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471.
Marie-Eve Gauthier Line Rochefort Leonie Nadeau Sandrine Hugron Bin Xu 《Wetlands Ecology and Management》2018,26(4):475-487
Peatlands are abundant in the boreal region of Canada but little is known about their restoration on oil sands well pads. The goal of this study is to compare the reintroduction of different peatland plant communities and substrate amendments/decompaction in order to rehabilitate peatland vegetation on former in situ well pads constructed in wetlands. One field experiment tested which peatland plant communities (Shrubby Rich Fen, Treed Rich Fen) would best colonize different substrates (sawdust, clay loam, mix sawdust-clay, peat, surface roughness). We found that the moss layer transfer technique (MLTT) facilitated the establishment of peatland communities on residual mineral soil used to construct the pad, when shaved back to an average water level of the surrounding wetland. The choice of peatland plant community is key to the introduction of bryophytes. Peat amendment facilitated the establishment of plants, whereas soil decompaction had no effect. The MLTT is a promising approach to restore fen plants on well pads. We recommend a scale-up experiment for a whole well site to test the validity of MLTT along different pad removal techniques. 相似文献
472.
Bryony J. Palmer Leonie E. Valentine Cheryl A. Lohr Gergana N. Daskalova Richard J. Hobbs 《Ecology and evolution》2021,11(6):2596
Digging and burrowing mammals modify soil resources, creating shelter for other animals and influencing vegetation and soil biota. The use of conservation translocations to reinstate the ecosystem functions of digging and burrowing mammals is becoming more common. However, in an increasingly altered world, the roles of translocated populations, and their importance for other species, may be different. Boodies (Bettongia lesueur), a commonly translocated species in Australia, construct extensive warrens, but how their warrens affect soil properties and vegetation communities is unknown. We investigated soil properties, vegetation communities, and novel ecosystem elements (specifically non‐native flora and fauna) on boodie warrens at three translocation sites widely distributed across the species’ former range. We found that soil moisture and most soil nutrients were higher, and soil compaction was lower, on warrens in all sites and habitat types. In contrast, there were few substantial changes to vegetation species richness, cover, composition, or productivity. In one habitat type, the cover of shrubs less than 1 m tall was greater on warrens than control plots. At the two sites where non‐native plants were present, their cover was greater, and they were more commonly found on boodie warrens compared to control plots. Fourteen species of native mammals and reptiles were recorded using the warrens, but, where they occurred, the scat of the non‐native rabbit (Oryctolagus cuniculus) was also more abundant on the warrens. Together, our results suggest that translocated boodie populations may be benefiting both native and non‐native flora and fauna. Translocated boodies, through the construction of their warrens, substantially alter the sites where they are released, but this does not always reflect their historic ecosystem roles. 相似文献
473.
Lauren M. Halstead Duncan R. Sutherland Leonie E. Valentine Anthony R. Rendall Amy L. Coetsee Euan G. Ritchie 《Austral ecology》2020,45(1):97-108
Digging mammals are often considered ecosystem engineers, as they affect important properties of soils and in turn nutrient exchange, vegetation dynamics and habitat quality. Returning such species, and their functions, to areas from where they have been extirpated could help restore degraded landscapes and is increasingly being trialled as a conservation tool. Studies examining the effects of digging mammals have largely been from arid and semi‐arid environments, with little known about their impacts and importance in mesic systems. To address this knowledge gap, we investigated the ecological role of a recently introduced population of eastern barred bandicoots (Perameles gunnii) on Churchill Island, Victoria, south‐eastern Australia, from which all digging mammals have been lost. We quantified the annual rate of soil turnover by estimating the number of foraging pits bandicoots created in 100‐m2 plots over a 24‐h period. Foraging pit counts could not be completed in each season, and the overall turnover estimate assumes that autumn/winter months represent turnover rates for the entire year; however, this is likely to fluctuate between seasons. Ten fresh and ten old pits were compared to paired undug control sites to quantify the effect soil disturbance had on soil hydrophobicity, moisture content and soil strength. Plots contained between zero and 64 new foraging pits each day. We estimated that an individual eastern barred bandicoot digs ~487 (95% CI = 416–526) small foraging pits per night, displacing ~13.15 kg (95% CI = 11.2–14.2 kg) of soil, equating to ~400 kg (95% CI = 341–431 kg) of soil in a winter month. Foraging pits were associated with decreased soil compaction and increased soil moisture along the foraging pit profile. Eastern barred bandicoots likely play an important role in ecosystems through their effects on soil, which adds to an increasing body of knowledge suggesting restoration of ecosystems, via the return of ecosystem engineers and their functions, holds much promise for conserving biodiversity and ecological function. 相似文献
474.
