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991.
A chalcone synthase-like cDNA from rice anther 总被引:3,自引:0,他引:3
992.
L. Ferretti B. G. D. Urquhart A. Eggen I. Olsaker B. Harlizius B. Castiglioni A. Mezzelani S. Solinas Toldo U. Thieven Y. Zhang A. L. G. Morgan V. M. Teres M. Schwerin I. Martin-Burriel B. P. Chowdhary G. Erhardt I. J. Nijman E. P. Cribiu W. Barendse H. Leveziel R. Fries J. L. Williams 《Mammalian genome》1997,8(1):29-36
The mapping strategy for the bovine genome described in this paper uses large insert clones as a tool for physical mapping
and as a source of highly polymorphic microsatellites for genetic typing, and was one objective of the BovMap Project funded
by the European Union (UE). Eight-three cosmid and phage clones were characterized and used to physically anchor the linkage
groups defining all the bovine autosomes and the X Chromosome (Chr). By combining physical and genetic mapping, clones described
in this paper have led to the identification of the linkage groups corresponding to Chr 9, 12, 16, and 25. In addition, anchored
loci from this study were used to orient the linkage groups corresponding to Chr 3, 7, 8, 9, 13, 16, 18, 19, and 28 as identified
in previously published maps. Comparison of the estimated size of the physical and linkage maps suggests that the genetic
length of the bovine genome may be around 4000 cM.
Received: 1 July 1996 / Accepted: 13 September 1996 相似文献
993.
In this study we describe the contribution of matrilineal and patrilineal effects on the adiposity, body weight, and on the
weights of individual fat pads in F2 male mice derived from an SWR/J × AKR/J cross. AKR/J mice become obese after 12 weeks on a high-fat diet, whereas SWR/J mice
remain relatively lean. Here we report that mice with AKR maternal and AKR paternal grandmothers have significantly larger
epidydimal and retroperitoneal fat pads than those with SWR maternal and paternal grandmothers. However, grandparental strain
had no effect on the overall adiposity (AI) or the weights of the inguinal, subcutaneous or mesenteric fat pads. The strain
of the paternal grandparents had a small but significant effect on body weight. These effects can be attributed to in utero
effects, imprinting effects, cytoplasmic and/or Y chromosome transmission of factors controlling body fat. We also describe
the presence of a quantitative trait locus (QTL) on Chromosome X, close to DXMit174, which is linked to adiposity, body weight, and to the weights of the individual fat depots. However, this QTL is not responsible
for the grandparental strain effects described above.
Received: 3 March 1997 / Accepted: 5 May 1997 相似文献
994.
Zhang Ying; Iwasa Tatsuo; Tsuda Motoyuki; Kobata Akira; Takasaki Seiichi 《Glycobiology》1997,7(8):1153-1158
The N-linked sugar chains were liberated as oligosaccha-ridesfrom octopus rhodopsin by hydrazinolysis. Most of the oligosaccharideswere neutral, and separated into two major components by columnchromatography using immobilized lectins and Bio-Gel P-4. Structuralanalysis of the one major component by sequential exoglycosidasedigestion, chemical fragmentation in combination with meth-ylationanalysis revealed that it is a nonasaccharide; Man16(Gaiβ13GlcNAcβ12Man13)Manβ14GlcNAcβ14(Galβ14Fuc16)GlcNAcThis structure is quite unique in that a novel galactosylatedfucose residue is attached to the reducing terminal N-acetyl-glucosamineresidue. galactosylated Fuc N-linked sugar chain novel structure octopus rhodopsin 相似文献
995.
Ming Li Wang Lu Huang Deverie K. Bongard-Pierce Stephen Belmonte Eve Ann Zachgo John W. Morris Maureen Dolan Howard M. Goodman 《The Plant journal : for cell and molecular biology》1997,12(3):711-730
A method for construction of bacterial artificial chromosome (BAC) contigs from a yeast artifical chromosome (YAC) physical map is described. An ∼2 Mb contig, consisting of two large BAC contigs linked by a small YAC, has been assembled in the region around 80 cM of Arabidopsis thaliana chromosome 2. Clones from this contig will facilitate gene isolation in the region and can be used directly as substrates for DNA sequencing. 相似文献
996.
