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991.
April H. Hastwell Leo Corcilius James T. Williams Peter M. Gresshoff Richard J. Payne Brett J. Ferguson 《Plant, cell & environment》2019,42(1):188-197
Legumes form root nodules to house beneficial nitrogen‐fixing rhizobia bacteria. However, nodulation is resource demanding; hence, legumes evolved a systemic signalling mechanism called autoregulation of nodulation (AON) to control nodule numbers. AON begins with the production of CLE peptides in the root, which are predicted to be glycosylated, transported to the shoot, and perceived. We synthesized variants of nodulation‐suppressing CLE peptides to test their activity using petiole feeding to introduce CLE peptides into the shoot. Hydroxylated, monoarabinosylated, and triarabinosylated variants of soybean GmRIC1a and GmRIC2a were chemically synthesized and fed into recipient Pisum sativum (pea) plants, which were used due to the availability of key AON pathway mutants unavailable in soybean. Triarabinosylated GmRIC1a and GmRIC2a suppressed nodulation of wild‐type pea, whereas no other peptide variant tested had this ability. Suppression also occurred in the supernodulating hydroxyproline O‐arabinosyltransferase mutant, Psnod3, but not in the supernodulating receptor mutants, Pssym29, and to some extent, Pssym28. During our study, bioinformatic resources for pea became available and our analyses identified 40 CLE peptide‐encoding genes, including orthologues of nodulation‐suppressive CLE peptides. Collectively, we demonstrated that soybean nodulation‐suppressive CLE peptides can function interspecifically in the AON pathway of pea and require arabinosylation for their activity. 相似文献
992.
De Leo Filomena Lo Giudice Angelina Alaimo Chiara De Carlo Giusy Rappazzo Alessandro Ciro Graziano Marco De Domenico Emilio Urzì Clara 《Extremophiles : life under extreme conditions》2019,23(1):9-17
Extremophiles - The occurrence of cultivable fungi was investigated along the water column (25–2500 m depth) of four off-shore stations in the Mediterranean basin. An unexpected high... 相似文献
993.
Leo Valon Fred Etoc Amanda Remorino Florencia di?Pietro Xavier Morin Maxime Dahan Mathieu Coppey 《Biophysical journal》2015,109(9):1785-1797
Recently developed optogenetic methods promise to revolutionize cell biology by allowing signaling perturbations to be controlled in space and time with light. However, a quantitative analysis of the relationship between a custom-defined illumination pattern and the resulting signaling perturbation is lacking. Here, we characterize the biophysical processes governing the localized recruitment of the Cryptochrome CRY2 to its membrane-anchored CIBN partner. We develop a quantitative framework and present simple procedures that enable predictive manipulation of protein distributions on the plasma membrane with a spatial resolution of 5 μm. We show that protein gradients of desired levels can be established in a few tens of seconds and then steadily maintained. These protein gradients can be entirely relocalized in a few minutes. We apply our approach to the control of the Cdc42 Rho GTPase activity. By inducing strong localized signaling perturbation, we are able to monitor the initiation of cell polarity and migration with a remarkable reproducibility despite cell-to-cell variability. 相似文献
994.