Hybrid Sterility over Tens of Meters Between Ecotypes Adapted to Serpentine and Non-Serpentine Soils
Leonie C. Moyle Mia Levine Maureen L. Stanton Jessica W. Wright 《Evolutionary biology》2012,39(2):207-218
The development of hybrid sterility is an important step in the process of speciation, however the role of adaptive evolution in triggering these postzygotic barriers is poorly understood. We show that, in the California endemic plant Collinsia sparsiflora, ecotypic adaptation to two distinct soil types is associated with the expression of intrinsic F1 hybrid sterility between ecotypes, over spatial scales of less than 1?km. First, we show that hybrids between soil-adapted ecotypes are less fertile than hybrids within soil ecotypes. Second, we show that between-ecotype postzygotic incompatibility is insensitive to soil growth environment, and can therefore operate under conditions relevant to both ecotypes in the wild. Third, we confirm there is little genetic differentiation between ecotypes using molecular markers, indicating that these postzygotic barriers are recently evolved. Finally, we explore specific soil attributes that might be the source of selective differentiation that confers hybrid sterility. Our results indicate that hybrid barriers are developing in response to strong adaptive differentiation between adjacent and very recently diverged lineages, despite likely ongoing gene exchange. 相似文献
475.
Leonie K. Ashman 《Cellular immunology》1984,87(2):613-625
The mitogenicity of 12-O-tetradecanoyl phorbol-13-acetate (TPA) for normal human peripheral blood mononuclear cells was investigated. TPA was a weak mitogen giving simulation indices in the range 2.5 to 10.5 at the optimum concentration (10 ng/ml) compared with 39 to 95 for phytohemagglutinin (PHA) at its optimum concentration (1 μg/ml). No absolute requirement for a comitogen could be demonstrated, however TPA and PHA were synergistic in their action at low concentrations, and additive at optimum concentrations. Cell fractionation by rosetting with sheep erythrocytes showed that most of the proliferative response to TPA occurred in the T-cell fraction, however some proliferation of non-T cells was also observed. Surface marker studies showed that this could not have been due to residual T cells in the non-T fraction. A small number of monocytes was required for optimal proliferation of T cells in response to TPA. After a 3-day incubation with mitogen, the responding cell populations were tested for binding of a range of antibodies specific for T-cell (OKT3, OKT4, OKT8, and OKT11), “natural killer” (NK) cell (anti-Leu-7), monocyte (FMC17), and B-cell (anti-human immunoglobulin) surface markers. These experiments indicated that the responding cell types were T cells and B cells, but not NK cells or monocytes. Marked modulation of the antigen detected by OKT4, and to a lesser extent that detected by OKT3, in the presence of TPA precluded determination of which subpopulations of T cells proliferated in response to TPA. TPA was also tested for its ability to “maintain” activated T-cell blasts in a standard assay for interleukin 2 (IL-2). Mitogen-activated T cells were strongly responsive to TPA in this assay, but progressively lost responsiveness when maintained in crude IL-2 for about 2 weeks. Thus TPA does not have “maintenance” (i.e., IL-2-like) activity. However, small amounts of TPA acted synergistically with PHA in maintaining blast populations which were not responsive to TPA alone. This illustrates the importance of using long term IL-2-dependent cell lines for quantitation of IL-2 in supernatants prepared by stimulating T cells with these agents. 相似文献
476.
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478.
Leonie Smeenk Meinrad Busslinger Leonie Smeenk Maria Fischer Sabine Jurado Markus Jaritz Anna Azaryan Barbara Werner Mareike Roth Johannes Zuber Martin Stanulla Monique L den Boer Charles G Mullighan Sabine Strehl Meinrad Busslinger 《The EMBO journal》2017,36(6):718-735
PAX5 is a tumor suppressor in B-ALL, while the role of PAX5 fusion proteins in B-ALL development is largely unknown. Here, we studied the function of PAX5-ETV6 and PAX5-FOXP1 in mice expressing these proteins from the Pax5 locus. Both proteins arrested B-lymphopoiesis at the pro-B to pre-B-cell transition and, contrary to their proposed dominant-negative role, did not interfere with the expression of most regulated Pax5 target genes. Pax5-Etv6, but not Pax5-Foxp1, cooperated with loss of the Cdkna2a/b tumor suppressors in promoting B-ALL development. Regulated Pax5-Etv6 target genes identified in these B-ALLs encode proteins implicated in pre-B-cell receptor (BCR) signaling and migration/adhesion, which could contribute to the proliferation, survival, and tissue infiltration of leukemic B cells. Together with similar observations made in human PAX5-ETV6+ B-ALLs, these data identified PAX5-ETV6 as a potent oncoprotein that drives B-cell leukemia development. 相似文献
479.
Lucy A.L. Tainton-Heap Leonie C. Kirszenblat Eleni T. Notaras Martyna J. Grabowska Rhiannon Jeans Kai Feng Paul J. Shaw Bruno van Swinderen 《Current biology : CB》2021,31(3):578-590.e6
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480.