全文获取类型
收费全文 | 184757篇 |
免费 | 26323篇 |
国内免费 | 13486篇 |
专业分类
224566篇 |
出版年
2024年 | 358篇 |
2023年 | 2027篇 |
2022年 | 4614篇 |
2021年 | 8098篇 |
2020年 | 7002篇 |
2019年 | 9657篇 |
2018年 | 9413篇 |
2017年 | 8248篇 |
2016年 | 10239篇 |
2015年 | 13213篇 |
2014年 | 14777篇 |
2013年 | 15733篇 |
2012年 | 16215篇 |
2011年 | 14725篇 |
2010年 | 11029篇 |
2009年 | 9089篇 |
2008年 | 9214篇 |
2007年 | 8132篇 |
2006年 | 7080篇 |
2005年 | 5857篇 |
2004年 | 5006篇 |
2003年 | 4647篇 |
2002年 | 3885篇 |
2001年 | 3273篇 |
2000年 | 3019篇 |
1999年 | 2797篇 |
1998年 | 1634篇 |
1997年 | 1519篇 |
1996年 | 1498篇 |
1995年 | 1307篇 |
1994年 | 1281篇 |
1993年 | 997篇 |
1992年 | 1364篇 |
1991年 | 1040篇 |
1990年 | 844篇 |
1989年 | 780篇 |
1988年 | 628篇 |
1987年 | 607篇 |
1986年 | 507篇 |
1985年 | 517篇 |
1984年 | 313篇 |
1983年 | 316篇 |
1982年 | 223篇 |
1981年 | 160篇 |
1980年 | 144篇 |
1979年 | 187篇 |
1977年 | 138篇 |
1974年 | 149篇 |
1973年 | 132篇 |
1972年 | 126篇 |
排序方式: 共有10000条查询结果,搜索用时 25 毫秒
1.
2.
3.
4.
We evaluated the cytotoxic and DNA cross-linking (CL) ability of four second generation platinum coordination complexes (TNO-6, JM-89, JM-8 and JM-9) delivered alone or in combination with 1-beta-D-arabinofuranosyl cytosine (ara-C) to human colon cancer cells (LoVo). Cell survival varied markedly as a function of the particular substitution moiety. JM-8 and JM-9 were virtually ineffective, even at concentrations as high as 50 micrograms/ml. At that concentration cis-diamminedichloroplatinum(II) (cis-DDP) killed greater than 99.99% of the cells. JM-82 was slightly more active while TNO-6 was the only derivative with appreciably higher cytotoxic activity due to an abrogation of the shoulder region of the type C survival curve. The highest CL effect was observed for cis-DDP followed closely by TNO-6. Very little CL effects were demonstrated for the other three analogs JM-82, JM-8 and JM-9 when measured 6 h after treatment. The combination of cis-DDP and ara-C augmented 10-fold the cytotoxic activity of cis-DDP alone, an effect accompanied by an almost 2-fold increase in CL; every other analog failed to interact in a potentiating manner (either cytotoxicity, or CL at 6 h) with the antimetabolite. Thus, it appears clear that the associated moieties of the Pt coordination complex play a fundamental role in reducing the interaction of the analogs with DNA (as reflected by the decreased CL and cytotoxic effects produced by each agent alone) and in totally preventing their interaction with ara-C to yield a potentiating lethal effect. 相似文献
5.
J R Vandenheede S D Yang W Merlevede 《Biochemical and biophysical research communications》1983,115(3):871-877
The major active protein phosphatase present in a rabbit skeletal muscle extract is associated with the glycogen particle and migrates in sucrose density gradient centrifugation as a Mr = 70,000 protein and contains modulator activity. Addition of extra modulator protein causes a time- and concentration-dependent conversion of the enzyme to an inactive FA-ATP, Mg-dependent form. The intrinsic modulator in the active phosphatase is destroyed by limited proteolysis without an appreciable change in the phosphatase activity. The proteolyzed active enzyme has a lower molecular weight (Mr = 40,000) and it reassociates with the modulator producing a FA-ATP, Mg-dependent enzyme form (Mr = 60,000). The modulator protein is used stoichiometrically in the activation of the ATP, Mg-dependent phosphatase. This is in agreement with the presence of one unit of modulator activity per unit of native spontaneously active phosphatase. 相似文献
6.
Characterization of phosphotyrosyl-protein phosphatase activity associated with calcineurin 总被引:3,自引:0,他引:3
J Chernoff M A Sells H C Li 《Biochemical and biophysical research communications》1984,121(1):141-148
Calcineurin purified from bovine brain is shown to possess phosphotyrosyl -protein phosphatase activity towards proteins phosphorylated by the epidermal growth factor receptor/kinase. The phosphatase activity is augmented by Ca2+/calmodulin or divalent cation (Ni2+ greater than Mn2+ greater than Mg2+ greater than Co2+). In the simultaneous presence of all three effectors, the enzymatic activity is synergistically increased. Ca2+/calmodulin activates the Mg2+-supported activity by decreasing the Km value for phosphotyrosyl -casein from 2.2 to 0.6 microM, and increasing the Vmax from 0.4 to 4.6 nmol/min/mg. These results represent the first demonstration that calcineurin can dephosphorylate phosphotyrosyl -proteins and suggest a novel mechanism of activation of this enzyme. 相似文献
7.
E Giannazzo F Licata G Li Volsi G Mio F Santangelo M Scivoli 《Bollettino della Società italiana di biologia sperimentale》1992,68(11):677-683
A computer-assisted analysis of the spatial distribution of neurons having homogeneous characteristics is described in this paper. The camera lucida drawings of sections of a brain nucleus and the points representing the neurons labeled on the basis of a specific behavior of discharge rates were digitized on a personal computer Amiga 2000 or IBM compatible. Our software provided: a) the computerized, stereotaxically oriented reconstruction of the stored sections and of the plotted neurons; b) the identification within each section of the mass center (MC) of the units sharing a given behavior and of the area where the density of such neurons was maximal (MDA). The routine was tested on the spatial distribution of neuronal responses to serotonin in the lateral vestibular nucleus. 相似文献
8.
Determination of oxalyl thiolesters, N-oxalylcysteine and N-oxalylcysteamine in biological materials
A convenient method is described for the quantitative analysis of oxalyl thiolesters (OTEs), a newly discovered class of mammalian metabolites, in biological samples. By this particular technique the total concentration of all OTEs in the sample is determined. The method involves first reacting the biological material with cysteamine (2-aminoethanethiol) or cysteine under conditions that convert OTEs quantitatively to N-oxalylcysteamine (or N-oxalylcysteine), followed by reaction with monobromobimane to give a highly fluorescent derivative that is analyzed by reversed-phase ion-pair chromatography, with tetrabutylammonium ion as the counterion and N-(2-mercaptopropionyl)glycine as an internal standard. The method is capable of detecting as little as 0.6 pmol of the bimane derivative of the N-oxalyl compound in a single HPLC injection. The application of this method has led to the discovery that not only OTEs but also N-oxalylcysteine and N-oxalylcysteamine are normal mammalian metabolites. In various rat tissues the OTE concentration ranges up to 65 nmol/g (wet wt), the N-oxalylcysteine concentration is approximately 10 nmol/g, and the N-oxalylcysteamine concentration is 0-3 nmol/g. 相似文献
9.
10.