
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Pregnancy block in mice requires exposure of recently mated females to
urinary pheromones of a strange male, and when working with inbred strains
this invariably requires urine from an outbred line. The pheromones which
induce oestrus and early puberty in mice have been identified as the
brevicomins and dihydrothiazoles. Since the same vomeronasal, neural and
neuroendocrine pathways are also activated in pregnancy block, these
compounds are likely candidates for pregnancy blocking pheromones. However,
these relatively simple chemicals lack the capacity to code for differing
mouse strains. Since large quantities of the polymorphic major urinary
proteins from the lipocalin family found in urine serve as transporters for
the dihydrothiazoles and brevicomins, and differ across strains, then these
proteins must participate in pheromone recognition in the context of
pregnancy block.
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