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排序方式: 共有812条查询结果,搜索用时 31 毫秒
811.
Lenka Halova David Cobley Mirita Franz-Wachtel Tingting Wang Kaitlin R. Morrison Karsten Krug Nicolas Nalpas Boris Ma
ek Iain M. Hagan Sean J. Humphrey Janni Petersen 《Open biology》2021,11(4)
Fluctuations in TOR, AMPK and MAP-kinase signalling maintain cellular homeostasis and coordinate growth and division with environmental context. We have applied quantitative, SILAC mass spectrometry to map TOR and nutrient-controlled signalling in the fission yeast Schizosaccharomyces pombe. Phosphorylation levels at more than 1000 sites were altered following nitrogen stress or Torin1 inhibition of the TORC1 and TORC2 networks that comprise TOR signalling. One hundred and thirty of these sites were regulated by both perturbations, and the majority of these (119) new targets have not previously been linked to either nutritional or TOR control in either yeasts or humans. Elimination of AMPK inhibition of TORC1, by removal of AMPKα (ssp2::ura4+), identified phosphosites where nitrogen stress-induced changes were independent of TOR control. Using a yeast strain with an ATP analogue-sensitized Cdc2 kinase, we excluded sites that were changed as an indirect consequence of mitotic control modulation by nitrogen stress or TOR signalling. Nutritional control of gene expression was reflected in multiple targets in RNA metabolism, while significant modulation of actin cytoskeletal components points to adaptations in morphogenesis and cell integrity networks. Reduced phosphorylation of the MAPKK Byr1, at a site whose human equivalent controls docking between MEK and ERK, prevented sexual differentiation when resources were sparse but not eliminated. 相似文献
812.
Sebela Marek; Luhov Lenka; Frbort Ivo; Hirota Shun; Faulhammer Heinz G.; Stuzka Vclav; Pec Pave 《Journal of experimental botany》1997,48(11):1897-1907
Amine oxidase from etiolated seedlings of fenugreek (Trigonellafoenumgraecum) has been isolated by a purification procedureinvolving three chromatographic steps. The homogeneousenzyme is of pink colour with a visible absorption maximum at500 nm. The dimeric enzyme (2 75 kDa) is a slightly acidicprotein (pl 6.8) containing 8% neutral sugars. Nterminalamino acid sequence of the enzyme shows a high degree of similarityto other plant and microbial coppercontaining amine oxidases.The best substrates of the enzyme are aliphatic diamines andsome polyamines, whereas inhibitors are substrate analogues,copper complexing agents, some alkaloids and several other compounds.Spectrophotometric titrations with phenylhydrazines demonstratedone reactive carbonyl group per subunit of the enzyme and redoxcyclicquinone staining after native electrophoresis indicatedthe presence of a quinone cofactor. Differential pulse polarographyshowed the existence of a copper/quinonecontaining activesite. The resonance Raman spectroscopy and the pHdependentshift of the absorption spectrum of the enzyme pnitrophenylhydrazoneconfirm unambiguously the identity of the cofactor with topaquinone. EPR spectra of the enzyme are in accordance with thoseof tetragonal cupric complexes as known for other coppercontainingamine oxidases. Besides the copper, Mn(II)ions were detectedthat partially occupy another metal site in the enzyme, buttheir catalytical importance is unlikely. Key words: Fenugreek, Trigonella foenumgraceum, amine oxidase, topa quinone 相似文献