全文获取类型
收费全文 | 412篇 |
免费 | 43篇 |
出版年
2022年 | 3篇 |
2021年 | 6篇 |
2020年 | 5篇 |
2019年 | 10篇 |
2018年 | 17篇 |
2017年 | 5篇 |
2016年 | 11篇 |
2015年 | 19篇 |
2014年 | 18篇 |
2013年 | 21篇 |
2012年 | 16篇 |
2011年 | 22篇 |
2010年 | 14篇 |
2009年 | 17篇 |
2008年 | 17篇 |
2007年 | 15篇 |
2006年 | 16篇 |
2005年 | 13篇 |
2004年 | 10篇 |
2003年 | 18篇 |
2002年 | 13篇 |
2001年 | 6篇 |
2000年 | 10篇 |
1999年 | 6篇 |
1998年 | 11篇 |
1997年 | 9篇 |
1996年 | 5篇 |
1995年 | 3篇 |
1994年 | 3篇 |
1992年 | 13篇 |
1991年 | 4篇 |
1989年 | 3篇 |
1988年 | 3篇 |
1987年 | 6篇 |
1985年 | 6篇 |
1984年 | 8篇 |
1983年 | 4篇 |
1982年 | 6篇 |
1981年 | 9篇 |
1980年 | 3篇 |
1979年 | 8篇 |
1978年 | 6篇 |
1977年 | 5篇 |
1975年 | 5篇 |
1974年 | 4篇 |
1972年 | 4篇 |
1971年 | 3篇 |
1969年 | 2篇 |
1968年 | 3篇 |
1954年 | 2篇 |
排序方式: 共有455条查询结果,搜索用时 343 毫秒
81.
Florence Paynel Christelle Leroux Ogier Surcouf Annick Schaumann Jérôme Pelloux Azeddine Driouich Jean Claude Mollet Patrice Lerouge Arnaud Lehner Alain Mareck 《Plant Growth Regulation》2014,74(3):285-297
Pectins are major components of primary cell wall that play a crucial role in plant development. After biosynthesis, pectins are secreted in the cell wall by Golgi-derived vesicles under a highly methylesterified form and are de-methylesterified by pectin methylesterases (PME). It is hypothesized that PME might be regulated by pectin methylesterase inhibitor (PMEI). In this paper, we show by isoelectric focalisation and subsequent zymogram that kiwi PMEI was able to inhibit Arabidopsis PME activity by forming a complex. The complexes were stable under a wide range of ionic strength and pH. Moreover, PMEI might be able to form a complex with basic PMEs including three PMEs strongly expressed in root and four PMEs expressed in pollen grains. Finally, exogenous treatment with kiwi PMEI was able to reduce the activity of cell wall resident PMEs with persistent effects such as an increase of the root growth and a dramatic effect on pollen tube stability. 相似文献
82.
Laure Frésard Sophie Leroux Bertrand Servin David Gourichon Patrice Dehais Magali San Cristobal Nathalie Marsaud Florence Vignoles Bertrand Bed'hom Jean-Luc Coville Farhad Hormozdiari Catherine Beaumont Tatiana Zerjal Alain Vignal Mireille Morisson Sandrine Lagarrigue Frédérique Pitel 《Nucleic acids research》2014,42(6):3768-3782
83.
R. Gruwez P. De Frenne A. De Schrijver O. Leroux P. Vangansbeke K. Verheyen 《Annals of botany》2014,113(3):489-500
Background and Aims
Environmental change is increasingly impacting ecosystems worldwide. However, our knowledge about the interacting effects of various drivers of global change on sexual reproduction of plants, one of their key mechanisms to cope with change, is limited. This study examines populations of poorly regenerating and threatened common juniper (Juniperus communis) to determine the influence of four drivers of global change (rising temperatures, nitrogen deposition, potentially acidifying deposition and altering precipitation patterns) on two key developmental phases during sexual reproduction, gametogenesis and fertilization (seed phase two, SP2) and embryo development (seed phase three, SP3), and on the ripening time of seeds.Methods
In 42 populations throughout the distribution range of common juniper in Europe, 11 943 seeds of two developmental phases were sampled. Seed viability was determined using seed dissection and related to accumulated temperature (expressed as growing degree-days), nitrogen and potentially acidifying deposition (nitrogen plus sulfur), and precipitation data.Key Results
Precipitation had no influence on the viability of the seeds or on the ripening time. Increasing temperatures had a negative impact on the viability of SP2 and SP3 seeds and decreased the ripening time. Potentially acidifying depositions negatively influenced SP3 seed viability, while enhanced nitrogen deposition led to lower ripening times.Conclusions
Higher temperatures and atmospheric deposition affected SP3 seeds more than SP2 seeds. However, this is possibly a delayed effect as juniper seeds develop practically independently, due to the absence of vascular communication with the parent plant from shortly after fertilization. It is proposed that the failure of natural regeneration in many European juniper populations might be attributed to climate warming as well as enhanced atmospheric deposition of nitrogen and sulfur. 相似文献84.
