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21.
The large-scale N-glycosylation analysis is critical for biomedical research, since a variety of diseases are found to be associated with glycoproteins. By a combination of glycoprotein analysis in insoluble protein fraction solubilized with 1% v/v 1-butyl-3-methylimidazolium tetrafluoroborate (BMIM BF(4)) and those in soluble fraction, a total number of 462 non-redundant N-glycoprotein groups, including 316 transmembrane glycoproteins, were successfully identified. Correspondingly, 849 unique N-glycosites were confidently recognized. The data set could provide a support for the further in-depth research of brain N-glycosylation, such as for the discovery of candidate drug targets and biomarkers. 相似文献
22.
Jiayang He Zhiqiang Wu Liangliang Huang Yangyan Sun Dongjie Wang Yong Lin Anyou He Jie Feng Hao Liu 《Journal of fish biology》2024,104(2):399-409
The age, growth, reproduction and resource development status of Ptychidio jordani, as a critically endangered freshwater fish in the Hongshui River, China, was studied in this work. A total of 525 specimens were collected monthly using the cages and gillnets from October 2021 to September 2022 in the Hongshui River. The scale was used for age determination, and the maximum age for both female and male was estimated to be 5 years and 3 years, respectively. Female and male P. jordani showed different growth patterns, which were expressed as Lt = 261.3 (1-e−0.4885(t−0.1476)) and Lt = 251.2 (1-e−0.4758(t+0.9643)), respectively. The overall sex ratio was 1:0.47 (female:male). Female attained sex maturity at 2.34 years (192 mm body length). Month variation of the gonad somatic index indicated that the spawning period occurred from April to October. The absolute fecundity was estimated at 9046 ± 3434 eggs per individual, and the relative fecundity was 38.08 ± 15.77 eggs per gram. The exploitation rate of female and male was 0.233 and 0.495, which indicated that P. jordani was not overfishing. This study provided data on the key life-history traits of P. jordani, which has not been known previously and is essential for conservation strategy and policy development. 相似文献
23.
Jianhua Li Liangliang Huang Limin Zou Yuichi Kano Tatsuro Sato Tetsukazu Yahara 《Environmental Biology of Fishes》2012,93(3):403-417
The spatial and temporal variations of the fish assemblages in mountain streams of China are poorly understood. The relationships
between the fish assemblage and selected habitat features were examined in the North Tiaoxi River, one of headwaters of Taihu
Lake. A total of 3,348 individuals belonging to 5 orders, 11 families, 25 genera and 34 species were collected including 33
native species and one invasive species. Among those, about 20 species were endemic to China. Non-metric Multidimensional
Scaling (NMDS) was applied to compare fish assemblage structures from upstream to downstream during four seasons. Species
assemblages differed along the stream continuum, but there was little apparent change associated with the seasons. Species
richness and Shannon-Weaver index (H′) tended to increase along the stream continuum from the upstream to downstream and the proportion of invertivorous fish tended
to significantly decrease along the continuum with a parallel significant increase in the percentage of omnivores. Fish assemblages
were significantly related to both water quality and habitat structure variables. Canonical Correspondence Analysis ordinations
(CCA) revealed that 6 of the 14 selected environmental variables had significant relationships with the fish assemblage such
as distance to source, stream width, altitude, pH, water depth, and water velocity and different sampling sites were associated
with different environmental variables in different seasons. The main differences in fish assemblage structure and diversity
within the whole watercourse are probably related to large-scale factors such distance to source, altitude and stream width.
Differences of instream characteristics are likely to be caused by natural variability of the ecosystems but also, in some
case, by anthropogenic influence like human settlements, agriculture and river embankment and pollution from small factory. 相似文献
24.
Legumes are widely used in many cropping systems because they share their nitrogen fixation products and phosphorus mobilization activities with their neighbors. In the current study, however, we showed that co-cultivation with legumes increased cadmium (Cd) contamination in the adjacent crops. Both field and mesocosm experiments indicated that legumes increased Cd levels in edible parts and shoots of four neighboring crops and five maize varieties tested, regardless of the Cd levels in the soil. This enhanced Cd accumulation in crops was attributed to root interactions that alter the rhizosphere environment. Co-cultivation with legumes reduced soil pH, which somewhat increased the exchangeable forms of Cd. Our results have demonstrated the inevitable increases in Cd levels of crops as a direct result of co-cultivation with legumes even under situations when these levels are below the permissible threshold. With this new revelation, we need to consider carefully the current cropping systems involving legumes and perhaps to re-design the current and future cropping systems in view of avoiding food contamination by Cd. 相似文献
25.
Liangliang Sun Yanyan Zhang Dingyin Tao Guijie Zhu Qun Zhao Qi Wu Zhen Liang Ling Yang Lihua Zhang Yukui Zhang 《Proteomics》2012,12(23-24):3464-3469
Comprehensive identification of cytochrome P450 enzymes (CYPs) and uridine diphosphoglucuronosyl transferases (UGTs) in human liver microsomes (HLMs) was performed with an SDS‐PAGE‐free protocol. HLMs were solubilized with 5% v/v ionic liquid, 1‐butyl‐3‐methyl imidazolium tetrafluoroborate, followed by tryptic digestion, and 2D‐SCX‐RPLC‐ESI‐MS/MS (LTQ XL) analysis in triplicate. In total, 27 CYPs and 12 UGTs were confidently identified with average sequence coverage as 30.99 and 25.07%, average peptide number as 14 and 13, and average unique peptide number as 7 and 4, respectively. The highly similar isoforms of CYP3A, CYP2C, and CYP4F subfamilies could be unambiguously differentiated from each other, despite the fact that the sequence similarity of CYP2C9 and CYP2C19 is 91%. In addition, protein spectral count was used to approximately evaluate the relative abundance of identified CYPs and UGTs, and the results agreed with previous immunochemistry reports. 相似文献
26.
