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181.
Caroline Leonel Isadora F. G. Sena Walison N. Silva Pedro H. D. M. Prazeres Gabriel R. Fernandes Pamela Mancha Agresti Mariana Martins Drumond Akiva Mintz Vasco A. C. Azevedo Alexander Birbrair 《Journal of cellular and molecular medicine》2019,23(9):5949-5955
Wound healing is a complex dynamic physiological process in response to cutaneous destructive stimuli that aims to restore the cutaneous’ barrier role. Deciphering the underlying mechanistic details that contribute to wound healing will create novel therapeutic strategies for skin repair. Recently, by using state‐of‐the‐art technologies, it was revealed that the cutaneous microbiota interact with skin immune cells. Strikingly, commensal Staphylococcus epidermidis‐induced CD8+ T cells induce re‐epithelization of the skin after injury, accelerating wound closure. From a drug development perspective, the microbiota may provide new therapeutic candidate molecules to accelerate skin healing. Here, we summarize and evaluate recent advances in the understanding of the microbiota in the skin microenvironment. 相似文献
182.
Functional studies of mutations in the human protoporphyrinogen oxidase gene in variegate porphyria.
Rhian R Morgan Silva Vasco da Hervé Puy Jean-Charles Deybach George H Elder 《Cellular and molecular biology, including cyto-enzymology》2002,48(1):79-82
The autosomal dominant disorder, variegate porphyria (VP), results from mutations in the protoporphyrinogen oxidase (PPOX) gene. We have investigated the effects of 22 disease-associated missense mutations in this gene on enzyme activity. Mutants were generated in the expression plasmid pHPPOX by site-directed mutagenesis. They were screened for PPOX activity by complementation of the Escherischia coli strain SAS38X which lacks PPOX activity. Ten mutants (G40E, L85P, G232R, de1281H, V282D, L295P, V335G, S350P, L444P, G453V) had no detectable PPOX activity. PPOX activity of the remaining 12 mutants (L15F, R38P, L73P, V84G, D143V, R152C, L154P, V158M, R168H, A172V, V290L, G453R) ranged from less than 1% to 9.2% of wild-type activity. Our findings show that all 22 mutations substantially impair or abolish PPOX activity in a prokaryotic expression system and add to the evidence that they cause VP. 相似文献
183.
Swine and poultry pathogens: the complete genome sequences of two strains of Mycoplasma hyopneumoniae and a strain of Mycoplasma synoviae 总被引:4,自引:0,他引:4
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Vasconcelos AT Ferreira HB Bizarro CV Bonatto SL Carvalho MO Pinto PM Almeida DF Almeida LG Almeida R Alves-Filho L Assunção EN Azevedo VA Bogo MR Brigido MM Brocchi M Burity HA Camargo AA Camargo SS Carepo MS Carraro DM de Mattos Cascardo JC Castro LA Cavalcanti G Chemale G Collevatti RG Cunha CW Dallagiovanna B Dambrós BP Dellagostin OA Falcão C Fantinatti-Garboggini F Felipe MS Fiorentin L Franco GR Freitas NS Frías D Grangeiro TB Grisard EC Guimarães CT Hungria M Jardim SN Krieger MA 《Journal of bacteriology》2005,187(16):5568-5577
This work reports the results of analyses of three complete mycoplasma genomes, a pathogenic (7448) and a nonpathogenic (J) strain of the swine pathogen Mycoplasma hyopneumoniae and a strain of the avian pathogen Mycoplasma synoviae; the genome sizes of the three strains were 920,079 bp, 897,405 bp, and 799,476 bp, respectively. These genomes were compared with other sequenced mycoplasma genomes reported in the literature to examine several aspects of mycoplasma evolution. Strain-specific regions, including integrative and conjugal elements, and genome rearrangements and alterations in adhesin sequences were observed in the M. hyopneumoniae strains, and all of these were potentially related to pathogenicity. Genomic comparisons revealed that reduction in genome size implied loss of redundant metabolic pathways, with maintenance of alternative routes in different species. Horizontal gene transfer was consistently observed between M. synoviae and Mycoplasma gallisepticum. Our analyses indicated a likely transfer event of hemagglutinin-coding DNA sequences from M. gallisepticum to M. synoviae. 相似文献
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185.
