全文获取类型
收费全文 | 2516篇 |
免费 | 208篇 |
国内免费 | 5篇 |
专业分类
2729篇 |
出版年
2022年 | 33篇 |
2021年 | 49篇 |
2020年 | 18篇 |
2019年 | 19篇 |
2018年 | 24篇 |
2017年 | 21篇 |
2016年 | 56篇 |
2015年 | 107篇 |
2014年 | 125篇 |
2013年 | 120篇 |
2012年 | 184篇 |
2011年 | 181篇 |
2010年 | 106篇 |
2009年 | 86篇 |
2008年 | 124篇 |
2007年 | 109篇 |
2006年 | 122篇 |
2005年 | 96篇 |
2004年 | 117篇 |
2003年 | 102篇 |
2002年 | 87篇 |
2001年 | 78篇 |
2000年 | 84篇 |
1999年 | 58篇 |
1998年 | 32篇 |
1997年 | 25篇 |
1996年 | 21篇 |
1995年 | 13篇 |
1994年 | 23篇 |
1993年 | 9篇 |
1992年 | 31篇 |
1991年 | 44篇 |
1990年 | 22篇 |
1989年 | 36篇 |
1988年 | 27篇 |
1987年 | 47篇 |
1986年 | 28篇 |
1985年 | 36篇 |
1984年 | 28篇 |
1983年 | 20篇 |
1982年 | 17篇 |
1981年 | 12篇 |
1980年 | 7篇 |
1979年 | 23篇 |
1978年 | 16篇 |
1977年 | 21篇 |
1976年 | 9篇 |
1975年 | 14篇 |
1974年 | 17篇 |
1973年 | 9篇 |
排序方式: 共有2729条查询结果,搜索用时 15 毫秒
11.
Modification of susceptibility to Klebsiella pneumoniae during murine cytomegalovirus infection 总被引:1,自引:0,他引:1
The effect of murine cytomegalovirus (MCMV) infection on susceptibility to bacterial infection was studied in mice by a combination of intraperitoneal (ip) inoculation of a sublethal dose of MCMV with subsequent ip challenge of 2 X 10(3) cfu of a strain of Klebsiella pneumoniae (KP). When given alone, KP produced a mortality of 30-40%. Mortality was increased when KP was given 1 to 7 days after MCMV injection with the peak increase at the 4th to 5th day when 100% mortality occurred. Virus levels in various organs of mice infected with MCMV alone, or superinfected with KP did not differ. Bacterial counts on the other hand, showed that increased mortality in mixed MCMV and KP infected mice was due to an uncontrolled growth of bacteria at the site of primary lodgment, i.e., the peritoneum, and severe systemic infection. Neutrophil response to growth of KP during the first 3 days of bacterial infection was defective in MCMV infected mice. While the initial clearance of KP from the blood was more efficient in MCMV infected mice, probably due to activated reticuloendothelial function, it did not protect the mice against KP infection. Using the in vivo model, it was shown that poor neutrophil response and possibly other defective neutrophil functions were responsible for increased mortality to KP infection in MCMV infected mice. 相似文献
12.
The isolation of cod muscle tropomyosin by heat treatment 总被引:1,自引:0,他引:1
13.
14.
Spider toxins selectively block calcium currents in Drosophila 总被引:6,自引:0,他引:6
Toxins from spider venom, originally purified for their ability to block synaptic transmission in Drosophila, are potent and specific blockers of Ca2+ currents measured in cultured embryonic Drosophila neurons using the whole-cell, patch-clamp technique. Differential actions of toxins from two species of spiders indicate that different types of Drosophila neuronal Ca2+ currents can be pharmacologically distinguished. Hololena toxin preferentially blocks a non-inactivating component of the current, whereas Plectreurys toxin blocks both inactivating and non-inactivating components. These results suggest that block of a non-inactivating Ca2+ current is sufficient to block neurotransmitter release at Drosophila neuromuscular junction. 相似文献
15.
