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931.
Lena Struwe Peter E. Smouse Einar Heiberg Scott Haag Richard G. Lathrop 《Journal of Biogeography》2011,38(10):1841-1854
Aim Spatial evolutionary and ecological vicariance analysis (SEEVA) is a simple analytical method that evaluates environmental or ecological divergence associated with evolutionary splits. It integrates evolutionary hypotheses, phylogenetic data, and spatial, temporal, environmental and geographical information to elucidate patterns. Using a phylogeny of Prepusa Mart. and Senaea Taub. (Angiospermae: Gentianaceae), SEEVA is used to describe the radiation and ecological patterns of this basal gentian group across south‐eastern Brazil. Location Latin America, global. Methods Environmental data for 151 geolocated botanical collections, associated with specimens from seven species, were compiled with Arc GIS, and were matched with geolocated base layers of eight climatological variables, as well as one each of geological, soil type, elevational and vegetation variables. Sister groups were defined on the basis of the six nested nodes that defined the phylogenetic tree of these two genera. A (0, 1)‐scaled divergence index (D) was defined and tested for each of 12 environmental and for each of the six phylogenetic nodes, by means of contingency analyses. We contrast divergence indices of nested clades, allopatric and sympatric sister clades. Results The level of ecological divergence between sister clades/species, defined in terms of D measures, was substantial for five of six nodes, with 21 of 72 environmental comparisons having D > 0.75. Soil types and geological age of bedrock were strongly divergent only for basal nodes in the phylogeny, by contrast with temperature and precipitation, which exhibited strong divergence at all nodes. There has been strong divergence and progressive occupation of wetter and colder habitats throughout the history of Prepusa. Nodes separating allopatric sister clades exhibited larger niche divergence than did those separating sympatric sister clades. Main conclusions SEEVA provides a multi‐source, direct analysis method for correlating field collections, phylogenetic hypotheses, species distributions and georeferenced environmental data. Using SEEVA, it was possible to quantify and test the divergence between sister lineages, illustrating both niche conservatism and ecological specialization. SEEVA permits elucidation of historical and ecological vicariance for evolutionary lineages, and is amenable to wide application, taxonomically, geographically and ecologically. 相似文献
932.
利用固相微萃取技术(SPME)偶联气相色谱-质谱(GC-MS)分析燕山山脉一种香料型乳菇——香亚环乳菇Lactarius subzonarius的挥发性成分。共检出25种挥发性成分,其中氧杂环化合物3种、醛类1种、酯类13种、烯烃类3种、芳香族化合物2种和烷烃类3种。具有葫芦巴感官气味的挥发性组分3-羟基-4,5-二甲基-2(5H)-呋喃酮的相对含量35.76%,该成分是其近缘种Lactarius helvus的主要香味化合物,故推测3-羟基-4,5-二甲基-2(5H)-呋喃酮是其关键香味成分。 相似文献
933.
月季F1代群体表型性状变异分析 总被引:1,自引:0,他引:1
以月季(Rosa spp.)品种‘云蒸霞蔚’和‘太阳城’正交获得的184株F1代群体为研究对象,采用方差分析、变异系数、遗传分析、相关性分析对花部形态性状以及叶片形态性状进行测定分析。结果表明:该杂交群体表型变异丰富,变异系数在7.33%~68.08%,其中花瓣数量在群体中的变异程度最高;杂交后代各个性状上均发生分离变异,出现不同于亲本的表现型,且离散程度较高。表型性状的遗传特点与关联分析,为挖掘控制表型性状的优良基因及辅助选择育种提供参考。 相似文献
934.
Erwan Lagadec Yann Gomard Gildas Le Minter Colette Cordonin Eric Cardinale Beza Ramasindrazana Muriel Dietrich Steven M Goodman Pablo Tortosa Koussay Dellagi 《PLoS neglected tropical diseases》2016,10(8)
Leptospirosis is a bacterial zoonosis of major concern on tropical islands. Human populations on western Indian Ocean islands are strongly affected by the disease although each archipelago shows contrasting epidemiology. For instance, Mayotte, part of the Comoros Archipelago, differs from the other neighbouring islands by a high diversity of Leptospira species infecting humans that includes Leptospira mayottensis, a species thought to be unique to this island. Using bacterial culture, molecular detection and typing, the present study explored the wild and domestic local mammalian fauna for renal carriage of leptospires and addressed the genetic relationships of the infecting strains with local isolates obtained from acute human cases and with Leptospira strains hosted by mammal species endemic to nearby Madagascar. Tenrec (Tenrec ecaudatus, Family Tenrecidae), a terrestrial mammal introduced from Madagascar, is identified as a reservoir of L. mayottensis. All isolated L. mayottensis sequence types form a monophyletic clade that includes Leptospira strains infecting humans and tenrecs on Mayotte, as well as two other Malagasy endemic tenrecid species of the genus Microgale. The lower diversity of L. mayottensis in tenrecs from Mayotte, compared to that occurring in Madagascar, suggests that L. mayottensis has indeed a Malagasy origin. This study also showed that introduced rats (Rattus rattus) and dogs are probably the main reservoirs of Leptospira borgpetersenii and Leptospira kirschneri, both bacteria being prevalent in local clinical cases. Data emphasize the epidemiological link between the two neighbouring islands and the role of introduced small mammals in shaping the local epidemiology of leptospirosis. 相似文献
935.
