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51.
O.?SanllorenteEmail author P.?Lorite S.?Devers F.?Ruano A.?Lenoir A.?Tinaut 《Insectes Sociaux》2012,59(3):361-368
Host and parasite distributions are crucial to understand the coevolutionary outcomes of their relationships. This comes from the fact that the distribution of a species (fragmented vs. continuous habitats) influences its dispersal opportunities. In this work, we studied the effect of the spatial distribution on dispersal and coevolution between three species of social parasite ants of the genus Rossomyrmex (one distributed in high mountains in Spain and two distributed in extended plains in Turkey and Kazakhstan) and their ant hosts Proformica. We analysed the variation at the mitochondrial gene cytochrome c oxidase (COI) to infer female dispersal for parasites as well as the cuticular hydrocarbons (CHCs) of parasites and hosts to study their coevolutionary process, given that CHCs are involved in nestmate recognition. Our genetic results revealed a surprising scarce variation at COI for the three parasite species, suggesting selective forces that prevent from mutation fixation. Therefore, COI appeared to be a poor tool to study dispersal. Furthermore, chemical results showed population differentiation for all host–parasite systems, pointing that coevolution would take place at a local scale regardless of the spatial distribution or dispersal opportunities of the counterparts. 相似文献
52.
In this paper we analyze emigration from nests by the polydomous ant Cataglyphis iberica. Social carrying of workers of this species between different nests of the colony is frequent. In Bellaterra (Barcelona,
NE Spain), we monitored field emigration of C. iberica by noting for each nest the migratory behavior of C. iberica workers and, when the nests were attacked by another ant species, Camponotus foreli, we noted the number of C. foreli workers involved in the attacks. Emigration of C. iberica from nests was highly variable. We suggest the main factor determining emigration by this species was attack by workers of
C. foreli, so emigration from C. iberica nests was much faster when harassment by C. foreli increased. The system of multiple nests of C. iberica enables this species to abandon attacked nests and to reinstall their population in other nests of the same colony. This
reduces risk to the colonies because the route between the different nests is well known by transporter workers. 相似文献
53.
54.
55.
Cornille Fabrice Martin Loïc Lenoir Christine Cussac Didier Roques Bernard P. Fournié-Zaluski Marie-Claude 《International journal of peptide research and therapeutics》1997,4(4-6):207-212
Summary The light chain of tetanus neurotoxin (TeNTL chain) has been shown to be endowed with zine endopeptidase activity, selectively
directed towards the Gln76-Phe77 bond of synaptobrevin, a vesicle-associated membrane protein critically involved in neuroexocytosis. In previous reports,
truncations at the NH2- and COOH-terminus of synaptobrevin have shown that the sequence 39–88 of synaptobrevin is the minimum substrate of TeNT,
suggesting either the requirement of a well-defined three-dimensional structure of synaptobrevin or a role in the mechanism
of substrate hydrolysis for residues distal from the cleavage site. In this study, the addition of NH2- and COOH-terminal peptides of synaptobrevin, S 27–55 (S1) and S 82–93 (S2), to the synaptobrevin fragment S 56–81 allowed the cleavage of this latter peptide by TeNT to occur. This appears to result
from an activation process mediated by the simultaneous binding of S1 and S2 with complementary sites present on TeNT as shown by surface plasmon resonance experiments. All these results favor an exosite-controlled
hydrolysis of synaptobrevin by TeNT probably involving a conformational change of the toxin. This could accound for the high
degree of substrate specificity of TeNT and, probably, botulinum neurotoxins. 相似文献
56.
DNA binding properties of a chemically synthesized DNA binding domain of hRFX1. 总被引:2,自引:0,他引:2
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F Cornille P Emery W Schüler C Lenoir B Mach B P Roques W Reith 《Nucleic acids research》1998,26(9):2143-2149
The RFX DNA binding domain (DBD) is a novel highly conserved motif belonging to a large number of dimeric DNA binding proteins which have diverse regulatory functions in eukaryotic organisms, ranging from yeasts to human. To characterize this novel motif, solid phase synthesis of a 76mer polypeptide corresponding to the DBD of human hRFX1 (hRFX1/DBD), a prototypical member of the RFX family, has been optimized to yield large quantities (approximately 90 mg) of pure compound. Preliminary two-dimensional1H NMR experiments suggested the presence of helical regions in this sequence in agreement with previously reported secondary structure predictions. In gel mobility shift assays, this synthetic peptide was shown to bind in a cooperative manner the 23mer duplex oligodeoxynucleotide corresponding to the binding site of hRFX1, with a 2:1 stoichoimetry due to an inverse repeat present in the 23mer. The stoichiometry of this complex was reduced to 1:1 by decreasing the length of the DNA sequence to a 13mer oligonucleotide containing a single half-site. Surface plasmon resonance measurements were achieved using this 5'-biotylinated 13mer oligonucleotide immobilized on an avidin-coated sensor chip. Using this method an association constant (K a = 4 x 10(5)/M/s), a dissociation constant (K d = 6 x 10(-2)/s) and an equilibrium dissociation constant (K D = 153 nM) were determined for binding of hRFX1/DBD to the double-stranded 13mer oligonucleotide. In the presence of hRFX1/DBD the melting temperature of the 13mer DNA was increased by 16 degreesC, illustrating stabilization of the double-stranded conformation induced by the peptide. 相似文献
57.
