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991.
Ana C. M. Malhado Rafael S. D. de Azevedo Peter A. Todd Ana M. C. Santos Nídia N. Fabré Vandick S. Batista Leonardo J. G. Aguiar Richard J. Ladle 《Biotropica》2014,46(1):6-13
The presence of researchers from Western (i.e., developed world) institutions in Amazonia has frequently been contentious due to fears of ‘scientific imperialism’ or suspicions that they may be exerting undue influence over research agendas and knowledge production to the detriment of local researchers. Such negative perceptions are widespread, but not well substantiated. A more nuanced understanding of these issues requires information on who is conducting research in Amazonia and how knowledge production has changed over time. We performed a bibliometric analysis on Thomson Reuters'ISI Web of Science of all research articles about the Amazon published in three time periods: 1986–1989, 1996–1999, and 2006–2009. We found that the number of articles published and the diversity of countries involved in Amazonian research increased dramatically over the three time periods. The representation of several Amazonian countries—especially Brazil—increased, while the proportion of articles without a single author from an Amazonian country also increased. The results indicate that the research capacity of Amazonian countries has increased, but that leadership of high‐impact projects may still largely reside with researchers from developed countries. 相似文献
992.
To evaluate the quantitative and qualitative changes in amino acids related to internal nitrogen content and growth rate of Ulva ohnoi, the supply of nitrogen to outdoor cultures of the seaweed was manipulated by simultaneously varying water nitrogen concentrations and renewal rate. Both internal nitrogen content and growth rate varied substantially, and the quantitative and qualitative changes in amino acids were described in the context of three internal nitrogen states: nitrogen‐limited, metabolic, and luxury. The nitrogen limited state was defined by increases in all amino acids with increasing nitrogen content and growth up until 1.2% internal nitrogen. The metabolic nitrogen state was defined by increases in all amino acids with increasing internal nitrogen content up to 2.6%, with no increases in growth rate. Luxury state was defined by internal nitrogen content above 2.6%, which occurred only when nitrogen availability was high but growth rates were reduced. In this luxury circumstance, excess nitrogen was accumulated as free amino acids, in two phases. The first phase was distinguished by a small increase in the majority of amino acids up to ≈3.3% internal nitrogen, and the second by a large increase in glutamic acid, glutamine, and arginine up to 4.2% internal nitrogen. These results demonstrate that the relationship between internal nitrogen content and amino acid quality is dynamic but predictable, and could be used for the selective culture of seaweeds. 相似文献
993.
Cecilia Ferrantini Raffaele Coppini Leonardo Sacconi Benedetta Tosi Mei Luo Zhang Guo Liang Wang Ewout de Vries Ernst Hoppenbrouwers Francesco Pavone Elisabetta Cerbai Chiara Tesi Corrado Poggesi Henk E.D.J. ter Keurs 《The Journal of general physiology》2014,143(6):783-797
Action potential–driven Ca2+ currents from the transverse tubules (t-tubules) trigger synchronous Ca2+ release from the sarcoplasmic reticulum of cardiomyocytes. Loss of t-tubules has been reported in cardiac diseases, including heart failure, but the effect of uncoupling t-tubules from the sarcolemma on cardiac muscle mechanics remains largely unknown. We dissected intact rat right ventricular trabeculae and compared force, sarcomere length, and intracellular Ca2+ in control trabeculae with trabeculae in which the t-tubules were uncoupled from the plasma membrane by formamide-induced osmotic shock (detubulation). We verified disconnection of a consistent fraction of t-tubules from the sarcolemma by two-photon fluorescence imaging of FM4-64–labeled membranes and by the absence of tubular action potential, which was recorded by random access multiphoton microscopy in combination with a voltage-sensitive dye (Di-4-AN(F)EPPTEA). Detubulation reduced the amplitude and prolonged the duration of Ca2+ transients, leading to slower kinetics of force generation and relaxation and reduced twitch tension (1 Hz, 30°C, 1.5 mM [Ca2+]o). No mechanical changes were observed in rat left atrial trabeculae after formamide shock, consistent with the lack of t-tubules in rodent atrial myocytes. Detubulation diminished the rate-dependent increase of Ca2+-transient amplitude and twitch force. However, maximal twitch tension at high [Ca2+]o or in post-rest potentiated beats was unaffected, although contraction kinetics were slower. The ryanodine receptor (RyR)2 Ca-sensitizing agent caffeine (200 µM), which increases the velocity of transverse Ca2+ release propagation in detubulated cardiomyocytes, rescued the depressed contractile force and the slower twitch kinetics of detubulated trabeculae, with negligible effects in controls. We conclude that partial loss of t-tubules leads to myocardial contractile abnormalities that can be rescued by enhancing and accelerating the propagation of Ca2+-induced Ca2+ release to orphan RyR2 clusters. 相似文献
994.
Martha Elba Gonzalez-Mejia Enrique Torres-Rasgado Leonardo M Porchia Hilda Rosas Salgado José-Luis Totolhua Arturo Ortega Luisa Clara Regina Hernández-Kelly Guadalupe Ruiz-Vivanco Blanca G Báez-Duarte Ricardo Pérez-Fuentes 《Memórias do Instituto Oswaldo Cruz》2014,109(2):174-181
Chagas disease, caused by Trypanosoma cruzi, represents an endemic
among Latin America countries. The participation of free radicals, especially nitric
oxide (NO), has been demonstrated in the pathophysiology of seropositive individuals
with T. cruzi. In Chagas disease, increased NO contributes to the
development of cardiomyopathy and megacolon. Metallothioneins (MTs) are efficient
free radicals scavengers of NO in vitro and in vivo. Here, we developed a murine
model of the chronic phase of Chagas disease using endemic T. cruzi
RyCH1 in BALB/c mice, which were divided into four groups: infected non-treated
(Inf), infected N-monomethyl-L-arginine treated (Inf L-NAME), non-infected L-NAME
treated and non-infected vehicle-treated. We determined blood parasitaemia and NO
levels, the extent of parasite nests in tissues and liver MT-I expression levels. It
was observed that NO levels were increasing in Inf mice in a time-dependent manner.