Guillaume Gaullier Simona Miron Sabrina Pisano Rémi Buisson Yann-Va? Le?Bihan Carine Tellier-Lebègue Wala Messaoud Pierre Roblin Beatriz G. Guimar?es Robert Thai Marie-Josèphe Giraud-Panis Eric Gilson Marie-Hélène Le?Du 《Nucleic acids research》2016,44(4):1962-1976
Telomere integrity is essential to maintain genome stability, and telomeric dysfunctions are associated with cancer and aging pathologies. In human, the shelterin complex binds TTAGGG DNA repeats and provides capping to chromosome ends. Within shelterin, RAP1 is recruited through its interaction with TRF2, and TRF2 is required for telomere protection through a network of nucleic acid and protein interactions. RAP1 is one of the most conserved shelterin proteins although one unresolved question is how its interaction may influence TRF2 properties and regulate its capacity to bind multiple proteins. Through a combination of biochemical, biophysical and structural approaches, we unveiled a unique mode of assembly between RAP1 and TRF2. The complete interaction scheme between the full-length proteins involves a complex biphasic interaction of RAP1 that directly affects the binding properties of the assembly. These results reveal how a non-DNA binding protein can influence the properties of a DNA-binding partner by mutual conformational adjustments. 相似文献
936.
Host specific variation in photosynthesis of an obligate xylem‐tapping mistletoe Dendrophthoe curvata in a Bornean heath forest 下载免费PDF全文
Quang‐Vuong Le Kushan U. Tennakoon Faizah Metali Linda B. L. Lim Jay F. Bolin 《Nordic Journal of Botany》2016,34(2):235-243
Most mistletoe–host ecophysiological studies have paid attention to the influence of parasites on host performance. This paper explored the impact of varying hosts on the photosynthesis of a single mistletoe species. Here, we studied an obligate xylem‐tapping tropical mistletoe (Dendrophthoe curvata (Blume) Miquel) parasitizing four different hosts (Acacia auriculiformis A. Cunn. Ex Benth, Andira inermis (W. Wright) DC., Mangifera indica L. and Vitex pinnata L.) in a homo geneous tropical heath forest patch in Brunei Darussalam. We compared photosynthetic capacity and photosynthesis‐related characteristics of the mistletoe on four different hosts to evaluate the overall impact of hosts on the parasite. Results showed that the mistletoe–host patterns of CO2 assimilation rates, transpiration rates and water use efficiency varied significantly based on the host. In the D. curvata–Vitex pinnata association, the mistletoe exhibited significantly lower CO2 assimilation rates but showed no significant variations in transpiration rates and water use efficiency when compared to the host. In D. curvata–Andira inermis and D. curvata–Mangifera indica associations, the mistletoe showed significantly higher photosynthetic rates than the hosts, whereas in the D. curvata–Acacia auriculiformis association, there was no significant difference in photosynthetic rates between the counterparts. Host specificity also significantly influenced some mistletoe photosynthetic parameters such as light saturated photosynthesis, specific leaf area, leaf chlorophyll content, CO2 assimilation rates, stomatal conductance, transpiration rates and water use efficiency. Different tree hosts intrinsically offer different resources to their obligate mistletoe parasites based on their physiology and environmental parameters. We argue that host‐specific responses have driven these intra‐specific variations in mistletoe physiology. This study provides background for future investigation on potential host‐regulated mechanisms that drive functional changes in host‐dependent mistletoes. 相似文献
937.