Christophe Brunet Raghu Chandrasekaran Lucia Barra Vasco Giovagnetti Federico Corato Alexander V. Ruban 《PloS one》2014,9(1)
Phytoplankton, such as diatoms, experience great variations of photon flux density (PFD) and light spectrum along the marine water column. Diatoms have developed some rapidly-regulated photoprotective mechanisms, such as the xanthophyll cycle activation (XC) and the non-photochemical chlorophyll fluorescence quenching (NPQ), to protect themselves from photooxidative damages caused by excess PFD. In this study, we investigate the role of blue fluence rate in combination with red radiation in shaping photoacclimative and protective responses in the coastal diatom Pseudo-nitzschia multistriata. This diatom was acclimated to four spectral light conditions (blue, red, blue-red, blue-red-green), each of them provided with low and high PFD. Our results reveal that the increase in the XC pool size and the amplitude of NPQ is determined by the blue fluence rate experienced by cells, while cells require sensing red radiation to allow the development of these processes. Variations in the light spectrum and in the blue versus red radiation modulate either the photoprotective capacity, such as the activation of the diadinoxanthin-diatoxanthin xanthophyll cycle, the diadinoxanthin de-epoxidation rate and the capacity of non-photochemical quenching, or the pigment composition of this diatom. We propose that spectral composition of light has a key role on the ability of diatoms to finely balance light harvesting and photoprotective capacity. 相似文献
186.
Rommel Thiago Jucá Ramos Artur Silva Adriana Ribeiro Carneiro Anne Cybelle Pinto Siomar de Castro Soares Anderson Rodrigues Santos Sintia Silva Almeida Luis Carlos Guimar?es Flávia Figueira Aburjaile Eudes Guilherme Vieira Barbosa Fernanda Alves Dorella Flávia Souza Rocha Louise Teixeira Cerdeira Maria Silvanira Barbosa Andreas Tauch Judy Edman Sharon Spier Anderson Miyoshi Maria Paula Cruz Schneider Vasco Azevedo 《Journal of bacteriology》2012,194(23):6620-6621
The bacterium Corynebacterium pseudotuberculosis is of major veterinary importance because it affects livestock, particularly sheep, goats, and horses, in several countries, including Australia, Brazil, the United States, and Canada, resulting in significant economic losses. In the present study, we describe the complete genome of the Corynebacterium pseudotuberculosis Cp316 strain, biovar equi, isolated from the abscess of a North American horse. 相似文献
187.
Renato Portela Salomão Fredy Alvarado Fernanda Baena-Díaz Mario Enrique Favila Luciana Iannuzzi Carolina Nunes Liberal Bráulio A. Santos Gabriel Alfredo Villegas-Guzmán Daniel González-Tokman 《Ecological Entomology》2020,45(4):886-895
1. Urbanisation causes important losses in biodiversity and ecosystem of animals. To test whether these negative effects are preceded by unhealthy individuals in urban populations, we evaluated the effects of urbanisation on the abundance and physical condition of the dung beetle Dichotomius guaribensis, an endemic species of the Brazilian Atlantic Forest. 2. We sampled beetles in nine forest fragments with different urbanisation degrees, embedded in the urban matrix of the city of João Pessoa. Besides testing the abundance in each fragment, we quantified four indicators of physical condition in males and females: body size, body mass, energetic condition, and mite load. 3. Females tended to be larger, and to have less lipid mass and more mites than males. Urbanisation had no effect on beetle abundance, but it negatively affected body mass and lipid content in males, but not females, revealing different sensitivities for both sexes. Body size and mite load were not affected by urbanisation in males or females. Fragment size had no effect on beetle abundance and physical condition in both sexes, suggesting that urbanisation has a greater impact than fragment size on beetle individual condition. 4. In this study, we show that individual deterioration precedes population declines in disturbed environments, and our study opens new insights into the proximate causes that leads to the loss of biodiversity and ecosystem services in urbanised regions. 相似文献
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189.
Joana Gon?alves Luís Vasco Louro Ivone Ribeiro ?ngelo Oliveira Carla Castro 《Reports of Practical Oncology and Radiotherapy》2014,19(5):343-346
Granulocytic sarcomas are rare tumors that can present in innumerous locations; thus there is very little clinical experience with these cases. Therefore there is no consensus on which is the best treatment for patients with this malignancy.The authors present a case of a female with a granulocytic sarcoma of the breast and review the literature for the role of radiotherapy in the management of this clinical entity. 相似文献
190.