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981.
Jill C. Preston Lena C. Hileman 《The Plant journal : for cell and molecular biology》2010,62(4):704-712
The degree to which developmental genetic pathways are conserved across distantly related organisms is a major question in biology. In Arabidopsis thaliana (L.) Heynh., inflorescence development is initiated in response to a combination of external and internal floral inductive signals that are perceived across the whole plant, but are integrated within the shoot apical meristem. Recently, it was demonstrated that SQUAMOSA‐PROMOTER BINDING PROTEIN (SBP)‐box proteins regulate A. thaliana flowering time by mediating signals from the autonomous and photoperiod pathways, and by directly activating key genes involved in inflorescence and floral meristem identity, including FRUITFULL (FUL), APETALA1 (AP1) and LEAFY (LFY). In the distantly related core eudicot species Antirrhinum majus L., paralogous SBP‐box proteins SBP1 and SBP2 have likewise been implicated in regulating the AP1 ortholog SQUAMOSA (SQUA). To test the hypothesis that SBP‐box genes are also involved in the floral induction of A. majus, we used a reverse genetic approach to silence SBP1. SBP1‐silenced lines are late to nonflowering, and show reduced apical dominance. Furthermore, expression and sequence analyses suggest that the SBP1‐mediated transition to flowering occurs through the positive regulation of FUL/LFY homologs. Together, these data outline the utility of virus‐induced gene silencing in A. majus, and provide new insight into the conservation of flowering time genetic pathways across core eudicots. 相似文献
982.
Sawada K Hasegawa M Tokuda L Kameyama J Kodama O Kohchi T Yoshida K Shinmyo A 《Bioscience, biotechnology, and biochemistry》2004,68(4):873-880
The rice Oryza sativa selenium-binding protein homologue (OsSBP) gene encodes a homologue of mammalian selenium-binding proteins, and it has been isolated as one of the genes induced by treating a plant with a cerebroside elicitor from rice blast fungus. The possible role of OsSBP in plant defense was evaluated by using a transgenic approach. Plants overexpressing OsSBP showed enhanced resistance to a virulent strain of rice blast fungus as well as to rice bacterial blight. The expression of defense-related genes and the accumulation of phytoalexin after infection by rice blast fungus were accelerated in the OsSBP overexpressors. A higher level of H(2)O(2) accumulation and reduced activity of such scavenging enzymes as ascorbate peroxidase and catalase were seen when the OsSBP-overexpressing plants were treated with the protein phosphatase 1 inhibitor, calyculin A. These results suggest that the upregulation of OsSBP expression conferred enhanced tolerance to different pathogens, possibly by increasing plant sensitivity to endogenous defense responses. Additionally, the OsSBP protein might have a role in modulating the defense mechanism to biotic stress in rice. 相似文献
983.
Asa Wilsson Sara Lind Lena Ohman Asa Nilsdotter-Augustinsson Helen Lundqvist-Setterud 《FEMS immunology and medical microbiology》2008,53(1):126-135
Staphylococcus epidermidis infections are usually nosocomial and involve colonization of biomaterials. The immune defense system cannot efficiently control the bacteria during these infections, which often results in protracted chronic inflammation, in which a key event is disturbed removal of neutrophils by tissue macrophages. While ingesting uninfected apoptotic neutrophils, macrophages release anti-inflammatory cytokines that lead to resolution of inflammation. In clinical studies, we have previously found elevated levels of the proinflammatory cytokines tumor necrosis factor-alpha (TNF-alpha) and interleukin-6 in synovial fluid from prostheses infected with coagulase negative staphylococci. We show that macrophages phagocytosing apoptotic neutrophils containing S. epidermidis released TNF-alpha and interleukin-6, whereas macrophages phagocytosing spontaneously apoptotic neutrophils did not. This difference was not due to dissimilar phagocytic capacities, because macrophages ingested both types of neutrophils to the same extent. The activation was induced mainly by the apoptotic neutrophils themselves, not by the few remaining extracellular bacteria. Macrophages were not activated by apoptotic neutrophils that contained paraformaldehyde-killed S. epidermidis. Proinflammatory reactions induced by clearance of apoptotic neutrophils containing S. epidermidis might represent an important mechanism to combat the infective agent. This activation of macrophages may contribute to the development of chronic inflammation instead of inflammation resolution. 相似文献
984.
