全文获取类型
收费全文 | 62493篇 |
免费 | 4979篇 |
国内免费 | 4735篇 |
专业分类
72207篇 |
出版年
2024年 | 147篇 |
2023年 | 836篇 |
2022年 | 1952篇 |
2021年 | 3234篇 |
2020年 | 2219篇 |
2019年 | 2660篇 |
2018年 | 2495篇 |
2017年 | 1919篇 |
2016年 | 2716篇 |
2015年 | 3914篇 |
2014年 | 4689篇 |
2013年 | 4757篇 |
2012年 | 5709篇 |
2011年 | 5141篇 |
2010年 | 3123篇 |
2009年 | 2809篇 |
2008年 | 3181篇 |
2007年 | 2854篇 |
2006年 | 2429篇 |
2005年 | 2044篇 |
2004年 | 1664篇 |
2003年 | 1533篇 |
2002年 | 1174篇 |
2001年 | 1011篇 |
2000年 | 977篇 |
1999年 | 901篇 |
1998年 | 537篇 |
1997年 | 503篇 |
1996年 | 515篇 |
1995年 | 459篇 |
1994年 | 441篇 |
1993年 | 358篇 |
1992年 | 487篇 |
1991年 | 360篇 |
1990年 | 329篇 |
1989年 | 296篇 |
1988年 | 233篇 |
1987年 | 218篇 |
1986年 | 189篇 |
1985年 | 168篇 |
1984年 | 125篇 |
1983年 | 132篇 |
1982年 | 90篇 |
1981年 | 53篇 |
1980年 | 55篇 |
1979年 | 77篇 |
1976年 | 49篇 |
1974年 | 58篇 |
1973年 | 54篇 |
1972年 | 59篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
981.
Yifeng Yang Yingxiu Li Yunlei Hou Mingze Qin Ping Gong Ju Liu Yanfang Zhao 《Bioorganic & medicinal chemistry letters》2019,29(23):126666
A series of novel 4-phenoxyquinoline derivatives containing 3-oxo-3,4-dihydro-quinoxaline moiety were synthesized and evaluated for their antiproliferative activity against five human cancer cell lines (A549, H460, HT-29, MKN-45 and U87MG) in vitro. Most of the tested compounds exhibited more potent inhibitory activities than the positive control foretinib. Compound 1b, 1s and 1t were further examined for their inhibitory activity against c-Met kinase. The most promising compound 1s (with c-Met IC50 value of 1.42 nM) showed remarkable cytotoxicity against A549, H460, HT-29, MKN45 and U87MG cell lines with IC50 values of 0.39 μM, 0.18 μM, 0.38 μM, 0.81 μM, respectively. Their preliminary structure-activity relationships (SARs) study indicated that the replacement of the aromatic ring with the cyclohexane improved their antiproliferative activity. 相似文献
982.
Feng Liu Yalu Cui Fang Yang Zhijue Xu Lin-Tai Da Yan Zhang 《Bioorganic & medicinal chemistry》2019,27(15):3372-3382
Ellagitannin-derived ellagic acid (EA) and colonic metabolite urolithins are functional dietary ingredients for cancer prevention, but the underlying mechanism need elucidation. Mucin-type O-glycosylation, initiated by polypeptide N-acetyl-α-galactosaminyltransferases (ppGalNAc-Ts), fine-tunes multiple biological processes and is closely associated with cancer progression. Herein, we aim to explore how specific tannin-based polyphenols affect tumor behavior of colorectal cancer cells (CRC) by modulating O-glycosylation. Utilizing HPLC-based enzyme assay, we find urolithin D (UroD), EA and gallic acid (GA) potently inhibit ppGalNAc-Ts. In particular, UroD inhibits ppGalNAc-T2 through a peptide/protein-competitive manner with nanomolar affinity. Computational simulations combined with site-directed mutagenesis further support the inhibitors’ mode of action. Moreover, lectin analysis and metabolic labelling reveal that UroD can reduce cell O-glycans but not N-glycans. Transwell experiments prove that UroD inhibits migration and invasion of CRC cells. Our work proves that specific tannin-based polyphenols can potently inhibit ppGalNAc-Ts activity to reduce cell O-glycosylation and lead to lowering the migration and invasion of CRC cells, suggesting that disturbance of mucin-type O-glycosylation is an important mechanism for the function of dietary polyphenols. 相似文献
983.
984.
Organic–inorganic hybrid perovskite solar cells (PSCs) have become a promising candidate in the photovoltaic field due to their high power conversion efficiency and low material cost. However, the development of PSCs is limited by their poor stability under practical conditions in the presence of oxygen, moisture, sunlight, heat, and the current–voltage (I–V) hysteresis. In particular, the hysteretic I–V issue casts doubt on the validity of the photovoltaic performance results that are achieved, making it difficult to evaluate the authentic performance of PSCs. This review article focuses on understanding the I–V hysteresis behavior in PSCs and on exploring the possible reasons leading to this hysteresis phenomenon. The various strategies attempted to suppress the I–V hysteresis in PSCs are summarized, and a brief future recommendation is provided. 相似文献
985.
986.