Anna Pielach Olivier Leroux David S. Domozych J. Paul Knox Zo? A. Popper 《Annals of botany》2014,114(6):1359-1373
Background and Aims
Parasitic plants obtain nutrients from their hosts through organs called haustoria. The hyaline body is a specialized parenchymatous tissue occupying the central parts of haustoria in many Orobanchaceae species. The structure and functions of hyaline bodies are poorly understood despite their apparent necessity for the proper functioning of haustoria. Reported here is a cell wall-focused immunohistochemical study of the hyaline bodies of three species from the ecologically important clade of rhinanthoid Orobanchaceae.Methods
Haustoria collected from laboratory-grown and field-collected plants of Rhinanthus minor, Odontites vernus and Melampyrum pratense attached to various hosts were immunolabelled for cell wall matrix glycans and glycoproteins using specific monoclonal antibodies (mAbs).Key Results
Hyaline body cell wall architecture differed from that of the surrounding parenchyma in all species investigated. Enrichment in arabinogalactan protein (AGP) epitopes labelled with mAbs LM2, JIM8, JIM13, JIM14 and CCRC-M7 was prominent and coincided with reduced labelling of de-esterified homogalacturonan with mAbs JIM5, LM18 and LM19. Furthermore, paramural bodies, intercellular deposits and globular ergastic bodies composed of pectins, xyloglucans, extensins and AGPs were common. In Rhinanthus they were particularly abundant in pairings with legume hosts. Hyaline body cells were not in direct contact with haustorial xylem, which was surrounded by a single layer of paratracheal parenchyma with thickened cell walls abutting the xylem.Conclusions
The distinctive anatomy and cell wall architecture indicate hyaline body specialization. Altered proportions of AGPs and pectins may affect the mechanical properties of hyaline body cell walls. This and the association with a transfer-like type of paratracheal parenchyma suggest a role in nutrient translocation. Organelle-rich protoplasts and the presence of exceptionally profuse intra- and intercellular wall materials when attached to a nitrogen-fixing host suggest subsequent processing and transient storage of nutrients. AGPs might therefore be implicated in nutrient transfer and metabolism in haustoria. 相似文献85.
Katrin S. Blum Sinem Karaman Steven T. Proulx Alexandra M. Ochsenbein Paola Luciani Jean-Christophe Leroux Christian Wolfrum Michael Detmar 《PloS one》2014,9(4)
Lymphatic vessels play an essential role in intestinal lipid uptake, and impairment of lymphatic vessel function leads to enhanced adipose tissue accumulation in patients with lymphedema and in genetic mouse models of lymphatic dysfunction. However, the effects of obesity on lymphatic function have been poorly studied. We investigated if and how adipose tissue accumulation influences lymphatic function. Using a lymphatic specific tracer, we performed in vivo near-infrared (NIR) imaging to assess the function of collecting lymphatic vessels in mice fed normal chow or high-fat diet (HFD). Histological and whole mount analyses were performed to investigate the morphological changes in initial and the collecting lymphatic vessels. HFD was associated with impaired collecting lymphatic vessel function, as evidenced by reduced frequency of contractions and diminished response to mechanostimulation. Moreover, we found a significant negative correlation between collecting lymphatic vessel function and body weight. Whole mount analyses showed an enlargement of contractile collecting lymphatic vessels of the hind limb. In K14-VEGF-C mice, HFD resulted in a reduced spreading of the tracer within dermal lymphatic vessels. These findings indicate that adipose tissue expansion due to HFD leads to a functional impairment of the lymphatic vasculature, predominantly in collecting lymphatic vessels. 相似文献
86.
87.
An empirical test of the relative and combined effects of land‐cover and climate change on local colonization and extinction
下载免费PDF全文
![点击此处可从《Global Change Biology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Land‐cover and climate change are two main drivers of changes in species ranges. Yet, the majority of studies investigating the impacts of global change on biodiversity focus on one global change driver and usually use simulations to project biodiversity responses to future conditions. We conduct an empirical test of the relative and combined effects of land‐cover and climate change on species occurrence changes. Specifically, we examine whether observed local colonization and extinctions of North American birds between 1981–1985 and 2001–2005 are correlated with land‐cover and climate change and whether bird life history and ecological traits explain interspecific variation in observed occurrence changes. We fit logistic regression models to test the impact of physical land‐cover change, changes in net primary productivity, winter precipitation, mean summer temperature, and mean winter temperature on the probability of Ontario breeding bird local colonization and extinction. Models with climate change, land‐cover change, and the combination of these two drivers were the top ranked models of local colonization for 30%, 27%, and 29% of species, respectively. Conversely, models with climate change, land‐cover change, and the combination of these two drivers were the top ranked models of local extinction for 61%, 7%, and 9% of species, respectively. The quantitative impacts of land‐cover and climate change variables also vary among bird species. We then fit linear regression models to test whether the variation in regional colonization and extinction rate could be explained by mean body mass, migratory strategy, and habitat preference of birds. Overall, species traits were weakly correlated with heterogeneity in species occurrence changes. We provide empirical evidence showing that land‐cover change, climate change, and the combination of multiple global change drivers can differentially explain observed species local colonization and extinction. 相似文献
88.
89.
90.