Huiling Duan Liangliang Tang Yuan Zheng Ping Zhang 《Plasmonics (Norwell, Mass.)》2018,13(4):1135-1141
The plasmonic effect is introduced in solar thermal areas to enhance light harvest and absorption. The optical properties of plasmonic nanofluid are simulated by finite difference time domain (FDTD) method. Due to the excitation of localized surface plasmon resonance (LSPR) effect, an intensive absorption peak is observed at 0.5 μm. The absorption characteristics are sensitive to particle size and concentration. As the particle size increases, the absorption peak is broadened and shifted to longer wavelength. The absorption of SiO2/Ag plasmonic nanofluid is improved gradually as the volume concentration increases, especially in the UV region. The absorption edge is shifted from 0.6 to 1.0 μm as the volume concentration increases from 0.001 to 0.01. The thermal simulation of suspended SiO2/Ag nanoparticle shows a uniform temperature rise of 17.91 K under solar irradiation (AM 1.5), while under the same condition, the temperature rises in Ag nanoparticle and Al nanoparticle are 11.12 and 5.39 K, respectively. The core/shell plasmonic nanofluid exhibits a higher photothermal performance, which has a potential application in photothermal areas. A higher temperature rise can be obtained by improving the incident light intensity or optical absorption properties of nanoparticles. 相似文献
27.
Solar Absorber Gel: Localized Macro‐Nano Heat Channeling for Efficient Plasmonic Au Nanoflowers Photothermic Vaporization and Triboelectric Generation
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Minmin Gao Connor Kangnuo Peh Huy Thong Phan Liangliang Zhu Ghim Wei Ho 《Liver Transplantation》2018,8(25)
Plasmonic nanoparticles with outstanding photothermal conversion efficiency are promising for solar vaporization. However, the high cost and the required intense light excitation of noble metals, hinder their practical application. Herein, an inexpensive 3D plasmonic solar absorber gel that embraces all the desirable optical, thermal, and wetting properties for efficient solar vaporization is reported. The broadband absorption and strong near‐field intertip enhancement of the sparsely dispersed gold nanoflowers contribute to efficient light‐to‐heat conversion, while the macro‐nano thermal insulative silica gel retains and channels the plasmonic heat directly to the water pathways contained within the porous gel. The plasmonic‐based solar absorber gel shows a vaporization efficiency of 85% under solar irradiation of 1 sun intensity (1 kW m?2). Moreover, the porous gel framework exhibits high mechanical stability and antifouling properties, potentially useful for polluted/turbid water evaporation. Complementary water condensation‐induced triboelectricity can be harvested alongside fresh water condensate, granting simultaneous fresh water production and electricity generation functionalities. The facile sol‐gel synthesis at room temperature makes the solar absorber gel highly adaptable for practical large‐scale photothermal applications. 相似文献
28.
Systematic Evaluation of Immobilized Trypsin‐Based Fast Protein Digestion for Deep and High‐Throughput Bottom‐Up Proteomics
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Immobilized trypsin (IM) has been recognized as an alternative to free trypsin (FT) for accelerating protein digestion 30 years ago. However, some questions of IM still need to be answered. How does the solid matrix of IM influence its preference for protein cleavage and how well can IM perform for deep bottom‐up proteomics compared to FT? By analyzing Escherichia coli proteome samples digested with amine or carboxyl functionalized magnetic bead–based IM (IM‐N or IM‐C) or FT, it is observed that IM‐N with the nearly neutral solid matrix, IM‐C with the negatively charged solid matrix, and FT have similar cleavage preference considering the microenvironment surrounding the cleavage sites. IM‐N (15 min) and FT (12 h) both approach 9000 protein identifications (IDs) from a mouse brain proteome. Compared to FT, IM‐N has no bias in the digestion of proteins that are involved in various biological processes, are located in different components of cells, have diverse functions, and are expressed in varying abundance. A high‐throughput bottom‐up proteomics workflow comprising IM‐N‐based rapid protein cleavage and fast CZE‐MS/MS enables the completion of protein sample preparation, CZE‐MS/MS analysis, and data analysis in only 3 h, resulting in 1000 protein IDs from the mouse brain proteome. 相似文献
29.
Solar Absorber Gel: Solar Absorber Gel: Localized Macro‐Nano Heat Channeling for Efficient Plasmonic Au Nanoflowers Photothermic Vaporization and Triboelectric Generation (Adv. Energy Mater. 25/2018)
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30.
Chaowen Zhang Feifan Chen Ziyao Zhao Liangliang Hu Hanqiang Liu Zhihui Cheng Yiqun Weng Peng Chen Yuhong Li 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2018,131(6):1379-1389