58.
Cryptic Exons as a Source of Increased Diversity of Ewing Tumor-Associated EWS–FLI1 Chimeric Products 总被引:1,自引:0,他引:1
Heinrich Kovar Dragana Jugovic Thomas Melot Andreas Zoubek Gilbert Lenoir Franz-Martin Fink Irene Philip Claude Turc-Carel Gilles Thomas Jessica Zucman-Rossi 《Genomics》1999,60(3):371-374
In the Ewing family of tumors (EFT), the EWS gene is rearranged with members of the ets oncogene family. Variability in genomic breakpoint locations is the source of significant heterogeneity in fusion product structure. As a consequence of variably included exon sequences from the two partner genes, a variable amount of additional peptide sequence is inserted in between the minimal transforming domains. Some of this molecular diversity has recently been correlated with disparate clinical outcome. Here we report on cryptic exons found in the chimeric RNA of three EFT with different EWS-FLI1 fusions. In two tumors, the emergence of a cryptic exon from FLI1 intron 5 in the chimeric RNA was apparently unrelated to the genomic rearrangement that occurred in FLI1 introns 4 and 5, respectively. In one case, a novel exon was generated through the creation of an artificial splice acceptor site in FLI1 intron 6 by the genomic rearrangement that occurred in EWS intron 8. These results further extend the spectrum of molecular diversity in EFT. 相似文献
59.
C. Errard A.-M. Le Guisquet J.-P. Christidès J.-L. Mercier A. Lenoir A. Hefetz 《Insectes Sociaux》2008,55(2):115-122
Nestmate recognition in social insects generally involves matching a label to the template that is acquired through the early
learning of non-volatile cuticular hydrocarbon cues. However, a possible role of the volatile chemical cues that exist in
the nest, and which may also affect template formation, has not been studied. We investigated this possibility using experimental
mixedspecies groups composed of the two ant species Manica rubida and Formica selysi. The experimental set-up either allowed full contact between workers of the two species or interspecific contact was hindered
or prohibited by a single or a double mesh. After three months, workers of M. rubida ants were selected as focal ants for aggression tests including the following target ants: F. selysi workers from the same mixed-species group (for each of the three rearing conditions) or from a single-species group (control).
Workers of M. rubida were always amicable towards their group-mates, irrespective of the experimental group (contact, single or double mesh).
However, M. rubida that were not imprinted on F. selysi, expressed high levels of aggression towards the non-familiar F. selysi workers. The finding that F. selysi workers in the mixed-species groups appeared familiar to their M. rubida group-mates even without physical contact between them, suggests that the volatile cues produced by F. selysi affected nestmate recognition in M. rubida. In an attempt to identify these volatile cues we performed SPME analysis of the head space over groups of F. selysi workers. The findings revealed that F. selysi Dufour’s gland constituents, with undecane as the major product, are released into the head space, rendering them likely
candidates to affect template formation in M. rubida. Analysis of Dufour’s gland secretion of F. selysi revealed a series of volatile alkanes, including undecane as a major product. These alkanes were not present in the glandular
secretion of M. rubida, whose secretion was mainly composed of isomers of farnesene. We therefore hypothesize that callow M. rubida workers in the mixed-species groups had become imprinted by the above alkanes (in particular undecane, being the major heterospecific
volatile in the head space) and incorporated them into their own template.
Received 18 October 2007; revised 2 January 2008; accepted 7 January 2008. 相似文献
60.
L. Zinck D. Denis R. R. Hora C. Alaux A. Lenoir A. Hefetz P. Jaisson 《Journal of Insect Behavior》2009,22(5):362-374
In ants, queen adoption is a common way of achieving secondary polygyny but the mechanisms involved are little known. Here
we studied the process of long-term adoptions of alien queens in the facultative polygynous ant Ectatomma tuberculatum. In eight out of 10 successful adoption experiments, all the introduced queens showed similar behavior and fecundity as the
resident queens even after 2 months, indicating complete integration into the colony. Chemical analysis revealed that the
cuticular hydrocarbon profiles of resident and introduced queens were clearly distinct from those of workers and that they
did not change after adoption. We propose that queen-specific cuticular hydrocarbon profile may represent a reliable signal
of queen’s fertility and discuss about the evolution of high level of queen acceptance in E. tuberculatum. 相似文献