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41.
Selective sweeps of variation caused by fixation of major genes may have a dramatic impact on the genetic gain from background polygenic variation, particularly in the genome regions closely linked to the major gene. The response to selection can be restrained because of the reduced selection intensity and the reduced effective population size caused by the increase in frequency of the major gene. In the context of a selected population where fixation of a known major gene is desired, the question arises as to which is the optimal path of increase in frequency of the gene so that the selective sweep of variation resulting from its fixation is minimized. Using basic theoretical arguments we propose a frequency path that maximizes simultaneously the effective population size applicable to the selected background and the selection intensity on the polygenic variation by minimizing the average squared selection intensity on the major gene over generations up to a given fixation time. We also propose the use of mating between carriers and non-carriers of the major gene, in order to promote the effective recombination between the major gene and its linked polygenic background. Using a locus-based computer simulation assuming different degrees of linkage, we show that the path proposed is more effective than a similar path recently published, and that the combination of the selection and mating methods provides an efficient way to palliate the negative effects of a selective sweep. 相似文献
42.
Simón O Palma L Williams T López-Ferber M Caballero P 《Journal of invertebrate pathology》2012,109(1):117-126
The Nicaraguan population of Spodoptera frugiperda multiple nucleopolyhedrovirus, SfMNPV-NIC, is structured as a mixture of nine genotypes (A-I). Occlusion bodies (OBs) of SfMNPV-C, -D and -G pure genotypes are incapable of oral transmission; a phenotype which in SfMNPV-C and -D is due to the absence of pif1 and pif2 genes. The complete sequence of the SfMNPV-G genome was determined to identify possible factors involved in this phenotype. Deletions of 4860 bp (22,366-27,225) and 60 bp (119,759-119,818) were observed in SfMNPV-G genome compared with that of the predominant complete genotype SfMNPV-B (132,954 bp). However no genes homologous to previously described per os infectivity factors were located within the deleted sequences. Significant differences were detected in the nucleotide sequence in sf58 gene (unknown function) that produced changes in the amino acid sequence and the predicted secondary structure of the corresponding protein. This gene is conserved only in lepidopteran baculoviruses (alpha- and betabaculoviruses). To determine the role of sf58 in peroral infectivity a deletion mutant was constructed using bacmid technology. OBs of the deletion mutant (Sf58null) were not orally infectious for S. frugiperda larvae, whereas Sf58null rescue virus OBs recovered oral infectivity. Sf58null DNA and occlusion derived virions (ODVs) were as infective as SfMNPV bacmid DNA and ODVs in intrahemocelically infected larvae or cell culture, indicating that defects in ODV or OB morphogenesis were not involved in the loss of peroral infectivity. Addition of optical brightener or the presence of the orally infectious SfMNPV-B OBs in mixtures with SfMNPV-G OBs did not recover Sf58null OB infectivity. According to these results sf58 is a new per os infectivity factor present only in lepidopteran baculoviruses. 相似文献
43.
Santos PL Costa RV Braz JM Santos LF Batista AC Vasconcelos CR Rangel MR Ribeiro de Jesus A de Moura TR Leopoldo PT Almeida RP 《Nitric oxide》2012,27(1):67-71
Nitric oxide (NO) plays an important role as a leishmanicidal agent in murine macrophages. NO resistant Escherichia coli and Mycobacterium tuberculosis have been associated with poor outcomes of their resulting diseases. NO resistant Leishmania braziliensis has also been identified and exacerbates the clinical course of human leishmaniasis. We report, for the first time, natural resistance of Leishmania chagasi promastigotes to NO. These parasites were isolated from humans and dogs with visceral leishmaniasis. We also demonstrate that this resistance profile was associated with a greater survival capacity and a greater parasite burden in murine macrophages, independent of activation and after activation by IFN-γ and LPS. 相似文献
44.
NG Clack DH O'Connor D Huber L Petreanu A Hires S Peron K Svoboda EW Myers 《PLoS computational biology》2012,8(7):e1002591
We have developed software for fully automated tracking of vibrissae (whiskers) in high-speed videos (>500 Hz) of head-fixed, behaving rodents trimmed to a single row of whiskers. Performance was assessed against a manually curated dataset consisting of 1.32 million video frames comprising 4.5 million whisker traces. The current implementation detects whiskers with a recall of 99.998% and identifies individual whiskers with 99.997% accuracy. The average processing rate for these images was 8 Mpx/s/cpu (2.6 GHz Intel Core2, 2 GB RAM). This translates to 35 processed frames per second for a 640 px×352 px video of 4 whiskers. The speed and accuracy achieved enables quantitative behavioral studies where the analysis of millions of video frames is required. We used the software to analyze the evolving whisking strategies as mice learned a whisker-based detection task over the course of 6 days (8148 trials, 25 million frames) and measure the forces at the sensory follicle that most underlie haptic perception. 相似文献
45.
