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排序方式: 共有256条查询结果,搜索用时 15 毫秒
81.
Tamara Leahy Jessica P. Rickard Taylor Pini Bart M. Gadella Simon P. de Graaf 《Proteomics》2020,20(12)
Quantitative proteomic studies are contributing greatly to the understanding of the spermatozoon through the provision of detailed information on the proteins spermatozoa acquire and shed in the acquisition of fertility. Extracellular vesicles (EVs) are thought to aid in the delivery of proteins to spermatozoa in the male reproductive tract. The aim of this study is to isolate, identify and quantify EV proteins isolated from ram seminal plasma. Ram sperm plasma membrane proteins are also isolated using nitrogen cavitation and identified to better understand the interplay of proteins between the sperm membrane and extracellular environment. The categorization of proteins enriched in the EV population according to their function revealed three main groupings: vesicle biogenesis, metabolism, and membrane adhesion and remodeling. The latter group contains many reproduction‐specific proteins that show demonstrable links to sperm fertility. Many of these membrane‐bound proteins show testicular expression and are shed from the sperm surface during epididymal maturation (e.g., testis expressed 101; TEX101 and lymphocyte Antigen 6 Family Member K; LY6K). Their association with seminal EVs suggests that EVs may not only deliver protein cargo to spermatozoa but also assist in the removal of proteins from the sperm membrane. 相似文献
82.
Jesse E. Hanson Hank La Emile Plise Yung-Hsiang Chen Xiao Ding Taleen Hanania Emily V. Sabath Vadim Alexandrov Dani Brunner Emer Leahy Pascal Steiner Lichuan Liu Kimberly Scearce-Levie Qiang Zhou 《PloS one》2013,8(7)
Suberoylanilide hydroxamic acid (SAHA) is an inhibitor of histone deacetylases (HDACs) used for the treatment of cutaneous T cell lymphoma (CTCL) and under consideration for other indications. In vivo studies suggest reducing HDAC function can enhance synaptic function and memory, raising the possibility that SAHA treatment could have neurological benefits. We first examined the impacts of SAHA on synaptic function in vitro using rat organotypic hippocampal brain slices. Following several days of SAHA treatment, basal excitatory but not inhibitory synaptic function was enhanced. Presynaptic release probability and intrinsic neuronal excitability were unaffected suggesting SAHA treatment selectively enhanced postsynaptic excitatory function. In addition, long-term potentiation (LTP) of excitatory synapses was augmented, while long-term depression (LTD) was impaired in SAHA treated slices. Despite the in vitro synaptic enhancements, in vivo SAHA treatment did not rescue memory deficits in the Tg2576 mouse model of Alzheimer’s disease (AD). Along with the lack of behavioral impact, pharmacokinetic analysis indicated poor brain availability of SAHA. Broader assessment of in vivo SAHA treatment using high-content phenotypic characterization of C57Bl6 mice failed to demonstrate significant behavioral effects of up to 150 mg/kg SAHA following either acute or chronic injections. Potentially explaining the low brain exposure and lack of behavioral impacts, SAHA was found to be a substrate of the blood brain barrier (BBB) efflux transporters Pgp and Bcrp1. Thus while our in vitro data show that HDAC inhibition can enhance excitatory synaptic strength and potentiation, our in vivo data suggests limited brain availability may contribute to the lack of behavioral impact of SAHA following peripheral delivery. These results do not predict CNS effects of SAHA during clinical use and also emphasize the importance of analyzing brain drug levels when interpreting preclinical behavioral pharmacology. 相似文献
83.
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85.
New light on the integrin switch 总被引:1,自引:0,他引:1
Leahy DJ 《Structure (London, England : 1993)》2002,10(9):1152-1154
86.
Y Deng J Zhao D Sakurai KM Kaufman JC Edberg RP Kimberly DL Kamen GS Gilkeson CO Jacob RH Scofield CD Langefeld JA Kelly ME Alarcón-Riquelme BIOLUPUS GENLES Networks JB Harley TJ Vyse BI Freedman PM Gaffney KM Sivils JA James TB Niewold RM Cantor W Chen BH Hahn EE Brown PROFILE BP Tsao 《Arthritis research & therapy》2012,14(Z3):A5
87.
