Traditional cultural landscape in Viñales,Cuba

Biodiversity and Conservation - The Viñales Valley has a wide range of biological and cultural values thanks to both its landscape diversity and the use of low-impact agricultural practices....     

DNA-diagnosis of sickle cell anemia from chorionic villi: possible influence of maternal cell contamination

Summary The admixture of maternal tissue is a possible hazard of prenatal diagnosis from chorionic villi. Therefore the tolerable degree of maternal DNA contamination in prenatal diagnosis of sickle cell anemia was investigated by mixing various amounts of DNA from HbS carriers with DNA from normal individuals and sickle cell anemia patients. The results indicate that lower rate of admixed maternal DNA does not prevent an exact direct DNA-diagnosis of sickle cell anemia.The experimental part of this paper was performed at the Abteilung Humangenetik, Universität Ulm, D-7900 Ulm, Federal Republic of Germany     

Effect of substance P on the ganglion trigeminale in tissue culture (author's transl)

Explants of the ganglion trigeminale from chick embryos were cultivated in Maximow chambers in the presence of 10-6...10-8 M substance P (SP . 3CH3COOH.4H2O). 1. In SP-treated cultures the index of areas covered by the explants was increased in shorttime tests. 2. The density of cells was related to the type of medio-dorsal (MD) and ventro-lateral (VL) neuroblasts. The density of SP-treated VL cells was not altered. The density of MD cells decreased. 3. The percentage of dark neuroblasts was decreased under the influence of SP. 4. A stimulation of VL neuroblasts did not take place. 5. The diameters of MD pericarya and the areas of MD cell nuclei and the areas of nuclei from nonneuronal cells increased. 6. The possible role of SP as a factor controlling In-vitro-processes is discussed.