Inf L-NAME mice had fewer T. cruzi nests in cardiac and skeletal
muscle with decreased blood NO levels at day 135 post infection. This affect was
negatively correlated with an increase of MT-I expression (r = -0.8462, p <
0.0001). In conclusion, we determined that in Chagas disease, an unknown inhibitory
mechanism reduces MT-I expression, allowing augmented NO levels. 相似文献
995.
Felipe H. Coutinho Cynthia B. Silveira Leonardo H. Pinto Gigliola R. B. Salloto Alexander M. Cardoso Orlando B. Martins Ricardo P. Vieira Maysa M. Clementino 《Microbial ecology》2014,68(3):441-452
Bacterial resistance to antibiotics has become a public health issue. Over the years, pathogenic organisms with resistance traits have been studied due to the threat they pose to human well-being. However, several studies raised awareness to the often disregarded importance of environmental bacteria as sources of resistance mechanisms. In this work, we analyze the diversity of antibiotic-resistant bacteria occurring in aquatic environments of the state of Rio de Janeiro, Brazil, that are subjected to distinct degrees of anthropogenic impacts. We access the diversity of aquatic bacteria capable of growing in increasing ampicillin concentrations through 16S rRNA gene libraries. This analysis is complemented by the characterization of antibiotic resistance profiles of isolates obtained from urban aquatic environments. We detect communities capable of tolerating antibiotic concentrations up to 600 times higher than the clinical levels. Among the resistant organisms are included potentially pathogenic species, some of them classified as multiresistant. Our results extend the knowledge of the diversity of antibiotic resistance among environmental microorganisms and provide evidence that the diversity of drug-resistant bacteria in aquatic habitats can be influenced by pollution. 相似文献
996.
Gabriele Andrea Lugli Christian Milani Francesca Turroni Sabrina Duranti Chiara Ferrario Alice Viappiani Leonardo Mancabelli Marta Mangifesta Bernard Taminiau Véronique Delcenserie Douwe van Sinderen Marco Ventura 《Applied and environmental microbiology》2014,80(20):6383-6394
The Bifidobacterium genus currently encompasses 48 recognized taxa, which have been isolated from different ecosystems. However, the current phylogeny of bifidobacteria is hampered by the relative paucity of genotypic data. Here, we reassessed the taxonomy of this bacterial genus using genome-based approaches, which demonstrated that the previous taxonomic view of bifidobacteria contained several inconsistencies. In particular, high levels of genetic relatedness were shown to exist between particular Bifidobacterium taxa which would not justify their status as separate species. The results presented are here based on average nucleotide identity analysis involving the genome sequences for each type strain of the 48 bifidobacterial taxa, as well as phylogenetic comparative analysis of the predicted core genome of the Bifidobacterium genus. The results of this study demonstrate that the availability of complete genome sequences allows the reconstruction of a more robust bifidobacterial phylogeny than that obtained from a single gene-based sequence comparison, thus discouraging the assignment of a new or separate bifidobacterial taxon without such a genome-based validation. 相似文献
997.
Fernando Navarrete Leonardo De La Fuente 《Applied and environmental microbiology》2014,80(3):1097-1107
The bacterial plant pathogen Xylella fastidiosa produces biofilm that accumulates in the host xylem vessels, affecting disease development in various crops and bacterial acquisition by insect vectors. Biofilms are sensitive to the chemical composition of the environment, and mineral elements being transported in the xylem are of special interest for this pathosystem. Here, X. fastidiosa liquid cultures were supplemented with zinc and compared with nonamended cultures to determine the effects of Zn on growth, biofilm, and exopolysaccharide (EPS) production under batch and flow culture conditions. The results show that Zn reduces growth and biofilm production under both conditions. However, in microfluidic chambers under liquid flow and with constant bacterial supplementation (closer to conditions inside the host), a dramatic increase in biofilm aggregates was seen in the Zn-amended medium. Biofilms formed under these conditions were strongly attached to surfaces and were not removed by medium flow. This phenomenon was correlated with increased EPS production in stationary-phase cells grown under high Zn concentrations. Zn did not cause greater adhesion to surfaces by individual cells. Additionally, viability analyses suggest that X. fastidiosa may be able to enter the viable but nonculturable state in vitro, and Zn can hasten the onset of this state. Together, these findings suggest that Zn can act as a stress factor with pleiotropic effects on X. fastidiosa and indicate that, although Zn could be used as a bactericide treatment, it could trigger the undesired effect of stronger biofilm formation upon reinoculation events. 相似文献
998.
999.
1000.
Jhones Luiz de Oliveira Estefânia Vangelie Ramos Campos Mansi Bakshi P.C. Abhilash Leonardo Fernandes Fraceto 《Biotechnology advances》2014
This review article discusses the use of nanotechnology in combination with botanical insecticides in order to develop systems for pest control in agriculture. The main types of botanical insecticides are described, together with different carrier systems and their potential uses. The botanical insecticides include those based on active principles isolated from plant extracts, as well as essential oils derived from certain plants. The advantages offered by the systems are highlighted, together with the main technological challenges that must be resolved prior to future implementation of the systems for agricultural pest control. The use of botanical insecticides associated with nanotechnology offers considerable potential for increasing agricultural productivity, while at the same time reducing impacts on the environment and human health. 相似文献