A systems biology approach reveals major metabolic changes in the thermoacidophilic archaeon Sulfolobus solfataricus in response to the carbon source L‐fucose versus D‐glucose 下载免费PDF全文
Jacqueline Wolf Helge Stark Katharina Fafenrot Andreas Albersmeier Trong K. Pham Katrin B. Müller Benjamin H. Meyer Lena Hoffmann Lu Shen Stefan P. Albaum Theresa Kouril Kerstin Schmidt‐Hohagen Meina Neumann‐Schaal Christopher Bräsen Jörn Kalinowski Phillip C. Wright Sonja‐Verena Albers Dietmar Schomburg Bettina Siebers 《Molecular microbiology》2016,102(5):882-908
Archaea are characterised by a complex metabolism with many unique enzymes that differ from their bacterial and eukaryotic counterparts. The thermoacidophilic archaeon Sulfolobus solfataricus is known for its metabolic versatility and is able to utilize a great variety of different carbon sources. However, the underlying degradation pathways and their regulation are often unknown. In this work, the growth on different carbon sources was analysed, using an integrated systems biology approach. The comparison of growth on L‐fucose and D‐glucose allows first insights into the genome‐wide changes in response to the two carbon sources and revealed a new pathway for L‐fucose degradation in S. solfataricus. During growth on L‐fucose major changes in the central carbon metabolic network, as well as an increased activity of the glyoxylate bypass and the 3‐hydroxypropionate/4‐hydroxybutyrate cycle were observed. Within the newly discovered pathway for L‐fucose degradation the following key reactions were identified: (i) L‐fucose oxidation to L‐fuconate via a dehydrogenase, (ii) dehydration to 2‐keto‐3‐deoxy‐L‐fuconate via dehydratase, (iii) 2‐keto‐3‐deoxy‐L‐fuconate cleavage to pyruvate and L‐lactaldehyde via aldolase and (iv) L‐lactaldehyde conversion to L‐lactate via aldehyde dehydrogenase. This pathway as well as L‐fucose transport shows interesting overlaps to the D‐arabinose pathway, representing another example for pathway promiscuity in Sulfolobus species. 相似文献
985.
Background
The formylpeptide receptor family members FPR and FPRL1, expressed in myeloid phagocytes, belong to the G-protein coupled seven transmembrane receptor family (GPCRs). They share a high degree of sequence similarity, particularly in the cytoplasmic domains involved in intracellular signaling. The established model of cell activation through GPCRs states that the receptors isomerize from an inactive to an active state upon ligand binding, and this receptor transformation subsequently activates the signal transducing G-protein. Accordingly, the activation of human neutrophil FPR and FPRL1 induces identical, pertussis toxin-sensitive functional responses and a transient increase in intracellular calcium is followed by a secretory response leading to mobilization of receptors from intracellular stores, as well as a release of reactive oxygen metabolites. 相似文献986.
Changes in high-affinity uptake of choline (H.A.Ch) were studied in synaptosomes from different mouse brain regions following intravenous (i.v.) administration of atropine (0.3–30 mg/kg body weight) . The Ch-uptake was expressed as a Ch-uptake index, defined as the ratio between H.A.Ch and the corresponding choline acetylt-ransferase (ChAt) activity. The Ch-uptake index was highest in the hippocampus and lowest in the striatum. In the hippocampus a dose-dependent increase in this index was found following atropine treatment, while the striatal Ch-uptake index was unaffected by atropine. Atropine given i.v. in a dose of 10 mg/kg induced a 86% increase in Vmax in synaptosomes from the hippocampus. 相似文献
987.
988.
989.
Lena Sahlin Hong Wang Britt Masironi Arne Holmgren Hkan Eriksson 《The Journal of steroid biochemistry and molecular biology》1999,68(5-6):203-209
Estradiol has been shown to increase the level of thioredoxin mRNA in the uterus of the ovariectomized (ovx) rat. In this study the influence of progesterone, androgens, the anti-estrogen ICI 182780 and the anti-androgen Flutamid on thioredoxin expression, has been studied in the rat uterus. Thioredoxin mRNA concentrations were determined by solution hybridization. Ovx rats treated with progesterone alone showed no effect on thioredoxin expression. Combined treatment of ICI 182780 and estradiol attenuated the estradiol-induced increase in thioredoxin mRNA. When ovx rats were treated with a testosterone depot, the amount of thioredoxin mRNA was increased five-fold after 48 h and remained at that level during the rest of the 168 h monitored. A similar increase in thioredoxin mRNA could be seen after 5-dihydrotestosterone treatment, indicating a true androgenic effect. In addition, the anti-androgen Flutamid attenuated the thioredoxin mRNA increase seen after 5-dihydrotestosterone treatment alone.
It is concluded that thioredoxin mRNA is regulated by growth promoting gonadal steroids in the rat uterus. The attenuation of the estrogen and androgen-induced increases of the thioredoxin mRNA with ICI 182780 and Flutamid, indicate that the effect is mediated via the estrogen receptor and androgen receptor respectively. None of these hormones affected the hepatic thioredoxin mRNA level in the same animals. 相似文献
990.