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341.
Marcelo E. Oliva Isabel M. Valdivia Leyla Cárdenas Gabriela Muñoz Ruben Escribano Mario George-Nascimento 《Parasitology international》2018,67(2):159-169
The most studied digenean of marine organisms in Chile is by far Proctoeces humboldti, a parasite of the intestine of the clingfish Sicyases sanguineus and gonad of the keyhole limpet Fissurella spp. (progenetic metacercariae). The mussel Perumytilus purpuratus has been suggested as the first intermediate host for this digenean. In a study examining the parasites of S. sanguineus from central Chile, we found specimens of Proctoeces showing significant morphological differences with P. humboldti. To assist in the resolution of the taxonomic identification of these specimens, as well sporocysts obtained from the mussel P. purpuratus from central and northern Chile, phylogenetic studies using DNA sequences from the SSU rRNA, as well the LSU rRNA and Cox 1 gene were performed. Results showed that the clingfish S. sanguineus is a host for two species of Proctoeces (P. humboldti and P. syciases n. sp.) along the northern and central Chilean coast, without geographic separation; the mussel P. purpuratus is the first intermediate host for P. syciases n. sp. but not for P. humboldti in central and northern Chile. Fissurellids (Archaeogastropoda) along the Chilean coast harbor only progenetic stages of P. humboldti, but there is no evidence of progenesis for P. syciases. The reinstatement of Proctoeces humboldti is strongly suggested. 相似文献
342.
Zoleykha Asadi Mojtaba Fathi Elham Rismani Zahra Bigdelou Behrooz Johari 《Cell biology international》2021,45(5):1001-1014
343.
Saeed Khalili Mohamad Javad Rasaee Taravat Bamdad Maysam Mard-Soltani Majid Asadi Ghalehni Abolfazl Jahangiri Mohammad Hassan Pouriayevali Mohammad Reza Aghasadeghi Fatemeh Malaei 《Molecular biotechnology》2018,60(11):833-842
Nucleic acid immunization has recently exhibited a great promise for immunotherapy of various diseases. However, it is now clear that powerful strategies are imminently needed to improve their efficiency. In this regard, whole bacteriophage particles have been described as efficient DNA vaccine delivery vehicles, capable of circumventing the limitations of naked DNA immunization. Moreover, phage particles could be engineered to display specific peptides on their surfaces. Given these inherent characteristics of phages, we have designed a novel hybrid phage-DNA immunization vector using both M13 and pAAV plasmid elements. Following the construction and in vitro confirmation of the designed vectors, they were used for comparative mice immunization, carrying the same DNA sequence. The results indicated the efficacy of the designed hybrid phage particles, to elicit higher humoral immunity, in comparison to conventional DNA-immunization vectors (pCI). In light of these findings, it could be concluded that using adeno-associated virus (AAV) expression cassette along with displaying TAT peptide on the surface of the phage particle could be deemed as an appealing strategy to enhance the DNA-immunization and vaccination efficacy. 相似文献
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345.
The impact of alien species on native organisms is a cause for concern worldwide, with biological invasions commonplace today.
Suppression efforts targeting many invasive species have included introductions of biological control agents. The numerous
releases of biological control agents in the Hawaiian archipelago have resulted in considerable concern for non-target impacts,
due to high levels of non-target parasitism observed to occur in some cases. This study investigated the impact of introduced
Hymenoptera parasitoids on a Hawaiian moth. The endemic Hawaiian moth Udea stellata (Butler) has seven alien parasitoids associated with it, two purposely introduced, three adventive, and two of uncertain
origin. The objective of this study was to determine the relative contribution of the seven parasitoid species to the population
dynamics of U. stellata by constructing partial life tables. Marginal attack rates and associated k-values were calculated to allow comparison of mortality factors between experimental sites. Sentinel larvae were deployed
on potted host plants and left in the field for 3-day intervals in open and exclusion treatments. The factors that contributed
to total mortality in the open treatment were: disappearance (42.1%), death due to unknown reasons during rearing (16.5%)
and parasitism (4.9%). The open treatment incurred significantly higher larval disappearance compared to the exclusion treatment
(7.8%), which suggests that in large part disappearance is the result of predation. Adventive parasitoids inflicted greater
total larval mortality attributable to parasitism (97.0%) than purposely introduced species (3.0%). 相似文献
346.
Genetic variation assessment of acid lime accessions collected from south of Iran using SSR and ISSR molecular markers 总被引:1,自引:0,他引:1
Ata Allah Sharafi Asad Asadi Abkenar Ali Sharafi Mohammad Masaeli 《Physiology and Molecular Biology of Plants》2016,22(1):87-95
Iran has a long history of acid lime cultivation and propagation. In this study, genetic variation in 28 acid lime accessions from five regions of south of Iran, and their relatedness with other 19 citrus cultivars were analyzed using Simple Sequence Repeat (SSR) and Inter-Simple Sequence Repeat (ISSR) molecular markers. Nine primers for SSR and nine ISSR primers were used for allele scoring. In total, 49 SSR and 131 ISSR polymorphic alleles were detected. Cluster analysis of SSR and ISSR data showed that most of the acid lime accessions (19 genotypes) have hybrid origin and genetically distance with nucellar of Mexican lime (9 genotypes). As nucellar of Mexican lime are susceptible to phytoplasma, these acid lime genotypes can be used to evaluate their tolerance against biotic constricts like lime “witches’ broom disease”. 相似文献