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121.
Clonal isolation of muscle-derived cells capable of enhancing muscle regeneration and bone healing 总被引:44,自引:0,他引:44
Lee JY Qu-Petersen Z Cao B Kimura S Jankowski R Cummins J Usas A Gates C Robbins P Wernig A Huard J 《The Journal of cell biology》2000,150(5):1085-1100
Several recent studies suggest the isolation of stem cells in skeletal muscle, but the functional properties of these muscle-derived stem cells is still unclear. In the present study, we report the purification of muscle-derived stem cells from the mdx mouse, an animal model for Duchenne muscular dystrophy. We show that enrichment of desmin(+) cells using the preplate technique from mouse primary muscle cell culture also enriches a cell population expressing CD34 and Bcl-2. The CD34(+) cells and Bcl-2(+) cells were found to reside within the basal lamina, where satellite cells are normally found. Clonal isolation and characterization from this CD34(+)Bcl-2(+) enriched population yielded a putative muscle-derived stem cell, mc13, that is capable of differentiating into both myogenic and osteogenic lineage in vitro and in vivo. The mc13 cells are c-kit and CD45 negative and express: desmin, c-met and MNF, three markers expressed in early myogenic progenitors; Flk-1, a mouse homologue of KDR recently identified in humans as a key marker in hematopoietic cells with stem cell-like characteristics; and Sca-1, a marker for both skeletal muscle and hematopoietic stem cells. Intramuscular, and more importantly, intravenous injection of mc13 cells result in muscle regeneration and partial restoration of dystrophin in mdx mice. Transplantation of mc13 cells engineered to secrete osteogenic protein differentiate in osteogenic lineage and accelerate healing of a skull defect in SCID mice. Taken together, these results suggest the isolation of a population of muscle-derived stem cells capable of improving both muscle regeneration and bone healing. 相似文献
122.
β-葡萄糖苷酶的分离纯化和性质研究 总被引:12,自引:0,他引:12
β-葡萄糖苷酶是纤维素酶的重要组分之一,它不仅可水解纤维二糖和寡糖,更可解除纤维二糖对β-1,4-内切葡聚糖酶和外切葡聚糖酶的抑制,提高水解速率和程度.利用SephadexG-150和DEAE-SephadexA-50层析法从黑曲霉变异株L-22中分离提纯了β-葡萄糖苷酶,该酶是由两个分子量相同的亚基组成的二聚体,每个亚基分子量为203kD.该酶最适pH为4.8,pH稳定范围在3.6~6.4;最适温度是60℃,温度稳定范围为4~60℃;酶分子含糖量为8.35%.它是一个酸性β-葡萄糖苷水解酶,专一性地水解β-糖苷键.而不水解α-糖苷键,对短链底物表现了相对高的活力.用动力学分析和共价化学修饰方法探讨了与该酶活力有关的必需基团.由pH对lgVm和lgVm/Km的影响,推测出酶活性部位至少有两个可解离基团为酶活性所必需,它们在酶-底物复合物中的pKes1和pKes2的值分别为4.0和5.6,在游离酶中的pK值分别为4.2和5.9.由此可初步判断这两个可解离基团可能为组氨酸和含羧基的氨基酸,它们与酶的催化和底物结合可能有关. 相似文献
123.
Yan Cao Jin Wu Tao Meng Jun Zhang Jiasong He Huiquan Li Yi Zhang 《Carbohydrate polymers》2007,69(4):665-672
Cellulose samples extracted from cornhusk have been successfully acetylated in an ionic liquid 1-allyl-3-methylimidazolium chloride (AmimCl). Without using any catalyst, cornhusk cellulose acetates (CCAs) with the degree of substitution (DS) in a range from 2.16 to 2.63 were prepared in one-step. Under the homogeneous state, the DS value of CCAs was easily controlled by the acetylation time. The obtained CCAs were characterized by means of FT-IR, 13C NMR, DSC, TGA, and a mechanical test. The NMR results showed that the distribution of the acetyl moiety among the three OH groups of the anhydroglucose unit shows a preference at the C6 position. The CCAs exhibited good solubility in some organic solvents, such as acetone and DMSO. The cast CCA films from their acetone solutions had good mechanical properties. At the end of each acetylation of cornhusk cellulose, the ionic liquid AmimCl could be effectively recovered. Therefore, this study presents a promising approach and “green process” to make use of crop by-products. 相似文献
124.
