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51.
J Owens R M Lewis A Cantor B C Furie B Furie 《The Journal of biological chemistry》1984,259(22):13800-13805
A monoclonal antibody JO1 X 1 was prepared against human abnormal prothrombin using the hybridoma technique. The clone secreting this antibody was selected on the basis of the ability of this antibody to bind to abnormal prothrombin, but not to prothrombin, in the presence of calcium ions. The antibodies were purified by affinity chromatography in EDTA on columns of prothrombin-Sepharose. Bound antibodies were eluted with 15 mM CaCl2. The kinetics of dissociation of antibody from the antibody-prothrombin complex with the addition of calcium ions fit a first-order kinetic model. Increasing CaCl2 concentration increased the rate of antibody-prothrombin dissociation. Ca(II) and Mn(II) inhibited antibody-prothrombin interaction; half-maximal binding was observed at 0.9 and 4 mM, respectively. Mg(II) had little effect on antibody-antigen interaction. The JO1 X 1 antibody bound fragment 1, fragment (1-39), abnormal prothrombin, and prothrombin equivalently in the presence of EDTA, but did not bind to des(1-44)prothrombin in the presence of EDTA or prothrombin in the presence of CaCl2. These results indicate that the monoclonal antibody JO1 X 1 is conformation specific for the calcium-free conformer of prothrombin and directed against an antigenic determinant near the NH2 terminus of prothrombin expressed in the 1-39 region of the protein. This analysis provides confirmation of the presence of a metal-free conformer of prothrombin. 相似文献
52.
Bryan B. Fuller 《In vitro cellular & developmental biology. Plant》1987,23(9):633-640
Summary Tyrosinase activity increased in Cloudman S-91 mouse melanoma cell homogenates incubated at 37°C for a minimum of 8 h. Enzyme
activity continued to increase for 48h at which time the maximal level of activation was observed. Activation did not occur
at 4°C and did not occur in the cytosol fraction of the cell, suggesting that the response was localized to melanosomes. The
activated enzyme was resistant to solubilization with the nonionic detergent, Triton X-100, and preparation of homogenates
in this detergent did not inhibit the temperature-dependent activation of the melanosomal fraction of the cell. The activation
process increased the V
Max
of tyrosinase 10-fold and lowered the K
M
by a factor of 2 as determined by the tyrosine hydroxylase assay. The increase in tyrosinase activity was detectable by three
assay methods: tyrosine hydroxylation, melanin synthesis, and by tyrosine decarboxylation. The formation of melanin, however,
was found to be 1/20 that of either tyrosine hydroxylation or decarboxylation, a finding which suggests that the melanin pathway
may be blocked at 5,6-dihydroxyindole. The “self-activation” response could not be mimicked by incubating cell homogenates
with cyclic AMP-dependent protein kinase. Activated tyrosinase could be inhibited by the addition of fresh cell extracts,
a finding which suggests that tyrosinase inhibitors may be present in these cells.
This investigation was supported by Public Health Service grants CA41425 and CA30393 awarded by the National Cancer Institute,
Bethesda, MD and by a research grant from the Proctor and Gamble Company. 相似文献
53.
R. J. Clarke D. Lincoln Lewis J. Alexander Williams 《BMJ (Clinical research ed.)》1972,2(5810):369-371
In a series of 68 patients with gastric ulcer who were treated by vagotomy and pyloroplasty there were no operative deaths, though one patient who received massive transfusions died 14 weeks later from hepatic necrosis probably associated with serum hepatitis. Four patients developed recurrent peptic ulceration requiring further surgical treatment. The clinical condition of all but three of the remainder was satisfactory after an average follow-up period of three years and two months. Of the 14 patients who presented with an acutely bleeding gastric ulcer, one subsequently required gastrectomy for continued bleeding; in all the others the immediate and long-term results were satisfactory. Of 21 patients with a “high” ulcer and 29 with combined gastric and duodenal ulceration one (4·8%) and three (10%) respectively suffered recurrences. We conclude that vagotomy and pyloroplasty is a satisfactory form of treatment for a high or bleeding gastric ulcer, but that for all other gastric ulcers some form of gastric resection is preferable. 相似文献
54.
55.
D. maximum is a dominant species of outer reef flats in the Red Sea, reaching densities of about 22/m2 and biomass of 15.8 g dry tissue/m2 . A few individuals attached to loose rocks are found inside the breaker zone but they may have been dislodged by heavy seas from the outer reef flat. D. maximum feeds from a mucus net which is spread by wave action over the substratum. Hauling the net occurs at approximately 13 minute intervals throughout the 24 hours and lasts about two minutes. Neighbours with overlapping nets stimulate each other to haul and reduce feeding efficiency. The net is grasped by a pair of lateral jaws, tugged free of the substratum by rotation of the body and ingested by a zipper-like action of the lateral and marginal radula teeth. The robust, central and lateral teeth become worn, possibly while channelling out the substratum to accommodate new shell. Defaecation occurs about 2.4 times an hour, amounting to 10450 kcal/m2 /y. Females may brood simultaneously at least 11 egg capsules at various stages of development, which are suspended by stalks from the roof of the shell and pass through a dorsal slit in the mantle. Each capsule contains–500 embryos which develop into larvae with simple, coiled shells 0.33 mm in diameter. There is no planktonic phase. Adult shells amount to 2.5 kg/m2 on the outer reef flat, while dead shells are often occupied by blennies. Although D. maximum is not a specialized filter feeder, the highly developed ciliary mechanisms suggest that filtering may be an auxiliary feeding method. 相似文献
56.
This paper describes the isolation and amino acid analysis of un-cross-linked elastin obtained by neutral salt extraction from the ligamentum nuchae of a calf fed from birth to 9 months on a diet low in copper. 相似文献
57.
58.
59.
Synergistic effects of metabolically related amino acids on the growth of a multicellular plant 总被引:2,自引:2,他引:0 下载免费PDF全文
The effects of several amino acids related to the metabolism of aspartic acid on the growth and development of gemmalings of the liverwort Marchantia polymorpha were investigated under axenic conditions. Lysine and theonine synergistically inhibit the growth of these plants and cause a loss of normal pigmentation at concentrations as low as 1 μm. These effects are highly specific for this pair of amino acids, are partially reversible upon removal of the effectors, and can be prevented by low concentrations of methionine or its metabolic precursor, homoserine. Alterations in the growth and development of gemmalings in the presence of natural amino acids are discussed in relation to metabolic regulatory mechanisms which have been well established in microorganisms. 相似文献
60.