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541.
Mori Izumi C. Arias-Barreiro Carlos Raul Ooi Lia Lee Nam-Hee Sobahan Muhammad Abdus Nakamura Yoshimasa Hirai Yoshihiko Murata Yoshiyuki 《Journal of plant research》2021,134(5):1139-1148
Journal of Plant Research - It is known that rice roots take up cadmium (Cd) via the symplastic route mediated by membrane-bound mineral transporters. Here we provide evidence that apoplastic... 相似文献
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Verónica V. Lia Lidia Poggio Viviana A. Confalonieri 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2009,119(6):1053-1067
The highland region or Northwestern Argentina (NWA) is one of the southernmost areas of native maize cultivation and constitutes
an expansion of the peruvian Andes sphere of influence. To examine the genetic diversity and racial affiliations of the landraces
cultivated in this area, 18 microsatellite markers were used to characterize 147 individuals from 6 maize races representative
of traditional materials. For the whole data set, a total of 184 alleles were found, with an average of 10.2 alleles per locus.
The average gene diversity was 0.571. The observed patterns of genetic differentiation suggest that historical association
is probably the main factor in shaping population structure for the landraces studied here. In agreement with morphological
and cytogenetic data, Bayesian analysis of NWA landraces revealed the occurrence of three main gene pools. Assessment of racial
affiliations using a combined dataset including previous data on American landraces showed a clear relationship between one
of these gene pools and typical Andean races, whereas the remaining two gene pools exhibited a closer association to Caribbean
accessions and native germplasm from the United States, respectively. These results highlight the importance of integrating
regional genetic studies if a deeper understanding of maize diversification and dispersal is to be achieved. 相似文献
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T. M. Arme R. P. Lia G. Annoscia E. Casalino M. Pombi D. Otranto 《Medical and veterinary entomology》2020,34(2):201-206
Knowledge of the effects that Thelazia callipaeda (Spirurida, Thelaziidae) infection has on the survival of its vector Phortica variegata (Drosophilidae, Steganinae) is scarce. The present study aimed to: (a) assess the rate of infection between experimentally infected (EI) and not experimentally infected (NEI) flies and (b) determine how T. callipaeda infection may affect the survival of P. variegata. In addition, fat composition was evaluated in flies that died during overwintering. Molecular analysis showed that T. callipaeda prevalence in flies that died before experimental infection, plus those from the NEI group, is 0.75% (i.e. 11 out of 1462 individuals). The EI group showed a significantly higher positivity to T. callipaeda (i.e. 51 out of 682 individuals; 7.48%) compared with the NEI group (i.e. 9 out of 750 individuals; 1.2%). Thelazia callipaeda DNA was detected until 147 days after experimental infection. This demonstrates that larvae of this eyeworm may survive in the fly for a significant period of the winter. Fat composition analysis showed that flies produced more unsaturated than saturated fatty acids during diapause, probably because unsaturated fatty acids remain in a liquid state at lower temperatures, providing anti-freeze properties to survive winter. 相似文献
547.
Stephen J. Kaufman Christine M. Parks Joseph Bohn Lia E. Faiman 《Experimental cell research》1980,125(2):333-349
The differentiation of skeletal muscle is characterized by cessation of proliferation and fusion of single myoblasts to form non-replicating multinucleate fibers (myotubes). If termination of proliferation is an obligate requirement for further differentiation, myoblasts defective in this stage of development should fail to fuse or exhibit any further characteristics of myotubes. Furthermore, myoblasts which have lost the ability to control and cease proliferation may represent a transformed, potentially tumorigenic population. Formation of the neoplastic state may therefore be viewed as an alternate path, antithetical to the normal differentiation of skeletal muscle. To test this hypothesis, we isolated 13 clones of non-fusing cells from the myogenic L8 line of rat myoblasts. In contrast to the L8 line, all of the non-fusing clones maintain their proliferative capacity, do not form myotubes, nor elevated creatine kinase activity nor increased myosin, but do develop into tumors when injected into athymic mice. L8 cells do not produce tumors in these mice. Analysis of cell growth and serum requirements, plasminogen activator, hexose transport, adhesiveness, LETS protein and growth in soft agar, indicates that these non-fusing cells are transformed and clearly distinguished from the parent L8 cells. Whereas the L8 line maintains a near diploid complement of chromosomes, all non-fusing clones were polyploid. In addition, 12 of 13 non-fusing clones (but not the L8 cells) express an endogenous type C virus. Although all clones defective in differentiation formed tumors, no single in vitro characteristic was found to be a constant index of this tumorigenic capacity. We conclude that cessation of proliferation is an obligate requirement for skeletal myogenesis, that transformation is an alternative to normal skeletal muscle development and that the phenotype of these transformed cells may be quite varied. 相似文献
548.
Landers Catherine C. Rabeler Christina A. Ferrari Emily K. D’Alessandro Lia R. Kang Diana D. Malisa Jessica Bashir Safia M. Carone Dawn M. 《Chromosoma》2021,130(1):75-90
Chromosoma - Within the pericentric regions of human chromosomes reside large arrays of tandemly repeated satellite sequences. Expression of the human pericentric satellite HSATII is prevented by... 相似文献
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Oxidative stress is a major contributor to kidney injury following ischemia reperfusion. Ferritin, a highly conserved iron-binding protein, is a key protein in the maintenance of cellular iron homeostasis and protection from oxidative stress. Ferritin mitigates oxidant stress by sequestering iron and preventing its participation in reactions that generate reactive oxygen species. Ferritin is composed of two subunit types, ferritin H and ferritin L. Using an in vivo model that enables conditional tissue-specific doxycycline-inducible expression of ferritin H in the mouse kidney, we tested the hypothesis that an increased level of H-rich ferritin is renoprotective in ischemic acute renal failure. Prior to induction of ischemia, doxycycline increased ferritin H in the kidneys of the transgenic mice nearly 6.5-fold. Following reperfusion for 24 hours, induction of neutrophil gelatinous-associated lipocalin (NGAL, a urine marker of renal dysfunction) was reduced in the ferritin H overexpressers compared to controls. Histopathologic examination following ischemia reperfusion revealed that ferritin H overexpression increased intact nuclei in renal tubules, reduced the frequency of tubular profiles with luminal cast materials, and reduced activated caspase-3 in the kidney. In addition, generation of 4-hydroxy 2-nonenal protein adducts, a measurement of oxidant stress, was decreased in ischemia-reperfused kidneys of ferritin H overexpressers. These studies demonstrate that ferritin H can inhibit apoptotic cell death, enhance tubular epithelial viability, and preserve renal function by limiting oxidative stress following ischemia reperfusion injury. 相似文献