The effect of phenolic compounds on hydrothermal oxidation of cellulose was studied using a batch reactor at 300 degrees C with H(2)O(2) as oxidant. Intermediate products, as well as the yields of acetic acid produced in the oxidation of cellulose, phenolic compounds, and cellulose-phenolic compound mixtures were examined. Phenolic compounds used were phenol, 1,4-benzenediol, 2-methoxy-4-methylphenol, and 2,6-di-tert-butyl-4-methylphenol. In the case of oxidation of cellulose-phenolic compound mixtures, (1) formic acid, a basic oxidation product from carbohydrates, decreased considerably, (2) 5-hydroxymethyl-2-furaldehyde and 2-furaldehyde, acid-catalyzed dehydration products from carbohydrates, appeared, and (3) the yield of acetic acid increased compared to that in the oxidation of cellulose. From these results, phenolic compounds seem to inhibit the oxidation of cellulose under hydrothermal conditions. The inhibition of the oxidation of cellulose by phenolic compounds seems to be related closer to the stability of phenolic compounds under oxidation conditions rather than the ease to remove phenolic hydrogen on the OH group. 相似文献
125.
丁酸钠对CHO-EPO工程细胞株rhEPO表达量的影响 总被引:1,自引:0,他引:1
以稳定整合有pEDEPO的CHOEPO工程细胞株为研究对象,在无血清条件下,系统观察了05、10、25和50mmol/L4个浓度的丁酸钠作用于该细胞株的情况,结果表明:丁酸钠对CHOEPO工程细胞的生长有明显的抑制作用;影响CHOEPO工程细胞EPO表达,浓度10mmol/L可提高EPO表达量25倍左右,并可持续较长的一段时间;延缓CHOEPO工程细胞在无血清培养时的细胞脱落;提高CHOEPO工程细胞EPOmRNA水平 相似文献
126.
127.
Biodiesel production from high acid value waste frying oil catalyzed by superacid heteropolyacid 总被引:2,自引:0,他引:2
Cao F Chen Y Zhai F Li J Wang J Wang X Wang S Zhu W 《Biotechnology and bioengineering》2008,101(1):93-100
Transesterification of waste cooking oil with high acid value and high water contents using heteropolyacid H3PW12O40 x 6H2O (PW12) as catalyst was investigated. The hexahydrate form of PW(12) was found to be the most promising catalyst which exhibited highest ester yield 87% for transesterification of waste cooking oil and ester yield 97% for esterification of long-chain palmitic acid, respectively. The PW12 acid catalyst shows higher activity under the optimized reaction conditions compared with conventional homogeneous catalyst sulfuric acid, and can easily be separated from the products by distillation of the excess methanol and can be reused more times. The most important feature of this catalyst is that the catalytic activity is not affected by the content of free fatty acids (FFAs) and the content of water in the waste cooking oil and the transesterification can occur at a lower temperature (65 degrees C), a lower methanol oil ratio (70:1) and be finished within a shorter time. The results illustrate that PW12 acid is an excellent water-tolerant and environmentally benign acid catalyst for production of biodiesel from waste cooking oil. 相似文献
128.
129.
氮磷对污水净化中藻类叶绿素含量的影响 总被引:2,自引:1,他引:2
在室内模拟生物净化槽中比较研究了氮磷对污水中藻类叶绿素含量和污水净化的影响。污水中磷含量均为7mg/L左右,氮含量分别为77.4、44.4、24.8和13.5mg/L,结果发现TN/TP=77.4/7.01mg/L组,污水经7d净化,藻类叶绿素含量最高,污水净化效果较好。四个实验组比较,叶绿素含量随TN/TP比例的上升而上升,呈显著正相关。 相似文献
130.
Litter species traits, but not richness, contribute to carbon and nitrogen dynamics in an alpine meadow on the Tibetan Plateau 总被引:2,自引:0,他引:2
Jing Jiang Yikang Li Mozhu Wang Caiping Zhou Guangmin Cao Peili Shi Minghua Song 《Plant and Soil》2013,373(1-2):931-941