Über das Wachsthum des Epithels

Ohne Zusammenfassung     

Beitr?ge zur Brutvogelfauna der Provinz Malaga

Ohne Zusammenfassung     

Untersuchungen über den photoelektrischen Effekt in Membranen. I

Ohne Zusammenfassung     

Über die Untersuchung der Nervenfasern und Zellen mittelst Färbung derselben mit Methylenblau

Ohne Zusammenfassung     

Der Einfluss von Schilddrüsenfütterung auf Entwicklung,Wachstum und Fortpflanzung des SpeckkÄfers (Dermestes Frischii Kg)

Ohne ZusammenfassungExperimentelle Untersuchungen über die Wirkung von Wirbeltierhormonen auf Wirbellose. Von B.Romeis. 4. Mitteilung.Ausgeführt mit Unterstützung der Notgemeinschaft der deutschen Wissenschaft.     

Cellular distribution of lens epithelium-derived growth factor (LEDGF) in the rat eye: loss of LEDGF from nuclei of differentiating cells

Lens epithelium-derived growth factor (LEDGF) enhances the survival and growth of cells. To understand LEDGF's spatial localization and its putative function(s) during proliferation and differentiation, we localized LEDGF during terminal differentiation in whole rat lenses, lens epithelial cell (LEC) explants stimulated with FGF-2, and insulin, iris, human LECs with lentoids. In addition, intracellular localization of LEDGF was performed in other ocular tissues: ciliary body, retina, and cornea. We found the immunopositivity of nuclear LEDGF decreased in LECs of the equatorial region. In contrast, immunopositivity of LEDGF was detected in the cytoplasm of LECs and superficial fiber cells. After treating LEC explants with FGF-2 and insulin, which are known to be differentiating factors for LECs, the nuclei of these cells showed no LEDGF immunopositivity, but explants did express p57(kip2), a differentiation marker protein. Also, immunopositive LEDGF was not detected in the nuclei of differentiated cells, lentoid body, and corneal epithelial cells. This demonstrated that the loss of LEDGF from the nucleus may be associated with the process of terminal differentiation that might be in some way common with the biochemical mechanisms of apoptosis. The spatial and temporal distribution of LEDGF in the present study also provides a vision for further investigation as to how this protein is involved in cell fate determination.