C A Brown K Neote A Leung R A Gravel D J Mahuran 《The Journal of biological chemistry》1989,264(36):21705-21710
Lysosomal beta-hexosaminidase (beta-N-acetylhexosaminidase, EC 3.2.1.52) occurs in two major isozyme forms, hexosaminidase A (alpha beta) and hexosaminidase B (beta beta). Although dimer formation is required for enzymatic activity, both subunits contain active sites which share many common substrates. However, the alpha subunit alone confers on hexosaminidase A the specificity for negatively charged substrates, e.g. GM2 ganglioside. Recently, a point mutation, producing a single amino acid substitution in the alpha subunit (Arg178-His), has been found to be associated with the B1 variant phenotype of Tay-Sachs disease (Ohno, K., and Suzuki, K. (1988) J. Neurochem. 50, 316-318). This variant is characterized by normal levels of hexosaminidase A as measured by a common artificial substrate, but an absence of activity toward alpha subunit-specific substrates. However, because of the presence of an active beta subunit in the mutant hexosaminidase A, it has not been possible to determine whether the affected alpha subunit has undergone a change in substrate specificity or become totally inactive. In order to define the full effect of the B1 mutation we have taken advantage of the common evolutionary origin of the genes coding for the alpha and beta subunits. Since the B1 mutation occurs in a region of extended identity between the two subunits, we have duplicated the Arg178-His mutation in a cDNA coding for the human beta subunit (Arg211-His). By expression of the mutant construct in monkey COS cells we have been able to examine the effect of this mutation on beta subunits which are capable of forming stable, active homodimers, an experiment that could not readily be accomplished with heterodimeric hexosaminidase A. Our data show that beta homodimers containing the Arg211-His substitution are formed and are transported into the lysosome in a manner identical to that of normal pro-hexosaminidase B. However, the mutant homodimers are processed at a slower rate and are less stable in the lysozyme. Their most striking feature was a total lack of normal hexosaminidase B activity. We conclude that while the effect of the Arg178-His substitution is not strictly limited to the active site, the severe B1 phenotype results from a totally inactive alpha-subunit in hexosaminidase A. 相似文献
16.
Transforming growth factor-alpha in the mammalian brain. Immunohistochemical detection in neurons and characterization of its mRNA 总被引:5,自引:0,他引:5
J E Kudlow A W Leung M S Kobrin A J Paterson S L Asa 《The Journal of biological chemistry》1989,264(7):3880-3883
In this communication, we demonstrate that adult mammalian brain neurons express transforming growth factor-alpha (TGF-alpha). We used the anti-TGF-alpha monoclonal antibody, MF9, to immunohistochemically localize TGF-alpha in human and rat brain. We found specific immunoreactivity in neurons throughout the brain which was not a result of cross-reactivity of MF9 with the neuropeptide, synenkephalin. Northern blot analysis of bovine and rat brain RNA using human and rat TGF-alpha cDNA probes, respectively, revealed a single 4.8-kilobase pair mRNA with approximately equal abundance in the bovine brainstem, cerebellum, hypothalamus, and cerebral cortex. Fetal rat brain had about 2-fold more TGF-alpha mRNA than did adult rat. The brain TGF-alpha cDNA was cloned from a human neonatal brainstem library. Four identical clones were isolated after screening 10(6) recombinant lambda gt11 phage. The sequence of the 894-base pair cDNA was virtually identical with the cDNA isolated from a human renal cell carcinoma. A single alanine codon was deleted in the brain cDNA at an exon-exon junction. The alanine deletion is within the amino-terminal region of the TGF-alpha precursor that is thought to be removed by proteolytic processing of the precursor to the mature growth factor. These studies indicate that the normal mammalian brain neurons express TGF-alpha. 相似文献
17.
18.
19.
20.
Differential regulation of vascular cell adhesion molecule 1 gene expression by specific NF-kappa B subunits in endothelial and epithelial cells. 总被引:21,自引:5,他引:16 下载免费PDF全文
H B Shu A B Agranoff E G Nabel K Leung C S Duckett A S Neish T Collins G J Nabel 《Molecular and cellular biology》1993,13(10):6283-6289