西藏南迦巴瓦峰地区孟加拉虎的初步研究 总被引:4,自引:2,他引:2
1994年5~6月、9~10月及1995年6~12月,作者对西藏东南部南迦巴瓦峰和邻近地区的孟加拉虎的分布及生态进行了野外调查,初步弄清了该地区虎的现状。通过在墨脱县格当乡的抽样调查及跟踪观察虎的移动和觅食,查明了金珠藏布江流域内近两年有关虎捕食大型牲畜的报告属实。1993年10月至1995年7月,该河谷内因虎捕食共损失牛、马和骡302头,估计活体总重量约59881kg,平均月损失2722kg,相当于自然状态下11头虎的月进食量。全乡12个村的牲畜均受到不同程度的影响,受损的户数占该乡总户数的62%,户牲畜损失率为7.7%~100%不等。对其它动物的调查表明,虎大量捕食牲畜与低海拔河谷的开发和环境中虎的自然食物缺乏有关 相似文献
997.
原生质体电融合酵母多倍体生理特性的研究 总被引:5,自引:0,他引:5
对原生质体电融合技术获得的11株酵母多倍体融合株,进行了一系列生理特性的分析比较.结果发现,在电融合过程中,融合株的细胞体积及DNA含量并不随着核倍性呈线性递增.而是有其特殊的变化规律。当糖化酵母sta1、sta2和sta3在细胞核中各自纯合时,明显表现出表达的剂量效应。其中sta1、sta2纯合的融合株.在YEPS培养中,GA分泌可被淀粉大量诱导,表现出一定的二次生长特性。从分析结果推测,在亲株5301-14D及HU-TY—1A中可能有对GA分泌不利的因素。 相似文献
998.
Recent physiological and pharmacological studies have indicated the potential importance of tryptase, the major protein component in mast cells, in inflammatory diseases (especially asthma). Being released at inflammatory sites after the activation of mast cells, tryptase is capable of causing bronchohyperresponsiveness and infiltration of eosinophils, neutrophils, etc. in animal airways. The mechanisms by which tryptase causes bronchoconstriction involve probably the potentiation of other chemical mediators such as histamine, production of bradykinin via the hydrolysis of kininogen, and cleavage of the bronchodilating peptides VIP (vasoactive intestinal peptide) and PHM (peptide histidine-methionine). Tryptase has also been found to be a potent mitogen in vitro for airway smooth muscle cells and epithelial cells, implying its role in the hyperplasia of the asthmatic airways. The experimental data providing evidence for the above roles of tryptase are summarized in the present review, as well as the effects of tryptase inhibition in animal asthma models. The potential strategies for the development of anti-asthmatic agents based on the inhibition of tryptase are discussed. 相似文献
999.
本文对观赏植物异色芋(ColocasiaheterochromaH.LietZ.X.Wei)在昆明栽培环境下的生物学特性进行总结,为商品化栽培异色芋提供了理论依据,为其分类也提供了一定的有益补充。结果表明:一年生植株不开花,根茎也不产生分枝;二年生植株开花率为73.6%,根茎平均分枝3.6条。异色芋3月上旬开始萌动,7月中旬抽花梗,8月上旬开花,11月份地上部分枯萎,因此在昆明异色芋生育期约260d。 相似文献
1000.
Spectroscopic techniques were used to investigate the interaction between vanadate and human erythrocyte ghosts. Direct evidence from 51V nuclear magnetic resonance (NMR) studies suggested that the monomeric and polymeric vanadate species may bind to the anion binding sites of band 3 protein of the erythrocyte membrane. The results of 51V NMR studies and the quenching effect of vanadate on the intrinsic fluorescence of the membrane proteins indicated that in the low concentration range of vanadate (<0.6 mm), monomeric vanadate binds mostly to the anion sites of band 3 protein with the dissociation constant close to 0.23 mm. The experiments of sulfhydryl content titration by the method of Ellman and residue sulfhydryl-labeled fluorescence spectroscopies clearly displayed that vanadate reacts directly with sulfhydryl groups. The appearance of the anisotropic election spin resonance (ESR) signal of vanadyl suggests that a small (c. 3%) amount of vanadate was reduced by sulfhydryl groups of membrane proteins. The fluidity and order of intact ghost membrane were reduced by the reaction with vanadate, as shown by the ESR studies employing the protein- and lipid-specific spin labels. It was concluded that although vanadates mainly bind to band 3 protein, a minor part of vanadate may oxidize the residue sulfhydryl groups of membrane proteins, and thus decrease the fluidity of erythrocyte membrane. 相似文献