Thomas J. Cahill Vincenzo Leo Matthew Kelly Alexander Stockenhuber Nolan W. Kennedy Leyuan Bao Grazia Cereghetti Andrew R. Harper Gabor Czibik Chunyan Lao Mohamed Bellahcene Violetta Steeples Safar Ghaffari Arash Yavari Alice Mayer Joanna Poulton David J. P. Ferguson Luca Scorrano Nishani T. Hettiarachchi Chris Peers John Boyle R. Blake Hill Alison Simmons Hugh Watkins T. Neil Dear Houman Ashrafian 《The Journal of biological chemistry》2015,290(43):25907-25919
We have reported previously that a missense mutation in the mitochondrial fission gene Dynamin-related protein 1 (Drp1) underlies the Python mouse model of monogenic dilated cardiomyopathy. The aim of this study was to investigate the consequences of the C452F mutation on Drp1 protein function and to define the cellular sequelae leading to heart failure in the Python monogenic dilated cardiomyopathy model. We found that the C452F mutation increased Drp1 GTPase activity. The mutation also conferred resistance to oligomer disassembly by guanine nucleotides and high ionic strength solutions. In a mouse embryonic fibroblast model, Drp1 C452F cells exhibited abnormal mitochondrial morphology and defective mitophagy. Mitochondria in C452F mouse embryonic fibroblasts were depolarized and had reduced calcium uptake with impaired ATP production by oxidative phosphorylation. In the Python heart, we found a corresponding progressive decline in oxidative phosphorylation with age and activation of sterile inflammation. As a corollary, enhancing autophagy by exposure to a prolonged low-protein diet improved cardiac function in Python mice. In conclusion, failure of Drp1 disassembly impairs mitophagy, leading to a downstream cascade of mitochondrial depolarization, aberrant calcium handling, impaired ATP synthesis, and activation of sterile myocardial inflammation, resulting in heart failure. 相似文献
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Selection becomes visible: enforced sexual dimorphism caused by sexual selection in the weevil Rhopalapion longirostre (Olivier 1807) (Coleoptera: Curculionoidea: Brentidae) 下载免费PDF全文
Gertha Wilhelm Stephan Handschuh John Plant Hans Leo Nemeschkal 《Biological journal of the Linnean Society. Linnean Society of London》2015,115(1):38-47
Extremely divergent traits between males and females are often the result of different requirements and behaviours of the sexes and will evolve relatively rapidly under selection forces. Sexual dimorphism in Rhopalapion longirostre is predominately manifested in the length and structure of the rostrum. To estimate how sexual selection shapes mating success in this weevil we compared paired and unpaired individuals collected from three populations in Austria. The mating process in this species is complex and lengthy. Statistical analyses based on detailed observations of their mating behaviour revealed that matched pairs show functional affinities in body size. Females and males with larger elytra, as well as males with large overall body size, are favoured mating partners, while males that are too small have no mating success. This arrangement ensures copulation and consequently successful egg deposition. For efficient egg channel boring into the flower buds of the host plant, Alcea rosea, the extremely long female rostrum is a crucial tool. Natural selection promotes longer rostra in females whereas sexual selection favours the shorter rostra in males. The major evolutionary forces, natural and sexual selection, enhance the sexual dimorphism in this species. © 2015 The Linnean Society of London, Biological Journal of the Linnean Society, 2015, 115 , 38–47. 相似文献
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Neuronal network consists of many types of neuron and glial cells. This diversity is guaranteed by the constant cell proliferation of neuronal stem cells following stop cell cycle re‐entry, which leads to differentiation during development. Neuronal differentiation occurs mainly at the specific cell cycle phase, the G1 phase. Therefore, cell cycle exit at the G1 phase is quite an important issue in understanding the process of neuronal cell development. Recent studies have revealed that aberrant S phase re‐entry from the G1 phase often links cellular survival. In this review we discuss the different types of G1 arrest on the process of neuronal development in Drosophila. We also describe the issue that aberrant S phase entry often causes apoptosis, and the same mechanism might contribute to sensory organ defects, such as deafness. 相似文献
1000.
Kirsty Agnoli Carmen Frauenknecht Roman Freitag Stephan Schwager Christian Jenul Annette Vergunst Aurelien Carlier Leo Eberl 《Applied and environmental microbiology》2014,80(4):1340-1348
The metabolically versatile Burkholderia cepacia complex (Bcc) occupies a variety of niches, including the plant rhizosphere and the cystic fibrosis lung (where it is often fatal to the patient). Bcc members have multipartite genomes, of which the third replicon, pC3 (previously chromosome 3), has been shown to be a nonessential megaplasmid which confers virulence and both antifungal and proteolytic activity on several strains. In this study, pC3 curing was extended to cover strains of 16 of the 17 members of the Bcc, and the phenotypes conferred by pC3 were determined. B. cenocepacia strains H111, MCO-3, and HI2424 were previously cured of pC3; however, this had not proved possible in the epidemic strain K56-2. Here, we investigated the mechanism of this unexpected stability and found that efficient toxin-antitoxin systems are responsible for maintaining pC3 of strain K56-2. Identification of these systems allowed neutralization of the toxins and the subsequent deletion of K56-2pC3. The cured strain was found to exhibit reduced antifungal activity and was attenuated in both the zebrafish and the Caenorhabditis elegans model of infection. We used a PCR screening method to examine the prevalence of pC3 within 110 Bcc isolates and found that this replicon was absent in only four cases, suggesting evolutionary fixation. It is shown that plasmid pC3 increases the resistance of B. cenocepacia H111 to various stresses (oxidative, osmotic, high-temperature, and chlorhexidine-induced stresses), explaining the prevalence of this replicon within the Bcc. 相似文献