Hereditary bleaching ofEuglena gracilis affected by 3-(5-nitro-2-furyl)acrylic acid

Light and elevated incubation temperature were found to be involved in the ability of four nitrofurans to cause the hereditary bleaching effect inEuglena gracilis. The enzyme activation by S9 mix also played a significant role in the case of 3-(5-nitro-2-furyl)acrylic acid. When using the combination of the above factors the most pronounced hereditary bleaching was reached.     

A threshold of mechanical strain intensity for the direct activation of osteoblast function exists in a murine maxilla loading model

The response to the mechanical loading of bone tissue has been extensively investigated; however, precisely how much strain intensity is necessary to promote bone formation remains unclear. Combination studies utilizing histomorphometric and numerical analyses were performed using the established murine maxilla loading model to clarify the threshold of mechanical strain needed to accelerate bone formation activity. For 7 days, 191 kPa loading stimulation for 30 min/day was applied to C57BL/6J mice. Two regions of interest, the AWAY region (away from the loading site) and the NEAR region (near the loading site), were determined. The inflammatory score increased in the NEAR region, but not in the AWAY region. A strain intensity map obtained from \(\upmu \hbox {CT}\) images was superimposed onto the images of the bone formation inhibitor, sclerostin-positive cell localization. The number of sclerostin-positive cells significantly decreased after mechanical loading of more than \(150\,{\upmu }{\upvarepsilon }\) in the AWAY region, but not in the NEAR region. The mineral apposition rate, which shows the bone formation ability of osteoblasts, was accelerated at the site of surface strain intensity, namely around \(170\,{\upmu }{\upvarepsilon }\), but not at the site of lower surface strain intensity, which was around \(80\,{\upmu }{\upvarepsilon }\) in the AWAY region, thus suggesting the existence of a strain intensity threshold for promoting bone formation. Taken together, our data suggest that a threshold of mechanical strain intensity for the direct activation of osteoblast function and the reduction of sclerostin exists in a murine maxilla loading model in the non-inflammatory region.     

Quantitative relationships between lipophilicity and mutagenic effects of N-substituted amides of 3-(5-nitro-2-furyl)-acrylic acid on Salmonella typhimurium

Mutagenic effects of 10 N-alkylamides of 3-(5-nitro-2-furyl)-acrylic acid were assessed in two strains of Salmonella typhimurium TA100 (rfa+ and rfa-). Experimental data were confronted with physiologically based compartment model comprising passive membrane transport, metabolical inactivation in cytoplasm and formation of a reactive intermediate which is responsible for receptor modification. The quantitative dependences observed between mutagenicity and lipophilicity indicate that, in both cases, the drug-receptor interaction takes place in a hydrophobic compartment localized in the cytoplasm. The mutagenic potency of some derivatives was influenced also by stericity.     

High vegetation and environmental diversity during the Late Glacial and Early Holocene on the example of lowlands in the Czech Republic

The diversity of vegetation and the environment in the Late Glacial period in the Elbe region is illustrated by a comparison of three palynological localities. The localities differ in their history, profile lithology and position relative to the Elbe river. The Hrabanovská ?ernava profile holds a record of the development of a shallow lake, which was surrounded by a cold continental steppe in the Early Dryas. Evidence of a pine forest in the Late Glacial period is captured in the surroundings of the profile Chrást. The Mělnický úval — P?ívory locality is an interdune infill, where marl sediments redeposited in shallow water. The surrounding vegetation was diffuse and influenced by erosion. In the Early Holocene, the landscape was covered by an open birch-pine forest. Broad-leaved woody species appeared later. Localities in the Elbe region share a high proportion of Pinus throughout the Holocene as a result of the spread of drift and terrace sands. The human impact in the mid Holocene manifests as evidence of intensive charring of localities.