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951.
Wenxue Li Aurelie Papilloud Laura Lozano‐Montes Nan Zhao Xueting Ye Xiaozhe Zhang Carmen Sandi Gregor Rainer 《Proteomics》2018,18(7)
Adverse life experiences increase the lifetime risk to several stress‐related psychopathologies, such as anxiety or depressive‐like symptoms following stress in adulthood. However, the neurochemical modulations triggered by stress have not been fully characterized. Neuropeptides play an important role as signaling molecules that contribute to physiological regulation and have been linked to neurological and psychiatric diseases. However, little is known about the influence of stress on neuropeptide regulation in the brain. Here, we have performed an exploratory study of how neuropeptide expression at adulthood is modulated by experiencing a period of multiple stressful experiences. We have targeted hippocampus and prefrontal cortex (PFC) brain areas, which have previously been shown to be modulated by stressors, employing a targeted liquid chromatography‐mass spectrometry (LC‐MS) based approach that permits broad peptide coverage with high sensitivity. We found that in the hippocampus, Met‐enkephalin, Met‐enkephalin‐Arg‐Phe, and Met‐enkephalin‐Arg‐Gly‐Leu were upregulated, while Leu‐enkephalin and Little SAAS were downregulated after stress. In the PFC area, Met‐enkephalin‐Arg‐Phe, Met‐enkephalin‐Arg‐Gly‐Leu, peptide PHI‐27, somatostatin‐28 (AA1‐12), and Little SAAS were all downregulated. This systematic evaluation of neuropeptide alterations in the hippocampus and PFC suggests that stressors impact neuropeptides and that neuropeptide regulation is brain‐area specific. These findings suggest several potential peptide candidates, which warrant further investigations in terms of correlation with depression‐associated behaviors. 相似文献
952.
953.
Yixian Liao Yiming Guo Sumei Li Lei Wang Yongmei Tang Tianmiao Li Weihao Chen Guohua Zhong Gaopeng Song 《Bioorganic & medicinal chemistry letters》2018,28(7):1188-1193
This paper describes our medicinal chemistry efforts on 7-(cyclopentyloxy)-6-methoxy1,2,3,4-tetrahydroisoquinoline scaffold: design, synthesis and biological evaluation using conformational restriction approach and bioisosteric replacement strategy. Biological data revealed that the majority of the synthesized compounds of this series displayed moderate to potent inhibitory activity against PDE4B and strong inhibition of LPS-induced TNFα release. Among them, compound 19 exhibited the strongest inhibition against PDE4B with an IC50 of 0.88?µM and 21 times more potent selectivity toward PDE4B over PDE4D when compared to rolipram. A primary structure-activity relationship study showed that the attachment of CH3O group or CF3O group to the phenyl ring at the para-position was helpful to enhance the inhibitory activity against PDE4B. Moreover, sulfonamide group played a key role in improving the inhibitory activity against PDE4B and subtype selectivity. In addition, the attachment of the additional rigid substituents at the C-3 position of 1,2,3,4-tetrahydroisoquinoline ring was favored to subtype selectivity, which was consistent well with the observed docking simulation. 相似文献
954.
Kenyi Saito-Diaz Hassina Benchabane Ajit Tiwari Ai Tian Bin Li Joshua J. Thompson Annastasia S. Hyde Leah M. Sawyer Jeanne N. Jodoin Eduardo Santos Laura A. Lee Robert J. Coffey R. Daniel Beauchamp Christopher S. Williams Anne K. Kenworthy David J. Robbins Yashi Ahmed Ethan Lee 《Developmental cell》2018,44(5):566-581.e8
955.
Kazutaka Miyamoto Mizuha Akiyama Fumiya Tamura Mari Isomi Hiroyuki Yamakawa Taketaro Sadahiro Naoto Muraoka Hidenori Kojima Sho Haginiwa Shota Kurotsu Hidenori Tani Li Wang Li Qian Makoto Inoue Yoshinori Ide Junko Kurokawa Tsunehisa Yamamoto Tomohisa Seki Masaki Ieda 《Cell Stem Cell》2018,22(1):91-103.e5
956.
CuS Microspheres with Tunable Interlayer Space and Micropore as a High‐Rate and Long‐Life Anode for Sodium‐Ion Batteries 下载免费PDF全文
Yuanhua Xiao Dangcheng Su Xuezhao Wang Shide Wu Liming Zhou Ying Shi Shaoming Fang Hui‐Ming Cheng Feng Li 《Liver Transplantation》2018,8(22)
Layered transition metal sulfides (LTMSs) have tremendous commercial potential in anode materials for sodium‐ion batteries (SIBs) in large‐scale energy storage application. However, it is a great challenge for most LTMS electrodes to have long cycling life and high‐rate capability due to their larger volume expansion and the formation of soluble polysulfide intermediates caused by the conversion reaction. Herein, layered CuS microspheres with tunable interlayer space and pore volumes are reported through a cost‐effective interaction method using a cationic surfactant of cetyltrimethyl ammonium bromide (CTAB). The CuS–CTAB microsphere as an anode for SIBs reveals a high reversible capacity of 684.6 mAh g?1 at 0.1 A g?1, and 312.5 mAh g?1 at 10 A g?1 after 1000 cycles with high capacity retention of 90.6%. The excellent electrochemical performance is attributed to the unique structure of this material, and a high pseudocapacitive contribution ensures its high‐rate performance. Moreover, in situ X‐ray diffraction is applied to investigate their sodium storage mechanism. It is found that the long chain CTAB in the CuS provides buffer space, traps polysulfides, and restrains the further growth of Cu particles during the conversion reaction process that ensure the long cycling stability and high reversibility of the electrode material. 相似文献
957.
