全文获取类型
收费全文 | 1486篇 |
免费 | 144篇 |
出版年
2021年 | 31篇 |
2020年 | 17篇 |
2019年 | 27篇 |
2018年 | 27篇 |
2017年 | 17篇 |
2016年 | 30篇 |
2015年 | 59篇 |
2014年 | 54篇 |
2013年 | 59篇 |
2012年 | 92篇 |
2011年 | 95篇 |
2010年 | 54篇 |
2009年 | 50篇 |
2008年 | 52篇 |
2007年 | 59篇 |
2006年 | 58篇 |
2005年 | 42篇 |
2004年 | 47篇 |
2003年 | 42篇 |
2002年 | 46篇 |
2001年 | 23篇 |
2000年 | 25篇 |
1999年 | 25篇 |
1998年 | 11篇 |
1997年 | 11篇 |
1995年 | 16篇 |
1994年 | 11篇 |
1993年 | 12篇 |
1992年 | 28篇 |
1991年 | 26篇 |
1990年 | 29篇 |
1989年 | 38篇 |
1988年 | 35篇 |
1987年 | 25篇 |
1986年 | 14篇 |
1985年 | 13篇 |
1983年 | 14篇 |
1982年 | 11篇 |
1981年 | 10篇 |
1980年 | 17篇 |
1979年 | 17篇 |
1978年 | 11篇 |
1977年 | 13篇 |
1976年 | 12篇 |
1975年 | 21篇 |
1974年 | 13篇 |
1973年 | 16篇 |
1972年 | 10篇 |
1970年 | 13篇 |
1968年 | 11篇 |
排序方式: 共有1630条查询结果,搜索用时 15 毫秒
61.
Twenty-seven patients with 32 acute exacerbations of Crohn''s disease were treated for four weeks with an elemental diet. At the end of treatment 29 of the exacerbations had remitted both clinically and biochemically. After six months six patients had relapsed. These findings suggest that the elemental diet is effective in treating acute Crohn''s disease, but the reasons are not clear. The diet may be effective because it provides nutritional support, is hypoallergenic, acts as a medical bypass from the affected area, or alters bowel flora. The patient''s general wellbeing is improved by the supply of adequate energy and essential foodstuffs in a form easily available without further digestion and given in a safe, simple, non-toxic way. 相似文献
62.
During the course of operating high-rate algae ponds (HRAP) for wastewater treatment and protein production, changes were found in the two main algae species. The observed changes were interpreted to be a reflection of the operation regime and loading combined with environmental conditions. To verify that these changes were phenotypic and not genetic, experiments were conducted on Scenedesmus dimorphus growing in miniponds (110 L) as well as in the laboratory. The results showed that the changes in S. dimorphus were external and due to the changes in the loading and operating conditions of the ponds adjusted to changing environmental conditions. It was found that wastewater treatment efficiency and algal yield are also correlated with the S. dimorphus type. 相似文献
63.
64.
Regulatory Properties of the ADP-Glucose Pyrophosphorylase of the Blue-Green Bacterium Synechococcus 6301 下载免费PDF全文
ADP-glucose was found to be the primary sugar nucleotide used for glycogen synthesis by Synechococcus 6301. ADP-glucose pyrophosphorylase was partially purified 12-fold from this blue-green bacterium. The enzyme was activated 8- to 25-fold by glycerate 3-phosphate. Fructose 6-phosphate, fructose 1,6-bisphosphate, 5'-adenylate, and adenosine diphosphate activated the enzyme, but less than glycerate 3-phosphate. The enzyme was inhibited by inorganic phosphate. The I(0.5) of phosphate was 0.072 mm, and in the presence of 2 mm glycerate 3-phosphate, increased to 1.8 mm. The substrate saturation curves for glucose 1-phosphate and ATP were hyperbolic in both the presence and absence of glycerate 3-phosphate or phosphate. The saturation curve for MgCl(2) was sigmoidal; 2 mm glycerate 3-phosphate decreased the sigmoidicity from a Hill slope n value of 5.6 to 2.8, and increased the MgCl(2) optimum from 3 mm to 6 to 7 mm. 相似文献
65.
