免疫刺激复合物疫苗制备及其对小鼠免疫功能的影响

确定了免疫刺激复合物(ISCOM)疫苗的安全有效剂量及其对小鼠免疫功能的影响。选取体重25 g左右的昆明小白鼠60只,分为12组,每组5只,腹腔分别注射不同剂量(5-200μg)的ISCOM疫苗,观察小鼠健康状态。选取体重28 g左右的昆明小白鼠60只,分为4组,分别在腹腔注射相同剂量的灭菌生理盐水,Lipase+生理盐水,空ISCOM,Lipase+ISCOM疫苗,利用间接ELISA检测血清中特异性抗体效价。结果表明,当注射剂量为5-25μg/只时,小鼠无任何异常症状。免疫后8 d,小鼠血清特异性抗体效价(log2)可以达到10.5,显著高于对照组。因此,ISCOM疫苗能有效引起小鼠的免疫反应。     

科尔沁沙地盐蒿种群的空间分布格局及关联性

以科尔沁沙地半固定沙丘和流动沙丘两种不同沙丘上的盐蒿(Artemisia halodendron)种群为研究对象,采用空间点格局方法研究了 0～20 m尺度上盐蒿种群的空间分布格局及其关联性.结果表明,半固定沙丘上盐蒿种群的数量远大于流动沙丘,不同生长发育阶段的种群结构呈偏正态分布,属于稳定型种群;流动沙丘上不同生长发...     

某冶炼厂周围8种植物对重金属的吸收与富集作用

采用野外采样系统分析法,研究了沈阳冶炼厂的8种植物对重金属(Pb、Zn、Cu和Cd)的吸收与富集作用以及可能的耐性机制.结果表明,植物对重金属的吸收和富集,因植物种类、部位及重金属种类而不同.茼麻(Abutilon theophrasti)对Pb的吸收和富集能力较强,小白酒花(Conyza canadensis)、三裂叶豚草(Ambrosia trifida)、酸模叶蓼(Polygonum lapathifolium)、茼麻、龙葵(Solanum nigrum)、绿珠藜(Chenopodium acuminatum)和菊芋(Helianthus tuberosus)对Zn的吸收和富集效果较好,绿珠藜和茼麻对Cu的吸收和富集能力较强,龙葵、绿珠藜、茼麻、酸模叶蓼和小白酒花对Cd的吸收和富集能力较强.这些植物向地上部转移某些重金属的能力很强,转移系数大于1,可用于植物提取方式的污染土壤修复.其他转移系数小于1的植物,适合于重金属污染土壤的植物稳定.     

苦豆子提取物与埃玛菌素混用对小菜蛾生长发育和繁殖的影响

将苦豆子甲醇和水提取物分别与埃玛菌素混合,在室内测定了两混合物对小菜蛾生长发育和繁殖的影响.结果表明:苦豆子甲醇提取物 埃玛菌素和苦豆子水提取物 埃玛菌素对小菜蛾3龄幼虫口服毒力明显高于埃玛菌素,其LC50值分别为0.19、0.32和0.51 mg.L-1;用两混合物以及埃玛菌素亚致死剂量处理3龄幼虫,试虫化蛹率、羽化率和成虫的繁殖力均呈现明显不良影响.     

DHPLC检测胃癌微卫星不稳定性

为探讨一种快速、简便、可靠的胃癌微卫星不稳定性（MSI）检测方法,变性聚丙烯酰胺凝胶电泳-银染法检测28例胃癌12个微卫星位点(D1S548、D1S552、D5S346、TP53、IGFIIR(G)8、IGFIIR(CT)5、TGFßRII(GT)3、TGFßRII(A)10、hMSH3(A)8、hMSH6(G)8、BAX(G)8和Bat26）,DHPLC柱温50℃检测Bat26位点。凝胶电泳发现MSI-H 2例（7.14%）,MSI-L胃癌15例（53.6%）,Bat26+2例均为MSI-H,Bat26改变和MSI-H表型一致（P<0.01,Fisher’s确切概率法）。DHPLC亦证实2例Bat26+胃癌,结果和凝胶电泳完全一致。结果表明,DHPLC检测Bat26位点是研究胃癌MSI-H的较好方法,有一定的临床应用价值。Abstact: To establish a fast, simple and solid method of studying microsatellite instability (MSI) in gastric cancer, a panel of 12 microsatellite sites,D1S548, D1S552, D5S346, TP53, IGFIIR(G)8, IGFIIR(CT)5, TGFßRII(GT)3, TGFßRII(A)10, hMSH3(A)8, hMSH6(G)8, BAX(G)8 and Bat26, were detected by denatured polyacrymide gel electrophoresis-silver stain in 28 gastric cancers. Bat26 was also analyzed by denatured high performance liquid chromatograph (DHPLC) at 50℃ in the DNASep Cartridge. Two MSI-H (7.14%) and 15 MSI-L cancers (53.6%) were identified in 28 gastric cancers. Bat26 was positive only in 2 MSI-H cancers. The alterations of Bat26 and MSI-H status were coincident (P<0.01). The two Bat26+ cancers were also confirmed by DHPLC. Results obtained from DHPLC and gel electrophoresis were completely consistent. Thus, DHPLC analysis of Bat26 site may be a favorable method of detecting MSI-H status in gastric cancer, and be of clinical importance.     