Both global change and biological invasions threaten biodiversity worldwide. However, their interactions and related mechanisms are still not well elucidated. To elucidate potential traits contributing to invasiveness and whether ongoing increase in CO2 aggravates invasions, noxious invasive Wedelia trilobata and native Wedelia urticifolia and Wedelia chinensis were compared under ambient and doubled atmospheric CO2 concentrations in terms of growth, biomass allocation, morphology, and physiology. The invader had consistently higher leaf mass fraction (LMF) and specific leaf area than the natives, contributing to a higher leaf area ratio, and therefore to faster growth and invasiveness. The higher LMF of the invader was due to lower root mass fraction and higher fine root percent. On the other hand, the invader allocated a higher fraction of leaf nitrogen (N) to photosynthetic apparatus, which was associated with its higher photosynthetic rate, and resource use efficiency. All these traits collectively contributed to its invasiveness. CO2 enrichment increased growth of all studied species by increasing actual photosynthesis, although it decreased photosynthetic capacities due to decreased leaf and photosynthetic N contents. Responses of the invasive and native plants to elevated CO2 were not significantly different, indicating that the ongoing increase in CO2 may not aggravate biological invasions, inconsistent with the prevailing results in references. Therefore, more comparative studies of related invasive and native plants are needed to elucidate whether CO2 enrichment facilitates invasions. 相似文献
958.
Atsuko Takagi Yasuyuki Ikeda Kunihisa Kobayashi Kazuhiro Kobayashi Yoshihiko Ikeda Junji Kozawa Hideyuki Miyauchi Ming Li Chikako Hashimoto Yasuhiro Hara Satoshi Yamaguchi Akira Suzuki Tatsushi Toda Hironori Nagasaka Ken-ichi Hirano 《Biochemical and biophysical research communications》2018,495(1):646-651
Triglyceride deposit cardiomyovasculopathy (TGCV) is a rare and newly identified disease among patients requiring cardiac transplantation. TGCV is characterized by cardiomyocyte steatosis and triglyceride (TG)-deposit atherosclerosis, resulting from the abnormal intracellular metabolism of TG. TGCV is classified into primary and idiopathic types. Primary TGCV carries ultra-rare genetic mutations in the adipose triglyceride lipase (ATGL), a rate-liming enzyme that hydrolyzes intracellular TG in adipose and non-adipose tissues. Idiopathic TGCV, first identified among autopsied individuals with diabetes mellitus (DM) with severe heart diseases, shows no ATGL mutations and its causes and underlying mechanisms are still unknown. TGCV is difficult to diagnose in daily clinics, thereby demanding feasible diagnostic procedures. We aimed to develop an assay to measure ATGL activity using peripheral leucocytes. Human his6-ATGL was expressed in COS1 cells, purified to homogeneity, and used to raise a polyclonal antibody neutralizing TG-hydrolyzing activity of ATGL. We developed a selective immunoinactivation assay (SIIA) for the quantitation of ATGL activity in cell lysates of leucocytes by the antibody neutralizing ATGL activities. ATGL activity was measured in 13 idiopathic TGCV patients, with two patients with primary TGCV as the negative control. Healthy (non-DM) and DM controls without heart diseases were also subjected. The developed SIIA assay revealed significant reduction in ATGL activity in leucocytes from patients with idiopathic TGCV who did not carry ATGL mutations as compared with non-DM and DM controls. Thus, ATGL in leucocytes may be an important biomarker for the diagnosis of TGCV and our assay may provide insights into pathophysiology and elucidate the underlying mechanism of TGCV and related disorders. 相似文献
959.
Han Wang Qian Zhang BinBin Wang WangJun Wu Julong Wei Pinghua Li Ruihua Huang 《European journal of cell biology》2018,97(4):257-268
Recently, miR-22 was found to be differentially expressed in different skeletal muscle growth period, indicated that it might have function in skeletal muscle myogenesis. In this study, we found that the expression of miR-22 was the most in skeletal muscle and was gradually up-regulated during mouse myoblast cell (C2C12 myoblast cell line) differentiation. Overexpression of miR-22 repressed C2C12 myoblast proliferation and promoted myoblast differentiation into myotubes, whereas inhibition of miR-22 showed the opposite results. During myogenesis, we predicted and verified transforming growth factor beta receptor 1 (TGFBR1), a key receptor of the TGF-β/Smad signaling pathway, was a target gene of miR-22. Then, we found miR-22 could regulate the expression of TGFBR1 and down-regulate the Smad3 signaling pathway. Knockdown of TGFBR1 by siRNA suppressed the proliferation of C2C12 cells but induced its differentiation. Conversely, overexpression of TGFBR1 significantly promoted proliferation but inhibited differentiation of the myoblast. Additionally, when C2C12 cells were treated with different concentrations of transforming growth factor beta 1 (TGF-β1), the level of miR-22 in C2C12 cells was reduced. The TGFBR1 protein level was significantly elevated in C2C12 cells treated with TGF-β1. Moreover, miR-22 was able to inhibit TGF-β1-induced TGFBR1 expression in C2C12 cells. Altogether, we demonstrated that TGF-β1 inhibited miR-22 expression in C2C12 cells and miR-22 regulated C2C12 cell myogenesis by targeting TGFBR1. 相似文献