Direct Observation of Membrane Insertion by Enveloped Virus Matrix Proteins by Phosphate Displacement

Enveloped virus release is driven by poorly understood proteins that are functional analogs of the coat protein assemblies that mediate intracellular vesicle trafficking. We used differential electron density mapping to detect membrane integration by membrane-bending proteins from five virus families. This demonstrates that virus matrix proteins replace an unexpectedly large portion of the lipid content of the inner membrane face, a generalized feature likely to play a role in reshaping cellular membranes.     

The Wilms' tumor suppressor Wt1 is associated with the differentiation of retinoblastoma cells.

We have demonstrated recently that Wilms' tumor suppressor 1 (Wt1),in addition to its role in genitourinary formation,is required for the differentiation of ganglion cells in the developing retina. Here we provide further evidence that Wt1 is associated with neuronal differentiation. Thus, the retinoblastoma-derived human cell line, Y-79, contained robust amounts of Wt1 mRNA and protein. Wt1 expression was down-regulated upon laminin-induced differentiation of Y-79 into neuron-like cells. Inhibition of Wt1 with antisense oligonucleotides dramatically reduced the capacity of undifferentiated Y-79 cells to undergo neuronal differentiation, whereas sense and missense oligonucleotides had no effect. Wt1 immunoreactivity was also detected in solid retinoblastomas, in which it resided mainly in areas with moderate proliferative activity. These findings suggest a role for Wt1 in the differentiation of retinoblastoma cells. Furthermore, Wt1 expression in retinoblastoma may reflect the potential of these tumors to initiate the early steps of neuronal differentiation.     

Patterns of crop damage by wild boar (Sus scrofa)in Luxembourg over a 10-year period

In many European countries, the wild boar (Sus scrofa) is often associated with crop damage. In this study, we analyse data relating to 13,276 cases of wild boar damage to agricultural crops over a 10-year period in Luxembourg (an area of 2,586 km² in Western Europe). Results show that (1) damage is more severe in this area than in others; (2) damage to permanent grassland is far more frequent and more severe than damage to annual crops; (3) trichomatous crops such as barley are avoided; (4) damage is seasonally distributed according to type of crop; (5) damage is distributed spatially in a non-uniform manner; (6) damage intensity is significantly correlated with wild boar hunting bags, both over time and space. We suggest that wild boar management strategy should always take into account the issue of damage to agricultural crops. Our results imply that measures for preventing or reducing damage should be more targeted in time and space and that adjustments to cropping patterns should contribute towards a reduction of wild boar damage.     

Modelling Foot-and-Mouth Disease Virus Dynamics in Oral Epithelium to Help Identify the Determinants of Lysis

Foot-and-mouth disease virus (FMDV) causes an economically important disease of cloven-hoofed livestock; of interest here is the difference in lytic behaviour that is observed in bovine epithelium. On the skin around the feet and tongue, the virus rapidly replicates, killing cells, and resulting in growing lesions, before eventually being cleared by the immune response. In contrast, there is usually minimal lysis in the soft palate, but virus may persist in tissue long after the animal has recovered from the disease. Persistence of virus has important implications for disease control, while identifying the determinant of lysis in epithelium is potentially important for the development of prophylactics. To help identify which of the differences between oral and pharyngeal epithelium are responsible for such dramatically divergent FMDV dynamics, a simple model has been developed, in which virus concentration is made explicit to allow the lytic behaviour of cells to be fully considered. Results suggest that localised structuring of what are fundamentally similar cells can induce a bifurcation in the behaviour of the system, explicitly whether infection can be sustained or results in mutual extinction, although parameter estimates indicate that more complex factors may be involved in maintaining viral persistence, or that there are as yet unquantified differences between the intrinsic properties of cells in these regions.     

Stone martens (<Emphasis Type="Italic">Martes foina</Emphasis>) and cars: investigation of a common human–wildlife conflict

Stone martens (Martes foina) commonly climb into car engine compartments, where they may tear up heat and noise insulation mats or bite into rubber or plastic components. This brings martens into conflict with humans. Although various hypotheses have been suggested as to why martens damage cars once inside them, it is not known what makes martens associate with cars in the first place. We radio-tracked 13 urban stone martens over a 2-year period in Luxembourg to determine the context in which martens visit parked cars at night. Martens associated with cars most frequently in spring and early summer, when their activity on roads was highest and when they systematically patrolled and scent-marked cars. Most visits to cars were of short duration. We suggest, therefore, that the main factor promoting marten–car contact is territorial behaviour, rather than either the thermal benefits to be gained from proximity to recently used engines or the need for a safe environment for resting, hiding or food consumption.