力学环境对软骨基质代谢的影响

正常关节软骨所受压力是由动态压力与静态压力交替完成。压力引起软骨一系列生理变化包括细胞及细胞外基质成分变形、组织内液体流动、水流电位和生理生化变化。这些变化直接调控细胞外基质代谢。体外构建有良好功能的组织工程化软骨是目前软骨病变、缺损理想的修复方法。研究力学环境对软骨基质代谢的影响,对构建组织工程化软骨有深远意义。     

基于动力学模型的法夫酵母发酵生产虾青素的补料策略优化

对法夫酵母的不同补料发酵方式进行了研究.基于底物抑制模型,提出了一种优化的两阶段补料策略,用于法夫酵母产虾青素的高密度发酵.在发酵的延迟期和对数生长期早期,糖浓度控制在25 g/L左右,在此条件下,生物量可以达到最大,且时间缩短.在对数生长期后期及稳定期,糖浓度控制在5 g/L,虾青素的合成时间可以有效延长.与传统的补料方式相比,采用此补料策略取得了较好的发酵效果.发酵终点细胞干重达到23.8g/L,虾青素产量达到29.05 mg/L,分别比分批发酵提高了52.8%和109%.     

Specific endotoxic lipopolysaccharide-binding receptors on murine splenocytes. III. Binding specificity and characterization.

In previously published studies, we employed a photoreactive radioiodinated derivative of LPS from Escherichia coli 0111:B4 to identify and characterize a membrane-localized specific LPS binding protein of approximately 80-kDa molecular mass. Our more recent studies demonstrating that mAb with specificity for this 80-kDa protein will act as an agonist in mediating macrophage activation have established that this protein serves as a specific receptor for LPS. In the experiments reported here, we have more accurately determined the apparent molecular mass of this protein to be 73 kDa (p73). We have also extended the sources of LPS-derivatized photo-cross-linking preparations (including Re-LPS) to determine generality of LPS binding to this receptor. Binding to the p73 LPS receptor is demonstrated with all of the LPS derivatives synthesized in our laboratory, as well as probes synthesized by other investigators. Binding of S-LPS is readily inhibited by Re chemotype LPS, and we have shown that this competitive inhibition is most likely not the result of formation of LPS aggregates. These results confirm and extend our earlier studies suggesting that the binding of LPS to the p73 receptor is lipid A specific. We further demonstrate that, in contrast to results published in a recent report, the p73 LPS receptor has no significant binding specificity for a variety peptidoglycan polymer preparations. Finally, we show that this LPS receptor can be detected on murine fibroblast, macrophage, and mastocytoma cell lines. Differences have been observed in the level of expression of LPS receptors on the various cell lines studied.     

江苏省区域农业生态经济的时空变异分析

以江苏省徐连、沿海、宁镇扬、沿江和太湖5个经济区及其9个代表县市区域为研究对象,基于"社会-生态-经济"的系统分析,建立了包括目标层、准则层和指标层3个层次多指标的农业生态经济综合评价指标体系,采用主成分分析方法对评价指标体系进行检验、筛选,并以江苏省为案例,综合分析了农业生态经济系统的时空变异特征。结果表明:江苏省农业生态经济发展水平的时空变异明显,空间上,徐连、沿海、宁镇扬、沿江和太湖5个经济区表现出由北向南逐渐上升的趋势,且随时间的推移逐渐上升;时间上,徐连和太湖经济区农业生态经济发展水平在1999—2008年间呈持续上升,处于良好或优质协调状态。虽然两经济区的农业生态环境-经济发展协调发展水平均较高,但差距较大,徐连和太湖经济区的农业生态环境-经济发展协调度从1999年到2008年呈逐渐下降趋势,但总体上仍处于良好或优质协调状态。