Huaiyong Luo Manish K. Pandey Aamir W. Khan Jianbin Guo Bei Wu Yan Cai Li Huang Xiaojing Zhou Yuning Chen Weigang Chen Nian Liu Yong Lei Boshou Liao Rajeev K. Varshney Huifang Jiang 《Plant biotechnology journal》2019,17(7):1248-1260
Cultivated peanut (Arachis hypogaea L.) is an important grain legume providing high‐quality cooking oil, rich proteins and other nutrients. Shelling percentage (SP) is the 2nd most important agronomic trait after pod yield and this trait significantly affects the economic value of peanut in the market. Deployment of diagnostic markers through genomics‐assisted breeding (GAB) can accelerate the process of developing improved varieties with enhanced SP. In this context, we deployed the QTL‐seq approach to identify genomic regions and candidate genes controlling SP in a recombinant inbred line population (Yuanza 9102 × Xuzhou 68‐4). Four libraries (two parents and two extreme bulks) were constructed and sequenced, generating 456.89–790.32 million reads and achieving 91.85%–93.18% genome coverage and 14.04–21.37 mean read depth. Comprehensive analysis of two sets of data (Yuanza 9102/two bulks and Xuzhou 68‐4/two bulks) using the QTL‐seq pipeline resulted in discovery of two overlapped genomic regions (2.75 Mb on A09 and 1.1 Mb on B02). Nine candidate genes affected by 10 SNPs with non‐synonymous effects or in UTRs were identified in these regions for SP. Cost‐effective KASP (Kompetitive Allele‐Specific PCR) markers were developed for one SNP from A09 and three SNPs from B02 chromosome. Genotyping of the mapping population with these newly developed KASP markers confirmed the major control and stable expressions of these genomic regions across five environments. The identified candidate genomic regions and genes for SP further provide opportunity for gene cloning and deployment of diagnostic markers in molecular breeding for achieving high SP in improved varieties. 相似文献
987.
Yuan Wang Ren‐Jie Tang Xiyan Yang Xiaojiang Zheng Qiaolin Shao Qing‐Lin Tang Aigen Fu Sheng Luan 《Plant, cell & environment》2019,42(2):673-687
Multiple transporters and channels mediate cation transport across the plasma membrane and tonoplast to regulate ionic homeostasis in plant cells. However, much less is known about the molecular function of transporters that facilitate cation transport in other organelles such as Golgi. We report here that Arabidopsis KEA4, KEA5, and KEA6, members of cation/proton antiporters‐2 (CPA2) superfamily were colocalized with the known Golgi marker, SYP32‐mCherry. Although single kea4,5,6 mutants showed similar phenotype as the wild type under various conditions, kea4/5/6 triple mutants showed hypersensitivity to low pH, high K+, and high Na+ and displayed growth defects in darkness, suggesting that these three KEA‐type transporters function redundantly in controlling etiolated seedling growth and ion homeostasis. Detailed analysis indicated that the kea4/5/6 triple mutant exhibited cell wall biosynthesis defect during the rapid etiolated seedling growth and under high K+/Na+ condition. The cell wall‐derived pectin homogalacturonan (GalA)3 partially suppressed the growth defects and ionic toxicity in the kea4/5/6 triple mutants when grown in the dark but not in the light conditions. Together, these data support the hypothesis that the Golgi‐localized KEAs play key roles in the maintenance of ionic and pH homeostasis, thereby facilitating Golgi function in cell wall biosynthesis during rapid etiolated seedling growth and in coping with high K+/Na+ stress. 相似文献
988.
最新研究表明,RNA之间可以通过竞争结合共同的microRNA反应元件(microRNA response element, MRE)实现相互调节,这种调控模式构成竞争性内源RNA(Competing endogenous RNA, ceRNA)。已发现的ceRNA包括蛋白编码mRNA和非编码RNA,其中后者包括假基因转录物、长链非编码RNA(Long non-coding RNA, lncRNA)、环状RNA(Circular RNA, circRNA)等。文章主要从ceRNA分类的角度,阐述各类ceRNA构成的调控网络发挥的生物学功能在病理和生理相关过程中的作用,以及可能影响ceRNA调控有效性的因素。 相似文献
989.
Si Zhang Zhongnan Yin Fei-Fei Dai Hao Wang Meng-Jiao Zhou Ming-Hui Yang Shu-Feng Zhang Zhi-Feng Fu Ying-Wu Mei Ming-Xi Zang Lixiang Xue 《Journal of cellular physiology》2019,234(8):13252-13262
Although cardiac hypertrophy is widely recognized as a risk factor that leads to cardiac dysfunction and, ultimately, heart failure, the complex mechanisms underlying cardiac hypertrophy remain incompletely characterized. The nuclear receptor peroxisome proliferator-activated receptor δ (PPARδ) is involved in the regulation of cardiac lipid metabolism. Here, we describe a novel PPARδ-dependent molecular cascade involving microRNA-29a (miR-29a) and atrial natriuretic factor (ANF), which is reactivated in cardiac hypertrophy. In addition, we identify a novel role of miR-29a, in which it has a cardioprotective function in isoproterenol hydrochloride-induced cardiac hypertrophy by targeting PPARδ and downregulating ANF. Finally, we provide evidence that miR-29a reduces the isoproterenol hydrochloride-induced cardiac hypertrophy response, thereby underlining the potential clinical relevance of miR-29a in which it may serve as a potent therapeutic target for heart hypertrophy treatment. 相似文献
990.