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排序方式: 共有237条查询结果,搜索用时 11 毫秒
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Vasco M. N. C. S. Vieira Luz Valeria Oppliger Aschwin H. Engelen Juan A. Correa 《PloS one》2015,10(3)
Point 1
Management of crops, commercialized or protected species, plagues or life-cycle evolution are subjects requiring comparisons among different demographic strategies. The simpler methods fail in relating changes in vital rates with changes in population viability whereas more complex methods lack accuracy by neglecting interactions among vital rates.Point 2
The difference between the fitness (evaluated by the population growth rate λ) of two alternative demographies is decomposed into the contributions of the differences between the pair-wised vital rates and their interactions. This is achieved through a full Taylor expansion (i.e. remainder = 0) of the demographic model. The significance of each term is determined by permutation tests under the null hypothesis that all demographies come from the same pool.Point 3
An example is given with periodic demographic matrices of the microscopic haploid phase of two kelp cryptic species observed to partition their niche occupation along the Chilean coast. The method provided clear and synthetic results showing conditional differentiation of reproduction is an important driver for their differences in fitness along the latitudinal temperature gradient. But it also demonstrated that interactions among vital rates cannot be neglected as they compose a significant part of the differences between demographies.Point 4
This method allows researchers to access the effects of multiple effective changes in a life-cycle from only two experiments. Evolutionists can determine with confidence the effective causes for changes in fitness whereas population managers can determine best strategies from simpler experimental designs. 相似文献195.
196.
The growing number of complete sequencing projects based on the next-generation sequencing (NGS) platforms necessitates quality evaluation. Therefore, the use of guaranteed measures such as N50, N80 and average size of contigs etc. to evaluate the quality of genome assemblies produced by ab initio methods remains vital. Herein, we prove that various treatment qualities and their influence on the whole genome products must be considered in genome assembly quality measurements. 相似文献
197.
Lopes T Silva A Thiago R Carneiro A Dorella FA Rocha FS dos Santos AR Lima AR Guimarães LC Barbosa EG Ribeiro D Fiaux KK Diniz CA de Abreu VA de Almeida SS Hassan SS Ali A Bakhtiar SM Aburjaile FF Pinto AC Soares Sde C Pereira Ude P Schneider MP Miyoshi A Edman J Spier S Azevedo V 《Journal of bacteriology》2012,194(13):3567-3568
In this work we report the genome of Corynebacterium pseudotuberculosis strain 267, isolated from a llama. This pathogen is of great veterinary and economic importance, as it is the cause of caseous lymphadenitis in several livestock species around the world and causes significant losses due to the high cost of treatment. 相似文献
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FE Pethick AF Lainson R Yaga A Flockhart DG Smith W Donachie LT Cerdeira A Silva E Bol TS Lopes MS Barbosa AC Pinto AR Dos Santos SC Soares SS Almeida LC Guimaraes FF Aburjaile VA Abreu D Ribeiro KK Fiaux CA Diniz EG Barbosa UP Pereira SS Hassan A Ali SM Bakhtiar FA Dorella AR Carneiro RT Ramos FS Rocha MP Schneider A Miyoshi V Azevedo MC Fontaine 《Journal of bacteriology》2012,194(16):4476
Corynebacterium pseudotuberculosis causes disease in several animal species, although distinct biovars exist that appear to be restricted to specific hosts. In order to facilitate a better understanding of the differences between biovars, we report here the complete genome sequence of the equine pathogen Corynebacterium pseudotuberculosis strain 1/06-A. 相似文献
199.
Vasco Filipe Wim Jiskoot Abdul Hafid Basmeleh Andhyk Halim Huub Schellekens Vera Brinks 《MABS-AUSTIN》2012,4(6):740-752
The presence of protein aggregates in biopharmaceutical formulations is of great concern for safety and efficacy reasons. The aim of this study was to correlate the type and amount of IgG monoclonal antibody aggregates with their immunogenic potential. IgG degradation was obtained by freeze-thawing cycles, pH-shift cycles, heating, shaking and metal-catalyzed oxidation. The size, amount, morphology and type of intermolecular bonds of aggregates, as well as structural changes and epitope integrity were characterized. These formulations were injected in mice transgenic (TG) for human genes for Ig heavy and light chains and their non-transgenic (NTG) counterparts. Anti-drug antibody (ADA) titers were determined by bridging ELISA. Both unstressed IgG and freeze-thawed formulation did not induce measurable ADA levels. A mild antibody response was obtained in a fairly small percentage of mice, when injected with shaken, pH-shifted and heated formulations. The metal-catalyzed oxidized IgG formulation was the most immunogenic one, in both ADA titers and number of responders. The overall titers of NTG responders were significantly higher than the ones produced by TG mice, whereas there was no significant difference between the overall number of TG and NTG responders. This study reinforces the important role of protein aggregates on immunogenicity of therapeutic proteins and provides new insight into the immunogenic potential of different types of IgG aggregates. The results indicate that the quality of the IgG aggregates has more impact on the development of an immune response than their quantity or size. 相似文献
200.
SN Shelke GR Mhaske VD Bonifácio MB Gawande 《Bioorganic & medicinal chemistry letters》2012,22(17):5727-5730
A new series of fluorinated pyrazoles, 4a-e, were synthesized in good to excellent overall yields (65-82%) from the corresponding chalcones, 3a-e, by ultrasonic irradiation. The newly synthesized compounds were characterized and screened for their in vitro anti-bacterial, anti-fungal, and anti-tubercular activities against Mycobacterium tuberculosis H(37)Rv. 相似文献