全文获取类型
收费全文 | 49819篇 |
免费 | 17204篇 |
国内免费 | 2425篇 |
出版年
2024年 | 44篇 |
2023年 | 296篇 |
2022年 | 719篇 |
2021年 | 1697篇 |
2020年 | 3066篇 |
2019年 | 4721篇 |
2018年 | 4736篇 |
2017年 | 4757篇 |
2016年 | 5024篇 |
2015年 | 5440篇 |
2014年 | 5349篇 |
2013年 | 5956篇 |
2012年 | 4130篇 |
2011年 | 3511篇 |
2010年 | 4260篇 |
2009年 | 2937篇 |
2008年 | 1956篇 |
2007年 | 1421篇 |
2006年 | 1234篇 |
2005年 | 1181篇 |
2004年 | 988篇 |
2003年 | 956篇 |
2002年 | 835篇 |
2001年 | 721篇 |
2000年 | 568篇 |
1999年 | 535篇 |
1998年 | 260篇 |
1997年 | 235篇 |
1996年 | 216篇 |
1995年 | 182篇 |
1994年 | 167篇 |
1993年 | 138篇 |
1992年 | 172篇 |
1991年 | 130篇 |
1990年 | 156篇 |
1989年 | 118篇 |
1988年 | 89篇 |
1987年 | 75篇 |
1986年 | 68篇 |
1985年 | 67篇 |
1984年 | 49篇 |
1983年 | 35篇 |
1982年 | 34篇 |
1981年 | 22篇 |
1980年 | 16篇 |
1979年 | 32篇 |
1978年 | 14篇 |
1977年 | 17篇 |
1973年 | 18篇 |
1971年 | 14篇 |
排序方式: 共有10000条查询结果,搜索用时 171 毫秒
101.
Xiaoqing Wu Xiaorui Xie Linjuan Su Na Lin Bin Liang Nan Guo Qingquan Chen Liangpu Xu Hailong Huang 《Journal of cellular and molecular medicine》2021,25(18):8929-8935
Pallister-Killian syndrome (PKS) is a rare sporadic genetic disorder usually caused by mosaicism of an extra isochromosome of 12p (i(12p)). This retrospective study analysed the prenatal ultrasound manifestations and molecular and cytogenetic results of five PKS foetuses. Samples of amniotic fluid and/or cord blood, skin biopsy and placenta were collected. Conventional karyotyping and single nucleotide polymorphism array (SNP array) were performed on all the amniotic fluid or cord blood samples. Copy number variants sequencing (CNV-seq) and fluorescence in situ hybridization (FISH) were also used for the validation for one foetus. All the five foetuses were from pregnancies with advanced parental age. Two foetuses involved structural abnormalities and one foetus had only soft markers, all of which included increased nuchal translucency. The rest two foetuses had normal ultrasounds in the second trimester, which has rarely been reported before. The karyotype revealed typical i(12p) in four cases and a small supernumerary marker chromosome consisting of 12p and 20p in the remaining one case. The proportion of cells with i(12p) ranged from 0 to 100% in cultural cells, while SNP array results suggested 2−4 copies of 12p. For one foetus, metaphase FISH showed normal results, but the interphase FISH suggested cell lines with two, three and four copies of 12p in the amniotic fluid. Advanced parental age may be an important risk factor for PKS, and there were no typical ultrasound manifestations related to PKS. A combination of karyotype analysis and molecular diagnosis is an effective method for the diagnosis of PKS. 相似文献
102.
T. RIGOT M. VERCAUTEREN DRUBBEL J.‐C. DELÉCOLLE M. GILBERT 《Medical and veterinary entomology》2013,27(1):29-38
The spatial epidemiology of Bluetongue virus (BTV) at the landscape level relates to the fine‐scale distribution and dispersal capacities of its vectors, midges belonging to the genus Culicoides Latreille (Diptera: Ceratopogonidae). Although many previous researches have carried out Culicoides sampling on farms, little is known of the fine‐scale distribution of Culicoides in the landscape immediately surrounding farms. The aim of this study was to gain a better understanding of Culicoides populations at increasing distances from typical dairy farms in north‐west Europe, through the use of eight Onderstepoort‐type black‐light traps positioned along linear transects departing from farms, going through pastures and entering woodlands. A total of 16 902 Culicoides were collected in autumn 2008 and spring 2009. The majority were females, of which more than 97% were recognized as potential vectors. In pastures, we found decreasing numbers of female Culicoides as a function of the distance to the farm. This pattern was modelled by leptokurtic models, with parameters depending on season and species. By contrast, the low number of male Culicoides caught were homogeneously distributed along the transects. When transects entered woodlands, we found a higher abundance of Culicoides than expected considering the distance of the sampling sites to the farm, although this varied according to species. 相似文献
103.
104.
105.
106.
Morgan Sheng Michael Wyszynski 《BioEssays : news and reviews in molecular, cellular and developmental biology》1997,19(10):847-853
Electrical signaling by neurons depends on the precisely ordered distribution of a wide variety of ion channels on the neuronal surface. The mechanisms underlying the targeting of particular classes of ion channels to specific subcellular sites are poorly understood. Recent studies have identified a new class of protein-protein interaction mediated by PDZ domains, protein binding modules that recognize specific sequences at the C terminus of membrane proteins. The PDZ domains of a family of synaptic cytoskeleton-associated proteins, typified by PSD-95, bind to the intracellular C-terminal tails of NMDA receptors and Shaker-type K+ channels. This interaction appears to be important in the clustering and localization of these ion channels at synaptic sites. Recognition of specific C-terminal peptide sequences by different PDZ domain-containing proteins may be a general mechanism for differential targeting of proteins to a variety of subcellular locations. 相似文献
107.
108.
109.
Hanhan Liu Qiangqiang Jia Gianluca Tettamanti Sheng Li 《Insect biochemistry and molecular biology》2013,43(11):1068-1078
In the fruitfly, Drosophila melanogaster, autophagy and caspase activity function in parallel in the salivary gland during metamorphosis and in a common regulatory hierarchy during oogenesis. Both autophagy and caspase activity progressively increase in the remodeling fat body, and they are induced by a pulse of the molting hormone (20-hydroxyecdysone, 20E) during the larval-prepupal transition. Inhibition of autophagy and/or caspase activity in the remodeling fat body results in 25–40% pupal lethality, depending on the genotypes. Interestingly, a balancing crosstalk occurs between autophagy and caspase activity in this tissue: the inhibition of autophagy induces caspase activity and the inhibition of caspases induces autophagy. The Drosophila remodeling fat body provides an in vivo model for understanding the molecular mechanism of the balancing crosstalk between autophagy and caspase activity, which oppose with each other and are induced by the common stimulus 20E, and blockage of either path reinforces the other path. 相似文献
110.
Distinct effects of climate warming on populations of silver fir (Abies alba) across Europe
下载免费PDF全文
![点击此处可从《Journal of Biogeography》网站下载免费的PDF全文](/ch/ext_images/free.gif)