A major issue faced by breeders is how to effectively manage adverse correlations in breeding programs. We present results
of a Monte Carlo allele-based simulation of the changes in response and variance of response under adverse genetic correlations
by using the examples of two contrasting selection methods: the ‘Smith-Hazel’ selection index (SH) and independent culling
(IC). We assumed several gene models, which included linkage and antagonistic pleiotropy as the primary drivers of adverse
genetic correlations. The different behaviors of these selection methods allowed us to identify the mechanism behind the generation
of uncertainty under antagonistic trait selection: IC had the properties of stabilizing selection, while SH behaved more similar
to disruptive selection. Although SH outperformed IC in terms of genetic gain, this advantage happened at the cost of higher
variance of response and loss of heterozygosity. Using an optimum selection algorithm (OS) to prevent the loss of heterozygosity
through a constraint on inbreeding in SH/OS increased marginally the reliability, remaining still below that of IC under equal
conditions. However, SH/OS had lower inbreeding (ΔF) than IC for equivalent levels of genetic gain, so a compromise between
high selection reliability, low ΔF, and gain must be made by a breeder under antagonistic trait selection even with the use
of optimization tools. 相似文献
46.
Michael Stoehr Alvin Yanchuk Chang-Yi Xie Leopoldo Sanchez 《Tree Genetics & Genomes》2008,4(2):193-200
Sublines are used in the third-generation breeding and testing of coastal Douglas-fir in British Columbia, with the original
intent of selecting only one genotype per subline for production populations (e.g., seed orchards) to eliminate relatedness
among parents (therein called “1/SL”). We evaluated three additional selection scenarios that did not consider the subline
structure. One of the scenarios strictly selected on the basis of the highest breeding values of the trees (“TOP”); another
scenario used the TOP selections, but assigned the number of ramets per selection proportionally to the selection breeding
value (“LIND”); lastly, a simulated annealing technique was applied to maximize gain under explicit constraints on coancestry
(“OPTS”). All three alternative selection scenarios resulted in some relatedness and coancestry among selections, but the
last two provided increases in average breeding values compared to those obtained by the 1/SL scenario. Effective population
sizes (and consequently inbreeding coefficients) varied among the three selection scenarios. Effects of the various selections
on merchantable volume at rotation age were determined using a linear regression model based on an individual tree model (TASS),
which was first run to determine the relationship between merchantable volume and inbreeding (f). LIND and TOP selections yielded the highest breeding values but, due to the increased coancestry among selections, paid
a penalty in the merchantable volume determination. OPTS maximized merchantable volume at rotation age 60 after including
more than 13 selections with an increase of around 3% over that obtained by the 1/SL selection scenario, with an associated
increase in Ne of 50%. Other implications of the three alternative selection scenarios are discussed. 相似文献
47.
Helio Rodrigues da Silva Javier Bustamante Mamani Mariana Penteado Nucci Leopoldo Penteado Nucci rea Tiemi Kondo Daianne Maciely Carvalho Fantacini Lucas Eduardo Botelho de Souza Virginia Pican o-Castro Dimas Tadeu Covas Jos Mauro Kutner Fernando Anselmo de Oliveira Nelson Hamerschlak Lionel Fernel Gamarra 《World journal of stem cells》2019,11(2):100-123
48.
Lack of CD27-CD45RA-V gamma 9V delta 2+ T cell effectors in immunocompromised hosts and during active pulmonary tuberculosis. 总被引:5,自引:0,他引:5
Cristiana Gioia Chiara Agrati Rita Casetti Cristiana Cairo Giovanna Borsellino Luca Battistini Giorgio Mancino Delia Goletti Vittorio Colizzi Leopoldo P Pucillo Fabrizio Poccia 《Journal of immunology (Baltimore, Md. : 1950)》2002,168(3):1484-1489
In humans, the circulating pool of mycobacteria-reactive Vgamma9Vdelta2+ T cells is expanded with age and may contribute to Mycobacterium tuberculosis immunosurveillance. We observed that two subsets of Vgamma9Vdelta2+ T cells could be identified on the basis of CD27 expression in immunocompetent adults, showing that functionally differentiated gammadelta T cells have lost CD27 expression. In contrast, the CD27-CD45RA-Vgamma9Vdelta2+ T cell subset of effector cells was absent in cord blood cells from healthy newborns and lacking in the peripheral blood from HIV-infected patients. Moreover, circulating Vgamma9Vdelta2+ T cell effectors were significantly reduced in patients with acute pulmonary tuberculosis, resulting in a reduced frequency of IFN-gamma-producing cells after stimulation with nonpeptidic mycobacterial ligands. These observations indicate that monitoring and boosting gammadelta T cell effectors could be clinically relevant both in immunocompromised hosts and during active tuberculosis disease. 相似文献
49.