The hyphal walls of three mycobionts, isolated from the lichens Xanthoria parietina, Tornabenia intricata and Sarcogyne sp. were investigated by two techniques: microautoradiography of fungal colonies exposed to radioactive carbohydrate precursors; and binding, in vivo, of fluorescein conjugated lectins to hyphal walls of such colonies.N-[3H] acetylglucosamine was readily incorporated into tips, young hyphal walls and septa of the three mycobionts and the free-living fungus Trichoderma viride, but not into Phytophthora citrophthora, indicating that chitin is a major component of the mycobionts' hyphal walls. All three mycobionts, but neither of the free-living fungi, incorporated [3H] mannose and [3H] mannitol into their hyphal walls.Fluorescein-conjugated wheat germ agglutinin was bound to the hyphal walls of the three mycobionts and T. viride, but not to the walls of P. citrophthora; the binding pattern was similar to the grain pattern obtained in autoradiographs after short N-[3H] acetylglucosamine labelling. As wheat germ agglutinin binds specifically to chitin oligomers, the lectin binding tests further confirmed that chitin is a mycobiont hyphal wall component.Binding characteristics of several fluorescein-conjugated lectins to the three mycobionts indicated that this technique can yield useful information concerning the chemical composition of hyphal wall surfaces.List of abbreviations FITC
fluorescein isothiocyanate
- WGA
wheat germ agglutinin
- TCA
trichloroacetic acid
- PNA
peanut agglutinin
- LA
lotus agglutinin
- Glc NAc
N-acetylglucosamine
- ConA
concanavalin A
- SBA
soybean agglutinin
- WBA
waxbean agglutinin
Part of an M.Sc. thesis submitted by A. Braun to the Department of Botany, Tel Aviv University. 相似文献
66.
The carrier-mediated transport of GABA in rat brain synaptosomes was strongly and permanently inhibited byl-2,4-diaminobutyric acid (DAB). In order to discriminate between carrier-mediated and non-carrier-mediated release of [3H]GABA, synaptosomes prelabeled with 0.5 M [3H]GABA in the presence of 100 M DAB, or with 0.2 M [3H]GABA without DAB, were superfused in conditions stimulating the release of [3H]GABA. Only the release elicited by unlabeled GABA or DAB (by homo- and heteroexchange, respectively) was strongly inhibited in DAB-pretreated synaptosomes. The spontaneous release and the release induced by 56 mM KCl in the presence of CaCl2, by the ionophore A23187, by ouabain, by lack of K+, or by purified black widow spider toxin were unaffected or only barely decreased in DAB-treated synaptosomes, and therefore do not seem to be mediated by the DAB-blocked GABA carrier. 相似文献
67.
The effects of the ionophore A23187 and of ouabain on the release of [3H]GABA and [3H]norepinephrine were studied in superfused rat brain synaptosomes. Each of the two drugs moderately stimulated the spontaneous release of [3H]GABA, but greatly potentiated the release of [3H]GABA induced by unlabeled GABA. In contrast, the ionophore and norepinephrine showed an additive, but not a supraadditive, releasing effect on synaptosomal [3H]norepinephrine. Ouabain modestly and transiently potentiated the norepinephrine-induced [3H]norepinephrine release, which, however, was inhibited by the drug after a few minutes. It is suggested that in the new intrasynaptosomal ionic conditions determined by the two drugs, the stoichiometry of the basal homoexchange of GABA is changed in a direction favoring net outward transport. 相似文献
68.
A S Levi 《Archives of biochemistry and biophysics》1975,168(1):115-121
Lactate dehydrogenase enzyme was immobilized by binding to a cyanogen bromideactivated Sepharose 4B-200 in 0.1 m phosphate buffer, pH 8.5. The immobilized enzyme was found to have lower Km values for its substrates. Km values for pyruvate and lactate were 8 × 10 ?5m and 4 × 10?3m, respectively, an order of magnitude less than the value for the native (free) enzyme. Chicken heart (H4) lactate dehydrogenase was found to lose nearly all its substrate inhibition characteristics as a result of immobilization. The covalently bound muscle-type subunits of lactate dehydrogenase showed more favorable interaction with the muscle type than with the heart type subunits. An increase in thermal and acid stability of the dogfish muscle (M4) lactate dehydrogenase as well as a decrease in the percentage of inhibition of enzyme activity by rabbit antisera and in the complement fixation was observed as a result of immobilization. The changes in the properties of the enzyme as a result of immobilization may be attributable to hindrance produced by the insoluble matrix as well as conformational changes in the enzyme molecules. 相似文献
69.
70.