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81.
82.
Acetylcholine Inhibits Tumor Necrosis Factor α Activated Endoplasmic Reticulum Apoptotic Pathway via EGFR‐PI3K Signaling in Cardiomyocytes 下载免费PDF全文
83.
Septins are conserved GTP-binding proteins that assemble into hetero-oligomeric complexes and higher-order structures such as filaments, rings, hourglasses or gauzes. Septins are usually associated with a discrete region of the plasma membrane and function as a cell scaffold or diffusion barrier to effect cytokinesis, cell polarity, and many other functions. Recent structural studies of septin complexes have provided mechanistic insights into septin filament assembly, but key questions concerning the assembly, dynamics, and function of different septin structures remain to be answered. 相似文献
84.
Jia Tong HengXi Wei XiaoFang Liu WenPing Hu MingJun Bi YuanYuan Wang QiuYan Li Ning Li 《Transgenic research》2011,20(2):417-419
In the swine industry pathogenic infections have a significant negative impact on neonatal survival. Piglets fed with human
lysozyme, a natural antibiotic, might be more resistant to gastrointestinal infections. Here we describe the generation of
transgenic swine expressing recombinant human lysozyme by somatic cell nuclear transfer. Three cloned female pigs were born,
one of which expressed rhLZ at 0.32 ± 0.01 μg/ml in milk, 50-fold higher than that of the pig native lysozyme. Both the transgenic
gilts and their progeny appear healthy. Introducing human lysozyme into pigs’ milk has a potential to benefit the piglets
by enhancing immune function and defending against pathogenic bacteria, thereby increasing the new born survival rate. This
advance could be of great value to commercial swine producers. 相似文献
85.
86.
Automated powder dispensing systems enable supplying early clinical studies using drug-in-capsule approach, which is material
sparing and requires a minimum amount of resources. However, the inability of accurately filling the capsule with a small
amount, e.g., several micrograms, of drug limits the use of these systems for potent drugs. We demonstrate that formulated
powder blends can be used to successfully fill capsules containing 5 μg to 5 mg of drug with adequate content uniformity.
Effective formulation and process strategies that enable this approach are presented with examples. 相似文献
87.
Enhancing and sustaining AMG 009 dissolution from a matrix tablet via microenvironmental pH modulation and supersaturation,
where poorly soluble acidic AMG 009 molecule was intimately mixed and compressed together with a basic pH modifier (e.g., sodium carbonate) and nucleation inhibitor hydroxypropyl methylcellulose K100 LV (HPMC K100 LV), was demonstrated previously. However, not all acidic or basic drugs are compatible with basic or acidic pH
modifiers either chemically or physically. The objective of this study is to investigate whether similar dissolution enhancement
of AMG 009 can be achieved from a bilayer dosage form, where AMG 009 and sodium carbonate are placed in a separate layer with
or without the addition of HPMC K100 LV in each layer. Study results indicate that HPMC K100 LV-containing bilayer dosage
forms gained similar dissolution enhancement as matrix dosage forms did. Bilayer dosage forms without HPMC K100 LV benefitted
the least from dissolution enhancement. 相似文献
88.
The complete mitochondrial genome of Alosa sapidissima has been determined. The total length of the mitogenome was 16,697 bp and had a gene content (13 protein-coding, 22 tRNAs and 2 rRNAs. Except for the seven tRNA and Nd6 genes, all other mitochondrial genes are encoded on the heavy strand. The overall base composition of the heavy strand is 28.3% A, 24.8% T, 28.9% C, 17.9% G, with an AT content of 53.1%. The DNA sequence of Alosa. sapidissima shared 97.1, 93.9, 88.8 and 82.3% sequence identity with that of Alosa alosa, Alosa pseudoharengus. Molecular data here presented provide a useful toll for evolutionary as well as population genetic studied. 相似文献
89.
In eukaryotic cells, repair of DNA double-strand breaks (DSBs) by the nonhomologous end-joining (NHEJ) pathway is critical for genome stability. In contrast to the complex eukaryotic repair system, bacterial NHEJ apparatus consists of only two proteins, Ku and a multifunctional DNA ligase (LigD), whose functional mechanism has not been fully clarified. We show here for the first time that Sir2 is involved in the mycobacterial NHEJ repair pathway. Here, using tandem affinity purification (TAP) screening, we have identified an NAD-dependent deacetylase in mycobacteria which is a homologue of the eukaryotic Sir2 protein and interacts directly with Ku. Results from an in vitro glutathione S-transferase (GST) pull-down assay suggest that Sir2 interacts directly with LigD. Plasmid-based end-joining assays revealed that the efficiency of DSB repair in a sir2 deletion mutant was reduced 2-fold. Moreover, the Δsir2 strain was about 10-fold more sensitive to ionizing radiation (IR) in the stationary phase than the wild-type. Our results suggest that Sir2 may function closely together with Ku and LigD in the nonhomologous end-joining pathway in mycobacteria. 相似文献
90.
A genome-wide association study confirms previously reported loci for type 2 diabetes in Han Chinese
Cui B Zhu X Xu M Guo T Zhu D Chen G Li X Xu L Bi Y Chen Y Xu Y Li X Wang W Wang H Huang W Ning G 《PloS one》2011,6(7):e22353