Kristin Palmqvist Lena Dahlman Fernando Valladares Anders Tehler Leopoldo G. Sancho Jan-Eric Mattsson 《Oecologia》2002,133(3):295-306
Aiming to investigate whether a carbon-to-nitrogen equilibrium model describes resource allocation in lichens, net photosynthesis (NP), respiration (R), concentrations of nitrogen (N), chlorophyll (Chl), chitin and ergosterol were investigated in 75 different lichen associations collected in Antarctica, Arctic Canada, boreal Sweden, and temperate/subtropical forests of Tenerife, South Africa and Japan. The lichens had various morphologies and represented seven photobiont and 41 mycobiont genera. Chl a, chitin and ergosterol were used as indirect markers of photobiont activity, fungal biomass and fungal respiration, respectively. The lichens were divided into three groups according to photobiont: (1) species with green algae, (2) species with cyanobacteria, and (3) tripartite species with green algal photobionts and cyanobacteria in cephalodia. Across species, thallus N concentration ranged from 1 to 50 mg g-1 dry wt., NP varied 50-fold, and R 10-fold. In average, green algal lichens had the lowest, cyanobacterial Nostoc lichens the highest and tripartite lichens intermediate N concentrations. All three markers increased with thallus N concentration, and lichens with the highest Chl a and N concentrations had the highest rates of both P and R. Chl a alone accounted for ca. 30% of variation in NP and R across species. On average, the photosynthetic efficiency quotient [KF=(NPmax+R)/R)] ranged from 2.4 to 8.6, being higher in fruticose green algal lichens than in foliose Nostoc lichens. The former group invested more N in Chl a and this trait increased NPmax while decreasing R. In general terms, the investigated lichens invested N resources such that their maximal C input capacity matched their respiratory C demand around a similar (positive) equilibrium across species. However, it is not clear how this apparent optimisation of resource use is regulated in these symbiotic organisms. 相似文献
50.
Allan T. Green Mark Schlensog Leopoldo G. Sancho Barbro J. Winkler Fraser D. Broom Burkhard Schroeter 《Oecologia》2002,130(2):191-198
Photosystem activity status of the green algal (Pseudocyphellaria lividofusca) and cyanobacterial (P. knightii) components of a photosymbiodeme were continuously monitored in the field over a period of 35 days. The photosymbiodeme grew on a Nothofagus menziesii tree at Lake Waikaremoana, Urewera National Park, North Island, New Zealand. Two Mini-PAM fluorometers were placed so that the chlorophyll a fluorescence, temperature and PPFD (photosynthetically active photon flux density) could be recorded every 30 min for green algal and cyanobacterial parts of the thallus. Microclimate conditions were also recorded with a datalogger. The study confirmed the already known ability of green algal lichens to reactivate from high humidity alone whilst cyanobacterial species need liquid water, here obtained from rainfall. The photosystems of P. lividofusca were activated on every day and positive ETR (relative electron transport rate) occurred on all but 3 days. Activation level depended on the overnight relative humidity. P. knightii was activated and had positive ETR on only 13 days when rainfall had occurred. Both species were mostly inactive above 12°C but differed at low temperatures. P. knightii showed no activation at very low temperatures, -2 to 0°C, since these only occurred on clear, rain-free nights. PPFD was always very low, mostly below 80 µmol m-2 s-1, and both species were inactive at higher PPFD. The three-dimensional structure of the thallus seemed to contribute to the hydration. The cyanobacterial sectors were more appressed to the trunk and needed substantial rainfall to rewet whereas the green algal lobes were more distant from the trunk and this probably caused more rapid desiccation as well as lower temperatures. It is suggested that the longer active periods for photosynthesis by P. lividofusca are balanced by several factors: first, depressed net photosynthesis at high thallus water contents after rainfall, a feature not shown by P. knightii; second, possible lower maximal net photosynthetic rates; and third, the possibility of greater respiratory rates when thalli have been hydrated by high relative humidity. There is little evidence for high PPFD differently affecting the photosymbiodeme components since sustained, high PPFD did not occur. It has been reported that the photosystems of cyanobacterial species from photosymbiodemes can reactivate at high relative humidity but the results obtained here suggest that it is not ecologically significant. 相似文献