Dunne JC Li D Kelly WJ Leahy SC Bond JJ Attwood GT Jordan TW 《Journal of proteome research》2012,11(1):131-142
Plant polysaccharide-degrading rumen microbes are fundamental to the health and productivity of ruminant animals. Butyrivibrio proteoclasticus B316(T) is a gram-positive, butyrate-producing anaerobic bacterium with a key role in hemicellulose degradation in the rumen. Gel-based proteomics was used to examine the growth-phase-dependent abundance patterns of secreted proteins recovered from cells grown in vitro with xylan or xylose provided as the sole supplementary carbon source. Five polysaccharidases and two carbohydrate-binding proteins (CBPs) were among 30 identified secreted proteins. The endo-1,4-β-xylanase Xyn10B was 17.5-fold more abundant in the culture medium of xylan-grown cells, which suggests it plays an important role in hemicellulose degradation. The secretion of three nonxylanolytic enzymes and two CBPs implies they augment hemicellulose degradation by hydrolysis or disruption of associated structural polysaccharides. Sixteen ATP-binding cassette (ABC) transporter substrate-binding proteins were identified, several of which had altered relative abundance levels between growth conditions, which suggests they are important for oligosaccharide uptake. This study demonstrates that B. proteoclasticus modulates the secretion of hemicellulose-degrading enzymes and ATP-dependent sugar uptake systems in response to growth substrate and supports the notion that this organism makes an important contribution to polysaccharide degradation in the rumen. 相似文献
88.
Bond JJ Dunne JC Kwan FY Li D Zhang K Leahy SC Kelly WJ Attwood GT Jordan TW 《Journal of Proteomics》2012,75(11):3138-3144
The research was aimed at finding which membrane proteins of the rumen bacterium Butyrivibrio proteoclasticus are involved in the uptake of carbohydrates resulting from extracellular enzymatic degradation of hemicellulose and fructan. The proteomic analysis of cells grown with fructose or xylan as the sole substrate identified 13 membrane proteins predicted to function as carbohydrate transporters. One protein detected was the membrane component of a fructose-specific phosphoenolpyruvate:sugar phosphotransferase system believed to be involved in the fructose uptake following extracellular fructan breakdown. The other 12 proteins were all ABC transport system substrate-binding proteins, nine of which belong to functional category COG1653 that includes proteins predicted to transport oligosaccharides. Four of the SBPs were significantly upregulated in xylan grown cells, and three of these were found in polysaccharide utilisation loci where they are clustered with other genes involved in hemicellulose breakdown and metabolism. It is possible that the carbon source available regulates a wider network of genes. The information on the mechanisms used by rumen bacteria to take up carbohydrates from their environment may improve our understanding of the ruminant digestion and facilitate strategies for improved pasture and stored feed utilisation. 相似文献
89.
James D. Myers Thomas C. Allison Sandra Bittner Brett Didier Michael Frenklach William H. Green Jr. Yen-Ling Ho John Hewson Wendy Koegler Carina Lansing David Leahy Michael Lee Renata McCoy Michael Minkoff Sandeep Nijsure Gregor von Laszewski David Montoya Luwi Oluwole Carmen Pancerella Reinhardt Pinzon William Pitz Larry A. Rahn Branko Ruscic Karen Schuchardt Eric Stephan A. Wagner Theresa Windus Christine Yang 《Cluster computing》2005,8(4):243-253
The Collaboratory for Multi-scale Chemical Science (CMCS) is developing a powerful informatics-based approach to synthesizing
multi-scale information in support of systems-based research and is applying it within combustion science. An open source
multi-scale informatics toolkit is being developed that addresses a number of issues core to the emerging concept of knowledge
grids including provenance tracking and lightweight federation of data and application resources into cross-scale information
flows. The CMCS portal is currently in use by a number of high-profile pilot groups and is playing a significant role in enabling
their efforts to improve and extend community maintained chemical reference information.
James D. Myers received his B.A. in Physics from Cornell University in 1985 and his Ph.D. in Chemistry from the University of California
at Berkeley in 1993. He is currently the Associate Director for Collaborative Technologies at the National Center for Supercomputing
Applications (NCSA) at the University of Illinois, Urbana Champaign. Dr. Myers is the lead investigator on the U.S. Department
of Energy (DOE) sponsored Scientific Annotation Middleware project (http://www.scidac.org/SAM/) (scientific content management,
semantic annotation, and records functionality) and is serving as the Chief Technical Officer for the DOE-sponsored Collaboratory
for Multiscale Chemical Science (CMCS) project. His is also the lead architect for the Mid-America Earthquake Center's MAEViz
hazard risk management collaboratory and co-lead of NCSA's Collaborative Large-scale Engineering Analysis Network for Environmental
Research (CLEANER) related cybercollaboratory effort. Open source software developed by Dr. Myers and his colleagues including
the electronic laboratory notebook (ELN) and the Collaborative Research Environment (CORE) real-time collaboration environment
have been downloaded from the Pacific Northwest National Laboratory (PNNL) Collaboratory website (http://collaboratory.pnl.gov)
by thousands of researchers and educators. Due to space limitations, individual bios for all 28 authors are not shown. The
CMCS project is led by Dr. Larry Rahn (rahn@sandia.gov) at Sandia National Laboratories. The team includes combustion researchers
and computer science researchers and developers at five DOE National Laboratories (Argonne, Lawrence Livermore, Los Alamos,
Pacific Northwest, and Sandia National Laboratories), the National Institute of Standards and Technology, Massachusetts Institute
of Technology, and the University of California, Berkeley. Current contact information and biographic information for team
members is available at http://cmcs.org/team.php. 相似文献