Invasion history and demographic processes associated with rapid morphological changes in the Red‐whiskered bulbul established on tropical islands 下载免费PDF全文
Ariane Le Gros Philippe Clergeau Dario Zuccon Raphaël Cornette Blake Mathys Sarah Samadi 《Molecular ecology》2016,25(21):5359-5376
The Red‐whiskered bulbul is a very successful invasive bird species. Morphological differences have been reported among individuals inhabiting the humid and dry coasts of Reunion Island, in a 30‐year‐old population. This suggests a capacity for rapid local adaptation which could explain the general invasive success of this species. However, the origin and invasion history of this population is unknown. It is therefore not possible to establish with certainty the cause of these morphological differences. Here, we investigated the invasion history of populations of Red‐whiskered bulbul established on Reunion Island, Mauritius and Oahu (three geographically similar tropical islands) to assess the link between invasion history and morphological changes in these populations. We first assessed the source(s) of the invasive populations. We then compared the morphology of the individuals between the invasive and native populations and between the dry and humid coasts of invaded islands. Finally, we inferred the invasion history of the invasive populations to investigate the role of neutral processes (e.g. founder effect and drift) on morphology. We found that the invasive populations have a similar origin and that the morphology of the individuals in these populations has diverged in a similar way from the native range, suggesting a convergent adaptation to tropical islands. Like on Reunion, we found differences in morphology between the dry and humid coasts on Mauritius. These morphological differences can be explained by invasion history on Reunion but not on Mauritius. Both neutral evolution and adaptation thus shape the morphology of invasive Red‐whiskered bulbuls. 相似文献
938.
Influence of colostrum intake on piglet survival and immunity 总被引:1,自引:0,他引:1
Devillers N Le Dividich J Prunier A 《Animal : an international journal of animal bioscience》2011,5(10):1605-1612
Colostrum intake from birth to 24 h after the onset of parturition (T24) was estimated for 526 piglets from 40 litters. Plasma concentrations of immunoglobulin G (IgG), lactate, glucose and cortisol were determined at T24 for six piglets per litter. Plasma IgG concentration was also assayed at weaning (28 days) on the same piglets. Rectal temperature was measured at T24 on all piglets. Mortality was recorded until weaning and comparisons were made between piglets that died before weaning and those that were still alive at weaning. The piglets that died before weaning had lower birth weight, lower colostrum intake, lower weight gain between birth and T24, and had a lower rectal temperature, higher plasma cortisol concentration and lower plasma IgG and glucose concentrations at T24 than piglets still alive at weaning. In addition, a higher proportion of piglets that died before weaning had difficulty taking their first breath after birth and were affected by splayleg. Considering all piglets, colostrum intake was positively related to rectal temperature and plasma glucose concentration and negatively related to plasma cortisol concentration at T24. Plasma IgG concentration at T24 was explained by colostrum intake, IgG concentration in the ingested colostrum, birth weight and birth rank (P<0.0001). Plasma IgG concentration at weaning was related to plasma IgG concentration at T24 (r=0.54; P<0.0001) and to colostrum intake (r=0.32; P<0.0001). Finally, body weight was explained by colostrum intake, birth weight and age until 6 weeks of age (P<0.0001). These results show that colostrum intake is the main determinant of piglet survival through provision of energy and immune protection and has potential long-term effects on piglet growth and immunity. 相似文献
939.
Guiet R Van Goethem E Cougoule C Balor S Valette A Al Saati T Lowell CA Le Cabec V Maridonneau-Parini I 《Journal of immunology (Baltimore, Md. : 1950)》2011,187(7):3806-3814
Tumor-associated macrophages are known to amplify the malignant potential of tumors by secreting a variety of cytokines and proteases involved in tumor cell invasion and metastasis, but how these macrophages infiltrate tumors and whether the macrophage migration process facilitates tumor cell invasion remain poorly documented. To address these questions, we used cell spheroids of breast carcinoma SUM159PT cells as an in vitro model of solid tumors. We found that macrophages used both the mesenchymal mode requiring matrix metalloproteinases (MMPs) and the amoeboid migration mode to infiltrate tumor cell spheroids. Whereas individual SUM159PT cells invaded Matrigel using an MMP-dependent mesenchymal mode, when they were grown as spheroids, tumor cells were unable to invade the Matrigel surrounding spheroids. When spheroids were infiltrated or in contact with macrophages, tumor cell invasiveness was restored. It was dependent on the capacity of macrophages to remodel the matrix and migrate in an MMP-independent mesenchymal mode. This effect of macrophages was much reduced when spheroids were infiltrated by Matrigel migration-defective Hck(-/-) macrophages. In the presence of macrophages, SUM159PT migrated into Matrigel in the proximity of macrophages and switched from an MMP-dependent mesenchymal migration to an amoeboid mode resistant to protease inhibitors.Thus, in addition to the well-described paracrine loop between macrophages and tumor cells, macrophages can also contribute to the invasiveness of tumor cells by remodeling the extracellular matrix and by opening the way to exit the tumor and colonize the surrounding tissues in an MMP-dispensable manner. 相似文献
940.
Sarfo FS Le Chevalier F Aka N Phillips RO Amoako Y Boneca IG Lenormand P Dosso M Wansbrough-Jones M Veyron-Churlet R Guenin-Macé L Demangel C 《PLoS neglected tropical diseases》2011,5(7):e1237